Specific metal nanowire and production method thereof

Abstract

The invention provides a specific metal nanowire. The specific metal nanowire comprises a linear nanowire matrix, an isolating layer and a binding body, wherein the binding body is used for being coupled with a single DNA molecule, the isolating layer coats the outside surface of the nanowire matrix, and the top end of the nanowire matrix is connected with the binding body. The invention further provides a method for producing the specific metal nanowire. According to the method, the drawbacks that most existing single molecule manipulation techniques can only be used for binding a large amount of DNA single strands but cannot be used for capturing single DNA in a DNA sequencing process and a technique which can be used for controlling a single DNA single strand to pass through a nanoporerequires perforation and is high in production cost are overcome, a target of controlling a single DNA single strand to pass through a nanopore is achieved, a perforation step is omitted, facility requirements are reduced, and the production cost is low.

Description

technical field [0001] The invention relates to the technical field of biomolecular sequencing and micro-nano manufacturing, and more specifically, to a specific metal nanowire and a manufacturing method thereof. Background technique [0002] The fourth-generation nanopore sequencing technology achieves the purpose of sequencing by detecting the potential change of single-stranded DNA when it passes through the nanopore. With its advantages of high throughput and low cost, it has gradually become a research hotspot among scholars at home and abroad. The technology is affected by the thermal motion of DNA and the complex fluid motion in the nanopore (Li J, Gershow M, Stein D, et al.DNAmolecules and configurations in a solid-state nanopore microscope[J].Naturematerials,2003,2(9 ):611.), which makes it very difficult to detect the current signal when DNA passes through the hole. Therefore, in order to realize the possibility of solid-state nanopore sequencing, it is very import...

AI Technical Summary

Problems solved by technology

[0006]In order to overcome the disadvantages of most existing single-molecule manipulation technologies that can only bind a large number of DNA single strands and cannot capture a single DNA during the DNA sequencing process, and The technology that can control a single DNA single strand to pass through a nanopore needs to be drilled and has the disadvantages of high production costs. The present invention provides a specific metal nanowire and a manufacturing method thereof, which are used to operate DNA single strand molecules to pass through a nanopore, and The step of punching holes is avoided, the requirements for equipment are reduced and the production cost is low

Images