Hafnia alvei preparation for disposing of domestic sludge, preparation method and application thereof
A hive Hafnia and domestic sludge technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, biological sludge treatment, etc., can solve the problem of limiting the development of thermal drying technology, large one-time investment, and operating costs Advanced problems, to achieve the effect of simple and easy operation, short processing cycle and low processing cost
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Embodiment 1
[0034] A kind of preparation method of the Hafnia alveoli preparation that is used for processing domestic sludge provided by the preferred embodiment of the present invention, the specific steps are as follows:
[0035] S1. Soak fresh vermicompost in water for 30 minutes, take the soaking solution, the ratio of vermicompost to water is 0.38g: 1mL;
[0036] S2. The soaking solution obtained in the S1 step, the Hafnia alvei bacterial classification and the culture medium are added to the culture container according to the volume ratio of the medium, the soaking liquid and the Hafnia alveoli bacterial classification being 5:0.1:0.01, Cultured at 12°C for 12 hours to obtain the culture mother solution;
[0037] S3. Disperse the culture mother liquor obtained in step S2 in the culture bucket, and then add the culture medium into the culture bucket, the volume ratio of the culture medium and the culture mother liquor is 10:0.2, and cultivate at room temperature for 12 hours to obta...
Embodiment 2
[0040] A kind of preparation method of the Hafnia alveoli preparation that is used for processing domestic sludge provided by the preferred embodiment of the present invention, the specific steps are as follows:
[0041]S1. Soak fresh vermicompost in water for 30 minutes, take the soaking solution, the ratio of vermicompost to water is 0.50g: 1mL;
[0042] S2. The soaking solution obtained in the S1 step and the Hafnia alvei bacterial classification and the culture medium are added to the culture container according to the volume ratio of the medium, the soaking liquid and the Hafnia alveoli bacterial classification being 5.2:0.1:0.01, Cultured at 13°C for 12 hours to obtain the culture mother solution;
[0043] S3. Disperse the culture mother liquor obtained in step S2 in the culture bucket, and then add culture medium into the culture bucket. The volume ratio of the culture medium to the culture mother liquor is 10.2:0.2, and cultivate at room temperature for 12 hours to obt...
Embodiment 3
[0046] A kind of preparation method of the Hafnia alveoli preparation that is used for processing domestic sludge provided by the preferred embodiment of the present invention, the specific steps are as follows:
[0047] S1. Soak fresh vermicompost in water for 30 minutes, take the soaking solution, the ratio of vermicompost to water is 0.45g: 1mL;
[0048] S2. The soaking solution obtained in the S1 step, the Hafnia alvei bacterial classification and the culture medium are added to the culture container according to the volume ratio of the medium, the soaking liquid and the Hafnia alveoli bacterial classification being 5.5:0.1:0.01, Cultured at 15°C for 12 hours to obtain the culture mother liquor;
[0049] S3. Disperse the culture mother liquor obtained in step S2 in the culture bucket, and then add culture medium into the culture bucket. The volume ratio of the culture medium and the culture mother liquor is 10.5:0.2, and cultivate at room temperature for 12 hours to obtain...
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