Ribose measurement method for spleen aminopeptide solution

A measurement method and solution technology, applied in the detection field, can solve problems such as inability to quantify, large error in experimental methods, and troublesome result judgment, and achieve the effect of intuitive and accurate results and guaranteed quality

Inactive Publication Date: 2019-11-29
DALIAN BAILITIANHUA PHARMA
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the principle of this method is the same as the orcinol method, it does not use an instrument to detect the value but uses a single-concentration visual colorimetric method. The experimental method has large errors, strong subj

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention will be further explained below in conjunction with specific examples.

[0014] 1. Preparation of reference substance solution: Accurately weigh an appropriate amount of D-ribose reference substance, dissolve it with 5% trichloroacetic acid solution and make a solution containing 20μg per 1ml, and shake well.

[0015] 2. Preparation of the test solution: Take 1ml of this product, add 5% trichloroacetic acid to 25ml, and make a solution containing 5μg of ribose per 1ml, as the test solution.

[0016] 3. Preparation of the standard curve Precisely measure 0.0ml, 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml of the reference substance solution into a stoppered test tube, add 5% trichloroacetic acid solution in an appropriate amount to 2.0ml, each Add 1% 3,5-dihydroxytoluene solution [take 1g of 3,5-dihydroxytoluene, add 0.1% ferric trichloride-hydrochloric acid solution (take 0.5g ferric chloride, add concentrated hydrochloric acid to dissolve into 500ml, long-te...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a ribose measurement method for a spleen aminopeptide solution. According to the method, when the spleen aminopeptide solution is prepared into a solution of a proper concentration by trichloroacetic acid, ribose contained by the spleen aminopeptide solution and concentrated hydrochloric acid are co-heated, the ribose is degraded and converted into furfural; the furfural reacts with 3, 5-dihydroxytoluene (orcinol), so that a bright green compound is generated under the catalysis of Fe <3+>; the reaction product has maximum absorbance at 650nm; when the concentration of the ribose is in a range of 20-250 microgramme.ml<-1>, light absorbance is in direct proportion to the concentration of the ribose; on the basis of the above principle, absorbance is measured at the wavelength of 650nm by adopting ultraviolet-visible spectrophotometry, a standard curve is drawn with the concentration adopted as an abscissa and the absorbance adopted as an ordinate; and a test sample is measured, and the content of the ribose is solved from a curve regression equation. The quantitative measurement method for the ribose in the spleen aminopeptide solution has the advantages of more accurate detection result and ensured product quality.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for measuring ribose in spleen aminopeptide solution. Background technique [0002] The ribose determination method of spleen aminopeptide solution is to dilute this product with trichloroacetic acid to a certain concentration, and when it is heated together with concentrated hydrochloric acid, it will be degraded and transformed into furfural, and then reacted with 3,5-dihydroxytoluene under the catalysis of Fe 3+ After reacting for a certain period of time, the color of the test product should not be lower than that of the reference product to be qualified. Although the principle of this method is the same as the orcinol method, it does not use an instrument to detect the value but uses a single-concentration visual colorimetric method. The experimental method has large errors, strong subjectivity, and cannot be quantified. During the experiment, sometimes the color of the sample ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/31G01N21/33
CPCG01N21/31G01N21/33
Inventor 刘琦秦伟韩树璘衣学龙
Owner DALIAN BAILITIANHUA PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap