Ribose measurement method for spleen aminopeptide solution

Abstract

The invention provides a ribose measurement method for a spleen aminopeptide solution. According to the method, when the spleen aminopeptide solution is prepared into a solution of a proper concentration by trichloroacetic acid, ribose contained by the spleen aminopeptide solution and concentrated hydrochloric acid are co-heated, the ribose is degraded and converted into furfural; the furfural reacts with 3, 5-dihydroxytoluene (orcinol), so that a bright green compound is generated under the catalysis of Fe <3+>; the reaction product has maximum absorbance at 650nm; when the concentration of the ribose is in a range of 20-250 microgramme.ml<-1>, light absorbance is in direct proportion to the concentration of the ribose; on the basis of the above principle, absorbance is measured at the wavelength of 650nm by adopting ultraviolet-visible spectrophotometry, a standard curve is drawn with the concentration adopted as an abscissa and the absorbance adopted as an ordinate; and a test sample is measured, and the content of the ribose is solved from a curve regression equation. The quantitative measurement method for the ribose in the spleen aminopeptide solution has the advantages of more accurate detection result and ensured product quality.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for measuring ribose in spleen aminopeptide solution. Background technique [0002] The ribose determination method of spleen aminopeptide solution is to dilute this product with trichloroacetic acid to a certain concentration, and when it is heated together with concentrated hydrochloric acid, it will be degraded and transformed into furfural, and then reacted with 3,5-dihydroxytoluene under the catalysis of Fe 3+ After reacting for a certain period of time, the color of the test product should not be lower than that of the reference product to be qualified. Although the principle of this method is the same as the orcinol method, it does not use an instrument to detect the value but uses a single-concentration visual colorimetric method. The experimental method has large errors, strong subjectivity, and cannot be quantified. During the experiment, sometimes the color of the sample ...

AI Technical Summary

Problems solved by technology

Although the principle of this method is the same as the orcinol method, it does not use an instrument to detect the value but uses a single-concentration visual colorimetric method. The experimental method has large errors, strong subjectivity, and cannot be quantified. During the experiment, sometimes the color of the sample will occur. It is darker than the reference product, but it is not a phenomenon of a color system, which makes it difficult to judge the result