Accurate printing construction method for liver unit chip containing acinus three-vessel structure

Abstract

The invention discloses an accurate printing construction method for a liver unit chip containing an acinus three-vessel structure, and belongs to the field of microfluidic technologies, tissue engineering and biological three-dimensional (3D) printing. According to the method, hydrogel containing cells constructs a liver tissue model in a microfluidic chip through a biological 3D printer, and theliver cell hydrogel model is subjected to perfusion culture through structural design of the microfluidic chip to simulate the liver microenvironment of a human body; and the chip comprises multiplelayers and contains three types of different micro-channel structures so as to reproduce a three-vessel system of the hepatic arteries, portal veins and central veins in liver lobules. The accurate printing construction method provides a more accurate analytical testing platform for drug development and liver drug toxicity testing.

Description

technical field [0001] The invention relates to a precise printing and construction method of a liver unit chip containing acinar three-vessel structure, which is used to simulate the three-vessel system of the hepatic artery-portal vein-central vein in the hepatic lobule and is used for drug development and liver drug toxicity testing, belonging to The fields of microfluidics, tissue engineering, and three-dimensional (3D) printing of biology. Background technique [0002] As the most important organ performing metabolic functions in the body, the liver undertakes more than 500 important physiological functions such as metabolic detoxification, immune coagulation, and protein synthesis. Therefore, constructing a tissue model that can simulate liver function in vitro is of great significance for drug development, drug safety testing, and liver disease research. [0003] The hepatic acinus is considered to be the smallest structural unit of the liver, as well as the smallest...

AI Technical Summary

Problems solved by technology

However, the existing liver-on-a-chip technology still has many deficiencies, so new research methods are urgently needed to study the construction of the in vitro microenvironment of the liver unit

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