Device for detecting statin-associated myopathy through single nucleotide polymorphism rs976754 and application

A single nucleotide polymorphism and detection device technology, applied in the field of molecular biology, can solve the problems of insufficient evidence and limited effect, and achieve the effects of improving the quality of life, reducing adverse drug reactions, and improving the quality of life of patients

Active Publication Date: 2019-11-12
BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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Problems solved by technology

Therefore, although the relationship between the SLCO1B1c.521T>C site mutation and the occurrence of myopathy has been proven, due to its limited effect, the evidence for clinical benefit of routine detection of this gene in patients is not sufficient, and most of the previous studi

Method used

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  • Device for detecting statin-associated myopathy through single nucleotide polymorphism rs976754 and application
  • Device for detecting statin-associated myopathy through single nucleotide polymorphism rs976754 and application
  • Device for detecting statin-associated myopathy through single nucleotide polymorphism rs976754 and application

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Experimental program
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Embodiment 1

[0074] Patients with ischemic stroke who were admitted to Beijing Tiantan Hospital Affiliated to Capital Medical University were selected. The dosage regimen of atorvastatin (Lipitor) for all patients was 80mg / d; no patient was using other lipid-lowering drugs and drugs affecting the metabolism of atorvastatin; no active liver and kidney disease. There is no other disease that can cause CK elevation, and no drugs that can cause CK elevation have been taken. According to the clinical data and follow-up results of the patients, 61 cases of myopathy were enrolled in the case group, and 110 cases of age-sex-matched control group without myopathy were enrolled.

[0075] 1. Massarray SNP typing experiment process

[0076] Such as figure 1 As shown, the Massarray SNP typing experiment process includes the following steps:

[0077] (1) Organize SNP sequence information into a standard format; (2) Input it into the software to design SNP unmarked primers to determine the target site...

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Abstract

The invention provides a statin-associated myopathy detecting device. The device performs Massarray SNP genotyping detection in accordance with a single nucleotide polymorphism site rs976754 of a sample to be detected. The device comprises the following composition mechanisms of a primer synthesizing mechanism, a sample detection quantifying mechanism, a PCR reaction mechanism, a single-base extension reaction mechanism and a computer detection and analysis mechanism. The genotype at the site has important directive significance in recognizing myopathy high-risk crowds and adjusting medicationat the early stage, and has important significance in screening myopathy risk genes at the early stage, forecasting the myopathy generation risk, providing individualized medication guidance, reducing the generation of adverse drug reactions, performing prognosis and outcome of primary diseases, and improving the life quality of patients needing to take statin type blood lipid reducing medicines.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a single nucleotide polymorphism rs976754 detection device and application for statin-related myopathy. Background technique [0002] Intracranial atherosclerosis is an independent risk factor for ischemic cerebrovascular disease, and hyperlipidemia can promote the formation of atherosclerotic plaque. Atorvastatin, as the first-line drug for lipid-lowering therapy, can competitively inhibit the rate-limiting enzyme 3-hydroxy-methylglutaryl CoA (HMG-CoA) reductase in cholesterol synthesis. Cholesterol synthesis is reduced, and at the same time, it promotes the increase of concentration-dependent LDL receptor activity, accelerates the catabolism of LDL, thereby significantly reducing blood lipid levels and stabilizing plaques, which is very beneficial to the prevention of ischemic cerebrovascular diseases. [0003] Usually the use of atorvastatin is effective and safe,...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11C12M1/34
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2600/106C12Q2531/113C12Q2533/101C12Q2565/627
Inventor 张丽敏张国军朱斌吕虹
Owner BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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