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Method for removal of lead ions in sewage by mycobacterium phlei

A technology of Mycobacterium phlei and lead ions is applied in the field of heavy metal ion treatment in sewage, which can solve the problems such as the inability to achieve the recycling of adsorbents and heavy metals, and the limited number of heavy metal ions, and achieve good adsorption and removal effect, good social benefits and economic benefits. Efficient and easy to use in industrial applications

Inactive Publication Date: 2019-11-29
中交铁道设计研究总院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods can realize the adsorption and purification of some heavy metal ions, they generally have the disadvantages of being able to adsorb heavy metal ions, the types and quantities of which are limited, and that they cannot achieve the recovery and utilization of both adsorbents and heavy metals.

Method used

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  • Method for removal of lead ions in sewage by mycobacterium phlei
  • Method for removal of lead ions in sewage by mycobacterium phlei

Examples

Experimental program
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Effect test

Embodiment 1

[0050] The adsorption process of microbial adsorbent Mycobacterium phlei to copper ions in wastewater: add the obtained microbial adsorbent wet cells at a dosage of 9.0g / L to the flotation tank containing 60-200mg / L lead ion aqueous solution, The pH of the sewage is 4.0~7.0. After adding the wet cells of the microbial adsorbent, the flotation tank is stirred and adsorbed for 10 minutes, and then the saturated adsorbed microbial adsorbent is recovered and separated by flotation in the flotation tank. The stirring speed is 100-200r / min , pH=4.0~7.0, add 0.06mg / L dodecylamine to the Mycobacterium phlei suspension for 8min flotation, put the flotation recovered bacteria residue into 200mg / L sodium carbonate solution for desorption Regeneration, after the microbial adsorbent is regenerated and used for 4 times, the lead ion content in the water is lower than 40.0mg / L, and the concentration of the bacterial suspension in the flotation tank is reduced to below 0.01mg / L. Take the tail...

Embodiment 2

[0055] The effect of adding alkali metal cation solution in the flotation tank on the adsorption effect of the microbial adsorbent Mycobacterium phlei on the adsorption of lead ions in wastewater: the obtained microbial adsorbent wet cells were added to 60 In the flotation cell of -200mg / L lead ion aqueous solution, the pH of the sewage is 4.0~7.0, after adding the wet cells of the microbial adsorbent, add 5.3mg / L NH 4 Cl solution, the flotation tank was stirred and adsorbed for 10min. After the adsorption, the lead ion content in the water was lower than 20.0mg / L, and then the saturated adsorption microbial adsorbent was recovered and separated by flotation in the flotation tank. The stirring speed was 100-200r / min , pH=4.0~7.0, add 0.06mg / L collector dodecylamine to the suspension of Mycobacterium phlei, and after 8 minutes of flotation to recover the saturated microbial absorbent, the bacterial suspension in the flotation tank The concentration dropped below 0.01mg / L. Take...

Embodiment 3

[0060] Add the obtained microbial adsorbent wet cells into the flotation tank containing 60-200mg / L lead ion aqueous solution at a dosage of 9.0g / L. The pH of the sewage is 4.0~7.0. After putting in the microbial adsorbent wet cells, add NaCl solution 60.0mg / L and MgCl 2 Solution 95.0mg / L, the flotation tank was stirred and adsorbed for 10min, the lead ion content in the water after adsorption was lower than 20.0mg / L, and then the saturated adsorption microbial adsorbent was recovered and separated by flotation in the flotation tank, at a stirring speed of 100- Under the condition of 200r / min and pH=4.0~7.0, add 0.06mg / L collector dodecylamine to the bacterial suspension of Mycobacterium phlei, and recover the saturated microbial absorbent by flotation for 8min. The concentration of the bacterial suspension dropped below 0.01mg / L. Take the tail liquid in the flotation tank, and drain it after passing the test.

[0061] In the present invention, the collector dodecylamine cat...

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Abstract

The invention discloses a method for removal of lead ions in sewage by mycobacterium phlei. The method includes: subjecting a strain to continuous four-generation liquid culture activation in a nitrogen-containing liquid medium, then inoculating the product into an agar solid slant medium or agar solid plate medium for storage, inoculating the strain into a nitrogen-containing medium from the agarsolid slant medium or agar solid plate medium, conducting shaking culture at 28-30DEG C under 120r / min for 5d to obtain a microbial adsorbent, subjecting the microbial adsorbent to centrifugal precipitation or filtration to obtain microbial adsorbent wet cells, adding the microbial adsorbent wet cells into sewage containing lead ions in a flotation tank for adsorption flotation and recovery separation, and finally recovering the microbial adsorbent and the lead ions. The microbial adsorbent prepared by the method provided by the invention has good adsorption and removal effect on lead ions insewage, is low in cost, and is easy for industrial application.

Description

technical field [0001] The invention relates to the field of heavy metal ion treatment in sewage, in particular to a method for treating lead ions in sewage by using mycobacterium phlei. Background technique [0002] In the prior art, the heavy metal ions contained in the sewage are difficult to be biodegraded after entering the environment. Because of their high toxicity, they can migrate with the water after entering the water body, and finally accumulate in the organism through the food chain, destroying the normal physiology of the organism. Metabolic activities, and seriously threaten the surrounding ecological environment and human quality of life, so the treatment and resource utilization of heavy metal ion wastewater is one of the important research topics at present. As an environmentally friendly, efficient and economical new technology for sewage treatment and resource utilization, biotechnology has attracted increasing attention. [0003] Sewage has complex comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/20
CPCC02F3/34C02F2101/20
Inventor 周东琴周雨亭廖国才颜廷祥乔春江蒋富强杨慧林晏成
Owner 中交铁道设计研究总院有限公司
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