Application of grape seed proanthocyanidins in the preparation of combination drugs for cancer chemotherapy

A chemotherapeutic drug, proanthocyanidin technology, applied in the medical field, can solve the problem of not being able to fundamentally reduce cell drug resistance, cell drug resistance, etc., and achieve the effect of avoiding the increase of drug dosage, reducing risks, and reducing the increase of toxic and side effects

Active Publication Date: 2021-12-07
SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, targeted drug delivery can only improve the position of action of chemotherapeutic drugs, and cancer cells can still develop drug resistance after long-term drug use; Or administered at the same time, it still cannot fundamentally reduce cell drug resistance
That is to say, there is still no solution in the prior art that can fundamentally solve the problem of cell drug resistance in the process of cancer chemotherapy

Method used

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  • Application of grape seed proanthocyanidins in the preparation of combination drugs for cancer chemotherapy
  • Application of grape seed proanthocyanidins in the preparation of combination drugs for cancer chemotherapy
  • Application of grape seed proanthocyanidins in the preparation of combination drugs for cancer chemotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Analysis of protein expression in HL-60 cells and HL-60 / ADR cells

[0029] This example is an experiment for analyzing protein expression in HL-60 cells and HL-60 / ADR cells, and the experimental method is as follows.

[0030] 1.1 Extraction of total cell protein

[0031] 1.1.1 Instrument: high-speed / refrigerated centrifuge (Germany eppendorf, 5415D)

[0032] 1.1.2 Reagents:

[0033] (1) Whole protein lysate (Shanghai Kangcheng Bioengineering Co., Ltd.)

[0034] (2) Protease inhibitor mixture (Shanghai Kangcheng Bioengineering Co., Ltd.)

[0035] (3) PMSF solution (Shanghai Kangcheng Biological Engineering Co., Ltd.)

[0036] (4) Phosphatase Mixture (Shanghai Kangcheng Biological Engineering Co., Ltd.)

[0037] 1.1.3 Solution preparation

[0038] Protein extraction reagents:

[0039]

[0040] 1.1.4 Cell collection (HL-60 and HL-60 / ADR cells are suspension cells)

[0041] (1) HL-60 and HL-60 / ADR cells in 2×10 6 Inoculate in a 6cm culture dish. After...

Embodiment 2

[0081] Example 2: mRNA level analysis in HL-60 cells and HL-60 / ADR cells

[0082] This example is an experiment for analyzing protein expression in HL-60 cells and HL-60 / ADR cells, and the experimental method is as follows.

[0083] 2.1 Trizol extraction of total cellular RNA (050403A)

[0084] 2.1.1 Instruments and materials: high-speed / refrigerated centrifuge (Germany eppendorf, 5415D)

[0085] 2.1.2 Reagents:

[0086] (1) Trizol (2) DEPC water (3) Chloroform (4) Isopropanol (5) Ethanol

[0087] 2.1.3 Experimental steps

[0088] (1) Cell collection: a. HL-60 and HL-60 / ADR cells in 2×10 6 Inoculate in a 6cm culture dish. After the cells have grown for 48 hours, carefully transfer the cells into a 15ml centrifuge tube, centrifuge at 1500 rpm for 10 minutes to sediment the suspended cells, and discard the supernatant;

[0089] (2) Add 1ml PBS to the centrifuge tube, transfer the resuspended cells to a 1.5ml tube, centrifuge at 1500 rpm for 5 minutes and discard the superna...

Embodiment 3

[0147] Example 3: Analysis of the IC50 of 8 different drugs in HL-60 cells and HL-60 / ADR cells and the effect of grape seed proanthocyanidins on drug resistance reversal

[0148] This example is an analysis experiment of the IC50 of 8 different drugs in HL-60 cells and HL-60 / ADR cells and the effect of grape seed proanthocyanidins (hereinafter referred to as GSPE) in reversing drug resistance.

[0149] The 8 middle medicines shown in the present embodiment are used clinically commonly used cancer chemotherapeutic drugs, are respectively: doxorubicin (hereinafter denoted as ADR), daunorubicin (hereinafter denoted as DNR), etoposide (hereinafter denoted as VP16), vincristine (hereinafter referred to as VCR), cytarabine (hereinafter referred to as Ara-C), homoharringtonine (hereinafter referred to as HHT), mitoxantrone (hereinafter referred to as MTZ), pyridine Rubicin (hereinafter referred to as THP). The experimental method is as follows.

[0150] 3.1 Cell Treatment

[0151]...

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PUM

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Abstract

The purpose of the present invention is to provide a solution that can fundamentally reverse drug resistance so as to improve the curative effect of chemotherapy drugs and reduce their toxic and side effects. Specifically, the present invention provides the application of grape seed proanthocyanidins in the preparation of combined drugs for cancer chemotherapy regimens. The combined drugs are used in combination with chemotherapeutic drugs for treating cancer, thereby reducing the efficacy of chemotherapeutic drugs due to drug resistance. Reduce, or reduce the risk of toxic side effects that occur when the dose of chemotherapy drugs is increased due to drug resistance.

Description

technical field [0001] The invention belongs to the medical field and relates to an application of grape seed proanthocyanidins, in particular to the application of grape seed proanthocyanidins in the preparation of combined drugs for cancer chemotherapy. Background technique [0002] Chemotherapy is currently one of the main treatment methods for cancer, and its main implementation method is to administer chemotherapy drugs to induce apoptosis of cancer cells, so as to achieve the purpose of treatment. [0003] In clinical practice, long-term administration of chemotherapeutic drugs tends to cause drug resistance in patients, that is, the effective dose of chemotherapeutic drugs is increased. In this case, if the dose of chemotherapy drugs is not increased, the curative effect will be reduced; if the dose of chemotherapy drugs is increased, it will easily produce toxic side effects on normal cells. [0004] In the prior art, in order to delay the occurrence of drug resista...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/06A61K31/352A61K31/704A61K31/7068A61P35/00A61P35/02
CPCA61K31/352A61K31/704A61K31/7068A61K45/06A61P35/00A61P35/02A61K2300/00
Inventor 李浩林卡娜黄诗颖李兆春施芳红张顺国
Owner SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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