Rapid detection test strip for acrylamide in food based on nano-gold composite coating

Abstract

The invention discloses an acrylamide rapid test strip in food based on a nano gold composite coating, and belongs to the field of food detection. The acrylamide rapid test strip is provided with a sample pad, a nucleic acid aptamer binding pad, a nitrocellulose membrane and a water absorption pad on a bottom plate. The sample pad is attached to the innermost end of the bottom plate. The nucleic acid aptamer binding pad is attached to the outer side of the sample pad. The nitrocellulose membrane is attached to the outer side of the nucleic acid aptamer binding pad. The water absorption pad isattached to the outer side of the nitrocellulose membrane. The water absorption pad is located at the outermost end of the bottom plate. The acrylamide rapid test strip is based on quantum size effectof gold nanoparticles, which means when the distance of the particles is smaller than the diameter of gold nanometers, the dipole moments of the adjacent particles overlap, agglomeration occurs, thecolor of the gold nanoparticles changes from red to purple or even blue. According to the invention, the gold nanoparticles are fixed to the test strip, the to-be-tested acrylamide visual detection isrealized and quantitative detection is realized through colorimetry, and the acrylamide rapid test strip in food based on the nano gold composite coating has the advantages of fastness, high sensitivity, simple operation and the like.

Description

technical field [0001] The invention relates to rapid detection of acrylamide in food, in particular to a rapid colorimetric test strip for detection of acrylamide in heat-processed food, in particular to a rapid detection test paper for acrylamide in food based on nano-gold composite coating, and belongs to the field of food detection. Background technique [0002] Acrylamide (Acrylamide) is a colorless, odorless small molecular toxic organic compound, which can cause damage to the genetic material DNA of cells. High-dose exposure can affect the nervous system and reproductive system of humans and animals, and has certain effects on rodents. Therefore, it is classified as a Group 2 carcinogen (Group 2A) by the International Agency for Research on Cancer (IARC). In April 2002, researchers from the Swedish National Food Administration and Stockholm University detected acrylamide in baked starchy foods for the first time. In recent years, acrylamide has been found to widely e...

AI Technical Summary

Problems solved by technology

However, the above two test strips based on the principle of immunochromatography can only conduct qualitative or semi-quantitative analysis of the target object, and cannot achieve accurate quantification, and the antibody is expensive, which increases the detection cost

Images