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Nucleic acid separation apparatus

A technology for nucleic acid separation and pressurized gas, which is applied in material separation, measuring devices, biochemical cleaning devices, etc., and can solve problems such as automation difficulties

Pending Publication Date: 2019-11-12
KURASHIKI BOSEKI KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, in the pretreatment process, there are multiple processes for dissolution treatment including reagent addition process, stirring process, heating process, etc., and automation is difficult

Method used

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  • Nucleic acid separation apparatus
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  • Nucleic acid separation apparatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0076] Figure 1A This is a schematic perspective plan view showing the planar structure of the top plate of the nucleic acid separation device 10 according to the first embodiment. Figure 1B is said to be Figure 1A A perspective schematic front view of the structure of the case where the front panel of the automatic nucleic acid isolation device 10 is seen through. figure 2 It is a block diagram showing the configuration of the nucleic acid isolation device 10 according to the first embodiment.

[0077] This nucleic acid isolation device 10 includes a dispensing tip / reagent setting unit 11 , a sample setting unit 12 , a heating / stirring unit 13 , a nucleic acid extraction unit 14 , a dispensing pressurizing unit 15 , and a control unit 16 .

[0078] In addition, the nucleic acid separation device 10 such as Figure 1A , Figure 1B and figure 2 As shown, a heating / stirring unit 13 is equipped with a stirring mechanism 51 including a stirring mechanism 51 that stirs a pro...

Embodiment approach 2

[0173] The planar structure of the case where the top plate of the nucleic acid separation device 10 of the second embodiment is seen through and the structure of the case where the front panel of the nucleic acid separation device 10 is seen through are substantially the same as those of the first embodiment. For example, if Figure 1A and Figure 1B shown. Figure 32 It is a block diagram showing the configuration of the nucleic acid isolation device 10 according to the second embodiment.

[0174] The nucleic acid isolation device 10 includes a dispensing tip / reagent setting unit 11 , a sample setting unit 12 , a heating / stirring unit 13 , a nucleic acid extraction unit 14 , a dispensing pressurizing unit 15 , a control unit 16 , and an ID management unit 17 . The ID management unit 17 includes a sample ID reading unit 18 and a collection tube ID reading unit 19 . The sample ID of the sample container 42 is read by the sample ID reading unit 18 . The collection tube ID of...

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Abstract

A nucleic acid separation apparatus is provided with a stirring mechanism for stirring a sample and a reagent both injected into a treatment tube to perform a dissolution treatment of the sample and the reagent, a pressurized gas supply mechanism for supplying a pressurized gas into a filter cartridge into which the sample solution that contains a nucleic acid and has been subjected to the dissolution treatment is injected for the purpose of separating and collecting the nucleic acid from the sample solution, a dispensing mechanism for injecting the sample, the reagent or the sample solution that has been subjected to the dissolution treatment into the treatment tube and the filter cartridge, and a transporting mechanism for transporting the pressurized gas supply mechanism and the dispensing mechanism, wherein a dispensing chip is provided in the dispensing mechanism in a detachable manner, and the dispensing mechanism is provided with a sucking / ejecting mechanism by which a liquid can be sucked and ejected through the dispensing chip and a liquid supply mechanism for ejecting the liquid through a nozzle.

Description

technical field [0001] The present invention relates to a nucleic acid separation device that automatically performs a pretreatment process of a sample solution containing nucleic acid to a nucleic acid extraction process. Background technique [0002] In recent years, techniques for extracting and analyzing nucleic acid (mainly DNA) containing genetic information of life from human blood, animal and plant tissues, etc. have been widely used. In order to extract DNA from blood, tissue, etc., various dedicated reagents, devices, etc. are required. [0003] Recently, the automation of the nucleic acid extraction process is being developed (for example, refer to Patent Document 1). On the other hand, in a so-called pretreatment process such as adding a reagent to blood, tissue, etc. and stirring it, it is necessary to process a plurality of reagents. In addition, various operations including reagent addition, stirring, and pressurization in the pretreatment process are still ...

Claims

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Application Information

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IPC IPC(8): C12M1/00G01N35/00G01N35/10
CPCB01L3/0279B01L2200/023B01L2300/0681G01N2030/8827G01N35/1009C12N15/1017G01N2035/103B01L2200/0631C12M47/06B01F31/22B01F31/26B01F35/71745B01F35/75465B01L3/0275B01L3/0293B01L2200/025B01L2200/16B01L2400/0487C12M47/12G01N35/1011G01N2030/009G01N2035/00277B01F27/05
Inventor 渡边健吾中川泰
Owner KURASHIKI BOSEKI KK