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Preparation method of liquid fermented feed without anti-nutritional factors

A liquid fermentation and feed technology, applied in the field of animal husbandry, can solve problems such as limited application and shortage of feed resources, and achieve the effects of increasing growth rate, improving feed utilization rate, and easy absorption

Inactive Publication Date: 2019-11-29
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of animal husbandry, feed industry and food processing industry, staple food feed resources such as soybean meal and corn are becoming increasingly scarce; limiting their application in feed

Method used

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  • Preparation method of liquid fermented feed without anti-nutritional factors
  • Preparation method of liquid fermented feed without anti-nutritional factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Liquid strain preparation

[0033] Transfer the test-tube slant strains of Lactobacillus plantarum stored on YPD medium at 4°C to room temperature for activation for 4 hours; use sterile water to make the activated test-tube slant strains into bacterial suspension on a sterile operating table solution, rinsed into the Erlenmeyer flask with sterilized liquid medium, inoculated one Erlenmeyer flask with 1 test tube strain, and prepared the seed liquid by shaking culture for 24 hours; the cultured liquid seed liquid was inserted into the fermentation culture according to the inoculum size of 2%. culture medium at a temperature of 30°C for 24 hours to obtain a fermentation liquid of Lactobacillus plantarum with a bacterial quantity of 2×10 9 cfu / mL;

[0034] (2) Material pretreatment

[0035] The formula of the feed solids is: 20 parts of sweet potatoes, 5 parts of alfalfa, 5 parts of sweet potato seedlings, 20 parts of corn flour, 10 parts of soybean meal, 10 parts o...

Embodiment 2

[0039] (1) Liquid strain preparation

[0040] Transfer the test tube slant strains of Lactobacillus plantarum stored on YPD medium at 4°C to room temperature for activation for 6 hours; use sterile water to make the activated test tube slant strains into bacterial suspension solution, rinsed into the Erlenmeyer flask with sterilized liquid medium, inoculated one Erlenmeyer flask with 1 test tube strain, and prepared the seed liquid by shaking culture for 24 hours; the cultured liquid seed liquid was inserted into the fermentation culture according to the inoculation amount of 5%. culture medium at a temperature of 25°C for 24 hours to obtain a Lactobacillus plantarum fermentation broth with a bacterial count of 1.5×10 9 cfu / mL;

[0041] (2) Material pretreatment

[0042] The formula of feed solids is: 10 parts of sweet potato, 10 parts of pumpkin, 5 parts of alfalfa, 5 parts of peanut seedling, 15 parts of corn flour, 15 parts of soybean meal, 15 parts of rice bran, 25 parts...

Embodiment 3

[0046] (1) Liquid strain preparation

[0047] Transfer the test-tube slant strains of Lactobacillus plantarum stored on YPD medium at 4°C to room temperature for activation for 4 hours; use sterile water to make the activated test-tube slant strains into bacterial suspension on a sterile operating table solution, rinsed into the Erlenmeyer flask with sterilized liquid medium, inoculated one Erlenmeyer flask with 1 test tube strain, and prepared the seed liquid by shaking culture for 24 hours; the cultured liquid seed liquid was inserted into the fermentation culture according to the inoculation amount of 5%. culture medium at a temperature of 32°C for 24 hours to obtain a Lactobacillus plantarum fermentation broth with a bacterial count of 8×10 9 cfu / mL;

[0048] (2) Material pretreatment

[0049] The formula of feed solids is: 10 parts of sweet potato, 1 part of eucommia leaf, 4 parts of cabbage, 5 parts of alfalfa, 5 parts of sweet potato seedling, 15 parts of corn flour, ...

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Abstract

The invention belongs to the field of livestock breeding, and particularly relates to a preparation method of liquid fermented feed without anti-nutritional factors. The method mainly includes the following steps: (1) preparation of liquid bacteria; (2) pretreatment of materials; and (3) control in a fermentation process. According to the method provided by the invention, intestinal microbial community fermentation is selected aiming at nutritional requirements and intestinal health of fattening pigs, so that the content of probiotics and probiotic metabolites in the finished product is improved, the intestinal health level of the fattening pigs is improved, the disease prevention and disease resistance of the fattening pigs are enhanced, and the productivity of the fattening pigs is improved.

Description

technical field [0001] The invention belongs to the field of animal husbandry, and in particular relates to a method for preparing antibiotic-free liquid fermented feed. Background technique [0002] There are various ways of traditional liquid feed feeding. For example, in the past, individual households raised pigs in my country, leftovers and agricultural and sideline products were used as pig food and mixed with water to feed pigs in the form of liquid feed. This primitive feeding method is not prepared according to the scientific formula, and its nutrition is not balanced enough; and the hygienic conditions cannot be guaranteed, and it is difficult to control the growth and reproduction of harmful microorganisms in the liquid feed; this traditional feeding method is labor-intensive. Can not adapt to large-scale farming. [0003] Compared with traditional liquid feed, liquid feed in the modern sense refers to the metabolic activities of microorganisms themselves. By art...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K50/30A23K10/30A23K10/37
CPCA23K10/12A23K50/30A23K10/30A23K10/37Y02P60/87
Inventor 杨传伦马韵升吴文雷徐泽平刘结磊张心青马娜娜曹清杰
Owner CHAMBROAD CHEM IND RES INST CO LTD