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Two-color ratiometric quantum dot airgel microsensor for glucose and its application

A micro-sensor, glucose technology, applied in chemical instruments and methods, material excitation analysis, selenium/tellurium compounds, etc. High sensitivity, the effect of realizing sensitivity

Active Publication Date: 2021-09-28
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the fluorescence intensity of single-emission quantum dots is easily affected by human operation and the environment, especially for visual detection, its fluorescence is interfered by ambient light and human subjective factors. Fluorescent quantum dots at different wavelengths are used to assemble composite materials for the detection of detection objects

Method used

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  • Two-color ratiometric quantum dot airgel microsensor for glucose and its application
  • Two-color ratiometric quantum dot airgel microsensor for glucose and its application
  • Two-color ratiometric quantum dot airgel microsensor for glucose and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Such as figure 1 with figure 2 A two-color ratiometric C / CdTe-GOx airgel microsensor for glucose is shown, including a microfluidic chip body, and the microfluidic chip includes a chip upper layer 100, a chip matrix substrate 300, a detection chamber 201, and a fluid inlet 101 and the fluid outlet 102, the fluid inlet 101 and the fluid outlet 102 are arranged on the chip layer 100, the detection chamber 201 is arranged between the chip layer 100 and the chip matrix substrate layer 300, and the two ends of the detection chamber 201 are connected to the fluid inlet 101 and the fluid outlet respectively. 102 are connected; the detection cavity 201 is provided with quantum dot airgel for detecting glucose; the material of the chip layer 100 is polydimethylsiloxane, UV resin or polymethyl methacrylate; the matrix substrate material is glass, silicon sheet or quartz; the chip layer 100 and the host substrate layer 300 are bonded by irreversible plasma oxidation or polymer m...

Embodiment 2

[0041] Application of the microsensor prepared in Example 2 in the visual qualitative detection of glucose

[0042] Mix glucose with PBS buffer to make 0mM, 4.3mM, 6.3mM, 8.7mM, 10.8mM, 13mM, 26mM glucose solutions, and inject 1.4μL of glucose solutions of various concentrations into the detection chamber through a microsampler After 5 minutes, use a 365nm ultraviolet lamp to irradiate the chip to observe its fluorescent color. According to the concentration of the glucose solution, the fluorescent color emitted by the chip is different, such as Figure 4 shown.

Embodiment 3

[0043] Application of the microsensor prepared in Example 3 in the quantitative detection of glucose

[0044] Mix glucose with PBS buffer to make 0mM, 4.3mM, 6.3mM, 8.7mM, 10.8mM, 13mM, 26mM glucose solutions, and inject 1.4μL of glucose solutions of various concentrations into the detection chamber through a microsampler After 5 minutes, irradiate the chip with a 365nm ultraviolet lamp and take its fluorescence image, read the RGB value of the fluorescence image and calculate its average R / G value, take the R / G value as the ordinate, and the glucose concentration C as the abscissa, and use origin9 to simulate get along like Figure 5 The microsensor detects glucose working curve.

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Abstract

The invention discloses a two-color ratio quantum dot airgel microsensor for glucose. The sensor includes a microfluidic chip body with a fluid inlet and a fluid outlet. detection chamber, and synthesized in the detection chamber a two-color ratiometric quantum dot airgel structure that can be used to detect glucose; this airgel structure can reflect different fluorescent colors for different glucose solutions, allowing people to pass through The glucose content of the measured solution was obtained by visual and instrumental analysis. The invention also discloses the application of the microsensor in visual qualitative detection and quantitative detection of glucose. The detection device of the present invention is portable, and can not only realize qualitative fast visual analysis, but also realize high-sensitivity quantitative analysis.

Description

technical field [0001] The invention relates to airgel sensor technology, in particular to a two-color ratio quantum dot airgel microsensor for glucose and its application. Background technique [0002] Fluorescent quantum dots are used as an ideal fluorescent material because their excited fluorescence has excellent spectral characteristics and stable photochemical properties, and its fluorescent color can be determined by its size without changing the surface composition. In the glucose photochemical enzyme biosensor. [0003] Quantum dot-based fluorescence detection methods can be divided into single emission type and ratiometric fluorescence type according to their signal output mode. The single-emission fluorescence detection method is a technique in which the fluorescence intensity of quantum dots with a single emission wavelength will increase or decrease due to the presence of the detection substance, and the quantitative detection of the detection substance can be ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C01B19/00
CPCC01B19/007G01N21/64
Inventor 倪中华胡涛叶亦徐康恺
Owner SOUTHEAST UNIV