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Method of increasing yield of L-isoleucine

A technology of isoleucine and yield, which is applied in the field of increasing the yield of L-isoleucine, can solve the problems of long fermentation period, low acid production rate, low product quality, etc., and achieve increased yield, easy operation, and simple process Effect

Active Publication Date: 2019-12-10
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The domestic amino acid industry should attach great importance to the application of biochemical technology, solve the problems of long fermentation cycle, low acid production rate and low product quality of L-isoleucine as soon as possible, and carry out research on the application of isoleucine in new fields , to promote the development of my country's L-isoleucine industry

Method used

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  • Method of increasing yield of L-isoleucine
  • Method of increasing yield of L-isoleucine
  • Method of increasing yield of L-isoleucine

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A method for increasing the output of L-isoleucine, comprising the steps of:

[0030] Using Corynebacterium glutamicum ATCC14309 as the experimental strain, Corynebacterium glutamicum seed solution (OD 600 The value is 12) According to the inoculation amount of 6%, transfer to a 50L fermenter containing 30L fermentation medium for cultivation, the temperature is 30°C, the cultivation time is 60 hours, and the pH of the medium is controlled by automatically adding 20% ​​ammonia water to 6.8-7.0. Control the dissolved oxygen at 25% by stirring and ventilating, add glucose nutrient solution, and control the residual sugar content in the fermentation broth to not be less than 1g / L;

[0031] During the fermentation process, 0.2 L of H 2 o 2 ; In the time of 48h, add chitosan in fermenter, control the concentration of chitosan to be 40mg / L;

[0032] The fermentation medium component is: glucose 80g / L, (NH 4 ) 2 SO 4 20g / L, corn steep liquor 10g / L, KH 2 PO 4 5g / L, Mg...

Embodiment 2

[0035] A method for increasing the output of L-isoleucine, comprising the steps of:

[0036] Using Corynebacterium glutamicum ATCC14309 as the experimental strain, Corynebacterium glutamicum seed solution (OD 600 The value is 11) According to the inoculum amount of 7%, it is transferred to a 50L fermenter containing 30L fermentation medium for cultivation, the temperature is 30°C, the cultivation time is 60 hours, and the pH of the medium is controlled by automatically adding 20% ​​ammonia water to 6.8-7.0. Control the dissolved oxygen at 20% by stirring and ventilating, add glucose nutrient solution, and control the residual sugar content in the fermentation broth to not be less than 1g / L;

[0037] During the fermentation process, 0.15 L of H 2 o 2; In the time of 48h, add chitosan in fermenter, control the concentration of chitosan to be 40mg / L;

[0038] The fermentation medium component is: glucose 80g / L, (NH 4 ) 2 SO 4 20g / L, corn syrup 10g / L, KH 2 PO 4 5g / L, MgS...

Embodiment 3

[0055] 1.H 2 o 2 Effects of addition amount and timing on L-isoleucine production and cell biomass in fermentation broth.

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Abstract

The invention belongs to the technical field of L-isoleucine production and discloses a method of increasing the yield of L-isoleucine. The method comprises the following step of adding H2O2 and a glucose nutrient solution. In the method, by optimizing a fermentation method, the yield of L-isoleucine is increased.

Description

technical field [0001] The invention belongs to the technical field of L-isoleucine production, and in particular relates to a method for increasing the output of L-isoleucine. Background technique [0002] L-isoleucine, also known as isoleucine, chemical name L-2-amino-3 methylvaleric acid, in 1901, Fischer discovered optical activity in the L-leucine fraction separated from protein hydrolyzate This is the earliest report about L-isoleucine. L-isoleucine is an important part of living organisms and belongs to essential amino acids, because its special structure and function play an important role in human life metabolism. If the human body lacks L-isoleucine for a long time, it will affect the physiological function of the body, leading to metabolic disorders and decreased resistance. With its unique efficacy, L-isoleucine is widely used in food health care, biomedicine, medical beauty and other industries. [0003] The production scale of isoleucine in my country is sma...

Claims

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Application Information

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IPC IPC(8): C12P13/06C12N1/38C12N1/20C12R1/15
CPCC12P13/06C12N1/38C12N1/20
Inventor 包鑫李江涛张宗华田辉冯世红李江雷
Owner XINJIANG FUFENG BIOTECH
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