Preparation method, product and application of carboxymethylation xylan
A carboxymethylated wood and xylan technology, applied in the field of polysaccharide modification, can solve the problem of not having the effect of promoting the proliferation of probiotics in vitro
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Embodiment 1
[0033] Carboxymethylated xylan was prepared by sodium hydroxide-chloroacetic acid chemical method, and 6 g of xylan was weighed, dissolved in 100 mL of isopropanol and 50 mL of 20% NaOH solution, and stirred for 3 hours in an ice bath to obtain solution A. Dissolve 30 g of chloroacetic acid in 100 mL of isopropanol and mix with 50 mL of 20% NaOH solution to obtain mixed solution B. Slowly drop the mixed solution B into solution A, stir at 60°C for 4 hours, stop the reaction, cool to room temperature, neutralize to neutral with 1mol L-1 hydrochloric acid, dialyze with running water for 24 hours, evaporate and concentrate the sample solution and then freeze-dry , to obtain carboxymethylated xylan.
[0034] Infrared Spectroscopy Analysis
[0035] Take 1 mg of dried carboxymethylated xylan and 1 mg of xylan polysaccharide, mix them with 100 mg of KBr reference substance respectively, grind them, and press them into tablets. -1 Scope is carried out infrared spectrum scanning with...
Embodiment 2
[0041]Carboxymethylated xylan was prepared by sodium hydroxide-chloroacetic acid chemical method, 3g of xylan was weighed, dissolved in 100mL of isopropanol and 50mL of 20% NaOH solution, and stirred for 3 hours under ice bath to obtain solution A. Dissolve 15 g of chloroacetic acid in 100 mL of isopropanol and mix with 50 mL of 20% NaOH solution to obtain a mixed solution B. Slowly drop the mixed solution B into the solution A, stir at 30°C for 2 hours, stop the reaction, cool to room temperature, neutralize with 1mol L-1 hydrochloric acid, dialyze with running water for 24 hours, freeze-dry the sample solution after evaporation and concentration , to obtain carboxymethylated xylan with a degree of substitution of 0.43.
Embodiment 3
[0043] Carboxymethylated xylan was prepared by sodium hydroxide-chloroacetic acid chemical method, and 6 g of xylan was weighed, dissolved in 100 mL of isopropanol and 50 mL of 20% NaOH solution, and stirred for 3 hours in an ice bath to obtain solution A. Dissolve 20 g of chloroacetic acid in 100 mL of isopropanol and mix with 50 mL of 20% NaOH solution to obtain a mixed solution B. Slowly drop the mixed solution B into the solution A, stir at 60°C for 5h, stop the reaction, cool to room temperature, neutralize to neutral with 1mol L-1 hydrochloric acid, dialyze with running water for 24h, evaporate and concentrate the sample solution and then freeze-dry , to obtain carboxymethylated xylan with a degree of substitution of 1.18.
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