Accurate printing construction method for liver unit chip containing acinus three-vessel structure

A construction method and vascular technology, applied in artificial cell constructs, biochemical equipment and methods, hepatocytes, etc., to achieve the effect of maintaining stable mechanical properties of the structure

Active Publication Date: 2019-12-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the existing liver-on-a-chip technology still has many deficiencies, so new research methods are urgently needed to study the construction of the in vitro microenvironment of the liver unit

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  • Accurate printing construction method for liver unit chip containing acinus three-vessel structure

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Embodiment Construction

[0028] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

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Abstract

The invention discloses an accurate printing construction method for a liver unit chip containing an acinus three-vessel structure, and belongs to the field of microfluidic technologies, tissue engineering and biological three-dimensional (3D) printing. According to the method, hydrogel containing cells constructs a liver tissue model in a microfluidic chip through a biological 3D printer, and theliver cell hydrogel model is subjected to perfusion culture through structural design of the microfluidic chip to simulate the liver microenvironment of a human body; and the chip comprises multiplelayers and contains three types of different micro-channel structures so as to reproduce a three-vessel system of the hepatic arteries, portal veins and central veins in liver lobules. The accurate printing construction method provides a more accurate analytical testing platform for drug development and liver drug toxicity testing.

Description

technical field [0001] The invention relates to a precise printing and construction method of a liver unit chip containing acinar three-vessel structure, which is used to simulate the three-vessel system of the hepatic artery-portal vein-central vein in the hepatic lobule and is used for drug development and liver drug toxicity testing, belonging to The fields of microfluidics, tissue engineering, and three-dimensional (3D) printing of biology. Background technique [0002] As the most important organ performing metabolic functions in the body, the liver undertakes more than 500 important physiological functions such as metabolic detoxification, immune coagulation, and protein synthesis. Therefore, constructing a tissue model that can simulate liver function in vitro is of great significance for drug development, drug safety testing, and liver disease research. [0003] The hepatic acinus is considered to be the smallest structural unit of the liver, as well as the smallest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12M3/06C12Q1/02
CPCC12M21/08C12M23/16C12M29/04C12M29/10C12N5/0697C12N2502/086C12N2502/1157C12N2502/14C12N2503/04C12N2513/00C12N2533/30C12N2533/54G01N33/5067G01N2500/10
Inventor 马梁吴钰桐李雨亭张斌杨华勇
Owner ZHEJIANG UNIV
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