Bacillus amyloliquefaciens YB-130, and microbial preparation and application thereof

A technology of YB-130 and amylolytic spores, applied in the direction of microorganisms, microorganisms, biocides, etc., can solve the problems of inability to utilize or tap the potential of the main variety resources, long breeding cycle, high cost, etc., and achieve low production costs and long-term functional Prolong and improve the effect of yield and quality

Active Publication Date: 2019-12-10
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Wheat grain and its flour quality are directly related to variety breeding and improvement; therefore, breeding new high-quality wheat varieties is one of the important ways to improve wheat quality; however, the long breeding cycle and high cost make it difficult to solve the immediate problems Practical problems, and it is impossible to utilize or tap the potential of the current popular main species resources

Method used

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  • Bacillus amyloliquefaciens YB-130, and microbial preparation and application thereof
  • Bacillus amyloliquefaciens YB-130, and microbial preparation and application thereof
  • Bacillus amyloliquefaciens YB-130, and microbial preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Isolation, screening and identification of Bacillus amyloliquefaciens YB-130

[0032] (1) Sample collection

[0033] On May 15, 2016, Yang Lirong collected diseased ears of wheat in the wheat field of Xihua Institute of Agricultural Sciences, Songpo Village, Chiying Township, Xihua County, and brought them back in fresh-keeping bags for later use.

[0034] (2) Separation and purification

[0035] The collected ear tissue of wheat plants was taken as the object of endophytic bacteria isolation.

[0036] Grains from different parts of ears were collected at the filling stage, and 1 g samples were washed with running water for several times, dried naturally, soaked in 75% ethanol for 1 min, 2% NaClO for 3 min, 75% ethanol for 30 s, and no Bacterial water was rinsed twice again, and the NA medium plate was coated with the water washed for the last time to check the sterilization effect. The surface sterilized and clean samples were placed on the plate, and ...

Embodiment 2

[0059] Embodiment 2: the cultivation of bacillus amyloliquefaciens YB-130

[0060] Specific steps are as follows:

[0061] (1) Petri dish culture: inoculate Bacillus amyloliquefaciens YB-130 on solid medium LB under sterile conditions, and culture at 28-32°C, preferably 30°C, for 32-48 hours;

[0062] (2) Primary seed culture: Inoculate the bacteria cultured in step (1) in the primary seed medium under sterile conditions, with an initial pH value of 7.0-7.2, and cultivate at 24-37°C for 20-24 hours , to produce first-class seeds;

[0063] The formula of the primary seed medium is: corn flour 10g, glucose 5g, bean cake powder 15g, fish meal 5g, CaCO 3 5g, (NH 4 ) 2 SO 4 1g, K 2 HPO 4 0.3g, MgSO 4 ·7H 2 O 0.2g, water 1000ml;

[0064] (3) Secondary seed cultivation: inoculate the first-stage seeds into the fermentation medium with an inoculation amount of 1-6% by volume, aerate, and cultivate for 28-40 hours to obtain second-stage seeds;

[0065] The formula of the s...

Embodiment 3

[0067] Embodiment 3: the preparation of bacillus amyloliquefaciens YB-130 microbial preparation

[0068] (1) Mix the concentrated bacterial solution prepared in Example 2 with talcum powder and attapulgite, and spray dry to make mother powder;

[0069] (2) Add maltodextrin to mother powder to prepare microbial preparations;

[0070] Among them, the mass percentages of bacterial liquid, maltodextrin and bentonite are 60%, 8% and 1%, and talcum powder and attapulgite make up 100%. The effective number of viable bacteria of Bacillus amyloliquefaciens YB-130 is 100 billion / g.

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Abstract

The invention discloses bacillus amyloliquefaciens YB-130 as well as a microbial preparation and application thereof. The purpose of the invention is to solve the technical problems of poor quality, low yield and disease susceptibility of wheat. The bacillus amyloliquefaciens YB-130 screened out by the invention is named in the classification Bacillus amyloliquefaciens, and is preserved with preservation number CGMCC NO. 16229; and moreover, the invention provides a microbial preparation which is prepared from the following raw materials in percentage by mass: 60-80% of bacterium fluid of thebacillus amyloliquefaciens CGMCC NO. 16229, 8-10% of an adhesive, 1-2% of a stabilizer, and the balance of a filler so as to make the total amount 100%. The bacillus amyloliquefaciens YB-130 and the microbial preparation thereof have good application effects in the aspects of wheat quality and yield improvement, fusarium graminearum DON toxin synthesis inhibition, biological control and the like.The microbial preparation disclosed by the invention is capable of effectively improving yield and quality of wheat cultivated in filed; and moreover, the microbial preparation is also capable of realizing green prevention and control of crop diseases.

Description

technical field [0001] The invention relates to the technical field of microbial preparations, in particular to a bacillus amyloliquefaciens YB-130, microbial preparations and applications thereof. Background technique [0002] Wheat is one of the most important food crops in the world. In northern China, wheat is the crop with the largest planting area and plays a decisive role in agricultural production. [0003] With the improvement of the quality of life, people have higher and higher requirements for the quality of wheat. At this stage, my country's total wheat production has basically met people's needs, and the supply has changed from a long-term shortage to a basic balance in the total amount, which is more than enough in good years. People have higher requirements for the quality of wheat grain, and the demand for it has shifted from solving food and clothing to a new stage of high quality, safety and health. The main contradiction of wheat has changed from insuffi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCC12N1/20A01N63/00C12R2001/07C12N1/205
Inventor 杨丽荣夏明聪张洁张立勇孙润红潘娅梅武超
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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