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Method for extracting anthocyanin from lonicera caerulea

A kind of technology of anthocyanin and indigo fruit, applied in the direction of organic chemistry, can solve the problems of difficult large-scale production, expensive column packing, high production cost, etc., and achieve the effect of mild extraction process conditions, low production cost and environmental friendliness

Active Publication Date: 2019-12-03
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses methanol to extract and elute, which will lead to methanol residue in the product, and this method uses C18 column adsorption, the yield is small and the column packing is expensive, the production cost is high, and it is difficult to produce on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] This embodiment includes the following steps:

[0063] (1) Extraction: leaching 2000g of fresh blue indigo fruit (sampling in advance for inspection, the mass percentage of anthocyanin is 0.170%) is extracted in cold water at 20°C. Extraction, leaching for 8 hours each time, and stirring intermittently, after each leaching, pass through a 100-mesh filter screen to obtain an extract, combine the extracts and centrifuge to obtain a crude extract;

[0064] (2) Ultrafiltration: pass the crude extract obtained in step (1) through an ultrafiltration membrane with a molecular weight cut-off of 3KDa, and collect the permeate;

[0065] (3) Resin adsorption: The permeate collected in step (2) is filled with Dowex-50 cation exchange resin with a height-to-diameter ratio of 8:1 at a flow rate of 2BV / h until it is nearly saturated; The sodium chloride aqueous solution starts to linearly elute the resin from 0M, the elution volume is 10BV, and the elution flow rate is 3BV / h; after t...

Embodiment 2

[0069] (1) Extraction: leaching 2000g of frozen blue indigo fruit (sampling in advance for inspection, the mass percentage of anthocyanin is 0.165%) is extracted in cold water at 10°C, the amount of cold water is 40kg, and extracted in 4 times , leaching for 4 hours each time, and stirring intermittently, passing through a 100-mesh filter screen after each leaching to obtain an extract, combining the extracts and centrifuging to obtain a crude extract;

[0070] (2) Ultrafiltration: pass the crude extract obtained in step (1) through an ultrafiltration membrane with a molecular weight cut-off of 6KDa, and collect the permeate;

[0071] (3) Resin adsorption: The permeate collected in step (2) is saturated with D113 cation exchange resin with a height-to-diameter ratio of 6:1 at a flow rate of 1.5BV / h; then, use a concentration of 0-1.5M The potassium chloride aqueous solution starts to linearly elute the resin from 0M, the elution volume is 8BV, and the elution flow rate is 2BV / ...

Embodiment 3

[0075] (1) Extraction: Extract 1000g of dehydrated indigo fruit (sampled and sent for inspection in advance, the mass percentage of anthocyanin is 0.270%) in cold water at 4°C. Extraction, leaching 8h each time, and stirring intermittently. Pass through a 100-mesh filter each time to obtain an extract, combine the extracts and centrifuge to obtain a crude extract;

[0076] (2) Ultrafiltration: pass the crude extract obtained in step (1) through an ultrafiltration membrane with a molecular weight cut-off of 9KDa, and collect the permeate;

[0077] (3) Resin adsorption: use the permeate collected in step (2) at a flow rate of 0.3BV / h on the D131 cation exchange resin with a height-to-diameter ratio of 4:1 until it is nearly saturated; then, use chlorine with a concentration of 0-2M The sodium chloride aqueous solution starts to linearly elute the resin from 0M, the elution volume is 6BV, and the elution flow rate is 1BV / h. After the elution is completed, the eluent containing a...

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Abstract

The invention discloses a method for extracting anthocyanin from lonicera caerulea. The method comprises the following steps that (1) extraction is conducted, specifically, water is taken as a solventto extract the crushed lonicera caerulea, solid-liquid separation is conducted, and liquid is a crude extract; (2) ultrafiltration is conducted, specifically, the crude extract is ultrafiltered, andpermeating liquid is collected as upper column supernatant; (3) resin adsorption elution is conducted, specifically, cation exchange resin columns on the upper column supernatant are linearly eluted through a saline solution, eluent containing the anthocyanin is collected, and concentration is conducted to obtain a concentrated solution; and (4) crystallization is conducted, specifically, low alcohol is added into the concentrated solution, and after crystallization is completed, centrifuging, washing and drying are conducted to obtain a product. The anthocyanin product extracted from the lonicera caerulea through the method is high in anthocyanin content; and the whole extracting process is mild in condition, and the method has the characteristics of being high in yield, environmentally friendly, suitable for industrial production and the like.

Description

technical field [0001] The invention relates to a method for extracting anthocyanins, in particular to a method for extracting anthocyanins from Cystaceae. Background technique [0002] Indigo fruit, also known as Heixiazi fruit, is mainly distributed in Heilongjiang, Jilin, Liaoning and other places in China. The fruit tastes sour and sweet. It can be used for food, wine and medicine, and is an important wild resource in Northeast China. Indigo fruit is rich in nutrients, but also contains a large amount of active anthocyanins. At present, there are relatively few reports and researches on the extraction of anthocyanins from blue indigo fruit, mainly in the processing of fresh fruit into wine, Juice, jam, preserved fruit and jelly, so the extraction of anthocyanins from them is very promising. [0003] CN105693680 discloses a method for extracting the anthocyanins of the indigo fruit. In the method, the raw material of the indigo fruit is washed and removed, squeezed and ...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 何安乐李伟黄华学
Owner HUNAN HUACHENG BIOTECH
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