Biological/chemical detection reagents and detection methods based on hollow gold nanoparticles-DNA complexes

Abstract

The invention discloses a biological / chemical target detection reagent and a detection method based on a hollow gold nanoparticle-DNA complex, and belongs to the technical field of analytical chemistry and biological detection. The invention uses carcinoembryonic antigen as a template target molecule, and co-modifies the DNA sequence (rpDNA) complementary to carcinoembryonic antigen nucleic acid aptamer and horseradish peroxidase (HRP) on hollow gold nanoparticles to prepare gold nanocomposite Then, the gold nanocomplexes are modified on the micro-nano magnetic beads by complementary hybridization of rpDNA and nucleic acid aptamers to prepare carcinoembryonic antigen detection reagents / sensors. The detection reagent is incubated with the carcinoembryonic antigen solution to be tested for 1 hour to rapidly release the hollow gold nanocomplexes, and finally the released gold nanocomplexes are subjected to naked-eye visualization, ultraviolet-visible-near-infrared spectroscopy and electrochemical techniques as required. Different modes of detection to determine the level of carcinoembryonic antigen. The invention realizes the simple, rapid, sensitive, specific, precise and accurate detection of standard samples and human serum samples, does not require complicated operation steps and expensive instruments and equipment, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry and biological detection, and more specifically relates to a biological / chemical target detection reagent and detection method based on a hollow gold nanoparticle-DNA complex. Background technique [0002] The detection of biological or chemical targets has very important applications in the fields of environmental monitoring, clinical diagnosis, food inspection and scientific research. Establishing sensitive, simple, and low-cost detection methods for detecting biological or chemical targets has always been a research hotspot in the field of analytical chemistry and biological detection technology, and is also a requirement for on-site instant detection. Nanoparticles have the advantages of large specific surface, easy chemical modification, strong oxidation resistance, good biocompatibility, strong electron transfer ability, and optical properties depend on their size and shape. The...

AI Technical Summary

Problems solved by technology

Utilize the characteristics of hollow gold nanoparticles, and the target molecule and complementary DNA bound to the nucleic acid aptamer can compete with the nucleic acid aptamer, or two DNA sequences with partially identical fragments can compete with the complementary DNA strand for binding properties, design and develop new detection reagents and methods for rapid and sensitive detection of target molecules, and there are no relevant reports so far

Images