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SNP molecular markers, primer pairs and kit of FABP4 gene related to beef quality in Yanbian yellow cattle and application of SNP molecular marker, primer pair and kit

A technology of FABP4 and molecular markers, applied in the direction of DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of efficient utilization of Yanbian cattle

Active Publication Date: 2019-11-12
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the cultivation of new strains and varieties of Yanbian yellow beef has accelerated the industrialization process of Yanbian yellow cattle. However, due to the lag in research on genes and genetic markers related to important economic traits of Yanbian yellow cattle, the rapid development of advanced molecular breeding technologies at home and abroad development, and has not been efficiently utilized in the breeding process of Yanbian cattle breeds
However, there is no report on the polymorphism of the FABP4 gene and its related biological traits in Yanbian yellow cattle

Method used

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  • SNP molecular markers, primer pairs and kit of FABP4 gene related to beef quality in Yanbian yellow cattle and application of SNP molecular marker, primer pair and kit
  • SNP molecular markers, primer pairs and kit of FABP4 gene related to beef quality in Yanbian yellow cattle and application of SNP molecular marker, primer pair and kit
  • SNP molecular markers, primer pairs and kit of FABP4 gene related to beef quality in Yanbian yellow cattle and application of SNP molecular marker, primer pair and kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Sample collection

[0058] The test cattle group selected 70 Yanbian yellow cattle steers from Jilin Province Yanbian Animal Husbandry Development Group Co., Ltd., all the test cattle were fattened and slaughtered at the age of 30 months according to the unified national standard, and 25ml of blood was collected from the jugular vein of each cattle before slaughter. , add anticoagulant, aliquot and store at -20°C for later use.

[0059] 2. Extract DNA samples from Yanbian Yellow Cattle according to the instructions of the TIANGEN-Blood Genomic DNA Extraction Kit. The purity of the extracted Yanbian Yellow Cattle DNA sample was detected by ultraviolet spectrophotometer to determine the OD 260 / OD 280 value. Agarose gel electrophoresis detection: use 1% agarose gel to detect the Yanbian Yellow Cattle DNA sample, and perform voltage electrophoresis for 30 minutes according to the fragment length; observe with a gel imaging system.

[0060] The results of the ultravi...

Embodiment 2

[0104] 1. Experimental animals and sample collection

[0105] In this test cattle group, 70 Yanbian yellow cattle steers from Jilin Province Yanbian Animal Husbandry Development Group Co., Ltd. were selected. All the test cattle were fattened and slaughtered at the age of 30 months under the unified standard feeding conditions. Muscle, used to measure meat quality traits.

[0106] 2. Determination of main traits of cattle

[0107] (1) Determination of fat content: Soxhlet extraction method

[0108] The mass fraction of fat is calculated according to formula (1):

[0109]

[0110] In the formula: M is the mass of the sample, g; M1 is the mass after extraction, g; M2 is the mass before extraction, g.

[0111] (2)Marbling Rating

[0112] Refer to the Marbling Grading Chart (see Figure 8 ), observe the cross-section of the longissimus dorsi muscle, and evaluate the marbling grade of Yanbian cattle according to 6 grades, grade 1 is the best marbling grade, grade 6 is the wo...

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Abstract

The invention provides SNP molecular markers, primer pairs and a kit of FABP4 gene related to beef quality in Yanbian yellow cattle, and application of the SNP molecular markers, the primer pairs andthe kit, and belongs to the technical field of beef quality screening in yellow cattle. The SNP molecular markers of the FABP4 gene contain a nucleotide sequence of a polymorphism at the 3496 bp as anA / C site, a polymorphism at the 3533 bp as an A / T site, a polymorphism at the 3711 bp as a G / C site, a polymorphism at the 3745 bp as a T / C site and a polymorphism at the 3767 bp as a T / C site of theFABP4 gene. The above five polymorphic sites are located in the third exon, and are in a strong linkage disequilibrium state with similar genetic effects. When the SNP molecular markers are applied to screen breeding fat cattle, fat content and marble pattern are selected as the detection indicators for screening breeding fat cattle while the genotype is heterozygous.

Description

technical field [0001] The invention belongs to the technical field of yellow beef quality screening, and in particular relates to a FABP4 gene SNP molecular marker related to Yanbian yellow beef quality, a primer pair, a kit and an application thereof. Background technique [0002] With the continuous improvement of living standards, people have put forward higher requirements for meat quality, not only requiring meat quality to be fresh and tender, but also requiring meat quality to meet the standards of green food. Therefore, countries around the world have conducted extensive research on meat quality. "High-grade beef" and "fattening cattle" have become the current consumption fashion. [0003] Beef quality involves many indicators, and the definition of high-grade beef is different in each country. Generally, beef with good color, good freshness, high fat content, obvious marble shape, good tenderness and high edible value is called "high-grade beef". Developed countr...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 夏广军徐红艳徐畅邵静尹宝珍
Owner YANBIAN UNIV
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