Preparation method, product and application of carboxymethylated xylan
A technology for carboxymethylation of wood and xylan, applied in the field of polysaccharide modification and modification, can solve the problem of not having the effect of promoting the proliferation of probiotics in vitro and the like
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Embodiment 1
[0033] The carboxymethylated xylan was prepared by the chemical method of sodium hydroxide-chloroacetic acid. 6 g of xylan was weighed, dissolved in 100 mL of isopropanol and 50 mL of 20% NaOH solution, and stirred under an ice bath for 3 h to obtain solution A, 30 g of chloroacetic acid was dissolved in 100 mL of isopropanol, and mixed with 50 mL of 20% NaOH solution to obtain mixed solution B. The mixed solution B was slowly dropped into the solution A, stirred at 60 °C for 4 h, the reaction was stopped, cooled to room temperature, neutralized to neutrality with 1 mol·L-1 hydrochloric acid, dialyzed under running water for 24 h, the sample solution was evaporated and concentrated, and then freeze-dried. , to obtain carboxymethylated xylan.
[0034] Infrared spectroscopy
[0035] Take 1 mg of dried carboxymethylated xylan and 1 mg of xylan polysaccharide, respectively, mix with 100 mg of KBr reference substance, grind, and press into tablets at 4000-400 cm -1 The infrared s...
Embodiment 2
[0041]The carboxymethylated xylan was prepared by the chemical method of sodium hydroxide-chloroacetic acid. 3 g of xylan was weighed, dissolved in 100 mL of isopropanol and 50 mL of 20% NaOH solution, and stirred under ice bath for 3 h to obtain solution A, 15 g of chloroacetic acid was dissolved in 100 mL of isopropanol, and mixed with 50 mL of 20% NaOH solution to obtain mixed solution B. The mixed solution B was slowly dropped into solution A, stirred at 30 °C for 2 h, the reaction was stopped, cooled to room temperature, neutralized to neutrality with 1 mol·L-1 hydrochloric acid, dialyzed under running water for 24 h, the sample solution was evaporated and concentrated, and then freeze-dried. , to obtain a carboxymethylated xylan with a degree of substitution of 0.43.
Embodiment 3
[0043] The carboxymethylated xylan was prepared by the chemical method of sodium hydroxide-chloroacetic acid. 6 g of xylan was weighed, dissolved in 100 mL of isopropanol and 50 mL of 20% NaOH solution, and stirred under an ice bath for 3 h to obtain solution A, 20 g of chloroacetic acid was dissolved in 100 mL of isopropanol, and mixed with 50 mL of 20% NaOH solution to obtain mixed solution B. The mixed solution B was slowly dropped into solution A, stirred at 60 °C for 5 h, the reaction was stopped, cooled to room temperature, neutralized to neutrality with 1 mol·L-1 hydrochloric acid, dialyzed under running water for 24 h, the sample solution was evaporated and concentrated, and then freeze-dried. , to obtain a carboxymethylated xylan with a degree of substitution of 1.18.
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