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New Luteimonas sp species as well as culture method and application thereof

A technology of luteus bacterium and culture medium, which is applied in the field of microbial engineering, can solve the problems of long fermentation cycle, polluted environment, and products that cannot meet food-grade requirements, and achieve the effect of easy cultivation

Inactive Publication Date: 2019-12-20
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method relies on a strong acid environment to hydrolyze chitin. Although the yield is high, there are problems such as environmental pollution and high energy consumption, and its products cannot meet the requirements of food grade; the fermentation method uses microorganisms to synthesize N-acetylamino from various raw materials. Glucose has problems such as long fermentation period and high cost, and it has not been used on a large scale at present

Method used

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  • New Luteimonas sp species as well as culture method and application thereof
  • New Luteimonas sp species as well as culture method and application thereof
  • New Luteimonas sp species as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Isolation and preparation of new bacterial strain YD-1 of the present invention

[0027] Take the soil extract from a vegetable field in Wenzhou City, Zhejiang Province, dilute it and spread it on LB solid medium, culture it at 30°C for 2-3 days, pick a single colony, and then purify it by streaking.

[0028] LB medium components are: peptone 10g; yeast extract powder 5g; NaCl 10g, distilled water to 1000mL. Add agar to solid media.

[0029] The obtained purified strain is preserved, and its preservation number is KCTC 72425.

Embodiment 2

[0030] The appearance characteristics of embodiment 2 new bacterial strain YD-1 of the present invention

[0031] 1. Colony characteristics

[0032] Take a single colony of strain YD-1, transfer it to LB solid medium, and culture it in a constant temperature incubator at 30°C for 24h, 36h, and 48h, and observe the size, color, edge, protrusion, smoothness, and viscosity of the colony respectively , transparency and other characteristics. The results showed that strain YD-1 formed neat, slightly raised, smooth, opaque, yellow colonies with a diameter of about 2-3mm on LB solid medium.

[0033] 2. Cell Morphological Characteristics

[0034] The cells of strain YD-1 are Gram-negative bacilli with blunt apex and no flagella; the cell size is 0.9-1.1 μm×2.6-4.7 μm, solitary.

Embodiment 3

[0035] The growth characteristics of embodiment 3 new bacterial strain YD-1 of the present invention

[0036] Pick fresh cultures cultured on R2A solid medium for 24 hours, inoculate them into LB liquid medium, and cultivate them on a shaker at 30°C for 20-24 hours as seeds.

[0037] Growth temperature:

[0038] The cultured YD-1 seeds were transferred to fresh sterile inoculated LB liquid medium according to 2% (v / v) inoculum amount, and mixed well. They were cultured in water baths at 4°C, 10°C, 15°C, 20°C, 25°C, 30°C, 37°C, 40°C, 45°C, 50°C and 60°C respectively. The growth was measured at 24h and 48h. The growth temperature range of strain YD-1 is 20~50℃, and the optimum temperature is 30℃.

[0039] Growth NaCl tolerance

[0040] The cultured YD-1 seeds were transferred to LB with sodium chloride concentrations of 0.0%, 2.0%, 5.0%, 7.0%, 9%, 10.0%, 11.0% and 12.0% according to the inoculation amount of 2% (v / v). The culture medium was cultured at 30°C, and the growth ...

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Abstract

The invention relates to new Luteimonas sp species as well as a culture method and application thereof. The Luteimonas sp provided by the invention is separated from soil, and the preservation numberis KCTC 72425. The new Luteimonas sp species provided by the invention grow at medium temperature (30 DEG C), are easy to culture, have the function of generating chitinase to degrade chitin, and canprovide a new species resource for producing N-acetylglucosamine by utilizing the chitin which widely exists in the natural world.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a new bacterial strain of Lutemonas luteus and its cultivation method and application. Background technique [0002] The isolation sources of Luteomonas are very extensive, and they are isolated from rhizosphere soil, air, seabed sediments and other environments. In 2000, it was isolated, identified and named for the first time by Finkmann et al. So far, Luteimonas composti, Luteimonas marina, Luteimonas aquatica, Luteimonas aestuarii, Luteimonasterricola, Luteimonas lutimaris and Luteimonas cucumeris have been isolated and identified. [0003] N-acetylglucosamine is a derivative in which the hydroxyl group at the second position of glucose is replaced by an acetylamino group, and it is the smallest unit of chitin. In recent years, N-acetylglucosamine has been found to have the functions of repairing cartilage tissue, relieving joint pain in the treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/26C12R1/01
CPCC12N1/20C12P19/26C12R2001/01C12N1/205
Inventor 周杰陈建豪庄妮莎董维亮薛瑞姜岷
Owner NANJING UNIV OF TECH