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Diagnostic marker circ3823 for early colorectal cancer and application thereof

A diagnostic marker, colorectal cancer technology, applied in the field of tumor markers, can solve the problems of lack, early diagnosis and treatment of CRC, etc., achieve the effect of excellent sensitivity and specificity, reduce the degree of trauma and diagnosis cost

Active Publication Date: 2019-12-20
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that early diagnosis of CRC can significantly improve the 5-year survival rate of patients, but the lack of early specific markers leads to a serious shortage of early diagnosis and treatment of CRC

Method used

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  • Diagnostic marker circ3823 for early colorectal cancer and application thereof
  • Diagnostic marker circ3823 for early colorectal cancer and application thereof
  • Diagnostic marker circ3823 for early colorectal cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: High-throughput sequencing to screen circRNA related to early colorectal cancer

[0021] This research is based on high-throughput sequencing, and the specific screening process is as follows:

[0022] 1. Sample collection:

[0023] The inventor collected 28 cases of early CRC tissue and 28 cases of normal tissue adjacent to the tumor (5cm away from the cancer tissue) from the Colorectal Surgery Department of the First Affiliated Hospital of Zhengzhou University (sample sampling, aliquoting, and uniform storage conditions for research) At the time of sampling, the patient did not receive any preoperative anti-cancer treatment. After the tissue was separated from the human body, the tissue was washed with saline to remove blood stains, and then quickly transferred to liquid nitrogen for storage. During the experiment, try to avoid repeated freezing and thawing of the tissue, and the experimental data restored the expression of RNA in the body as much as possible. Th...

Embodiment 2

[0033] Example two, verification of differential expression

[0034] 1. Sample collection

[0035] On the basis of Example 1, using the same treatment method, the inventor expanded 28 pairs of tissues to 39 pairs, a total of 39 cases of early CRC tissues and 39 cases of normal tissues adjacent to the tumor. In addition, the inventor collected serum samples from 19 early-stage CRC patients and 19 healthy controls, and collected 2-3ml of whole blood with a red-cap vacuum blood collection tube (without anticoagulant and accelerator), and left it at room temperature for 1 hour. Centrifuge at 3000 rpm for 10 min, collect 1 ml of supernatant, and freeze at -80°C for later use; the above study was approved by the ethics committee and the patient informed consent.

[0036] 2. Trizol method to extract RNA

[0037] According to the manufacturer's instructions, RNAiso Plus (TaKaRa) was used to isolate total RNA from serum and early CRC tissue.

[0038] 2.1 After taking the tissue, use the Trizol...

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Abstract

The invention relates to a diagnostic marker for early colorectal cancer, wherein the diagnostic marker is circular RNA circ3823, and the nucleotide sequence of the diagnostic marker is shown in SEQ ID No.1; and an application of the circular RNA circ3823 in preparing a diagnostic tool for early colorectal cancer, wherein the tool is a kit, and the tool comprises a primer pair for amplifying a nucleic acid sequence for specifically recognizing the circular RNA circ3823. The marker can clearly and clearly characterize the occurrence of early colorectal tumor diseases, the expression of the marker in a plurality of early CRC tissue samples is significantly higher than that in a control tissue samples (normal tissues which are 5cm away from tumor tissues), and the expression of the marker ina plurality of early CRC patient serum samples is significantly higher than that in a control healthy serum sample.

Description

Technical field [0001] The present invention relates to the technical field of tumor markers, in particular to circ3823, a diagnostic marker for early colorectal cancer and its application. Background technique [0002] Colorectal cancer (CRC) is a high-incidence tumor of the digestive system, which is highly malignant and seriously affects the survival of patients. Studies have shown that early diagnosis of CRC can significantly improve the 5-year survival rate of patients, but the lack of early specific markers has led to a serious shortage of early diagnosis and treatment of CRC. Therefore, it is urgent and necessary to find the diagnostic markers of early CRC. [0003] Circular RNA (circRNA) is a special type of non-coding RNA that plays an important regulatory role in diseases. As a tumor biomarker, circRNA has many advantages. CircRNA has a closed and circular structure, which is not easily degraded by exonuclease and is more stable than linear RNA. In addition, circRNA i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12N15/113C12Q2600/158C12Q2600/178C12N2310/532C12N2330/10Y02A50/30
Inventor 孙振强郭雅欣汲翔袁维堂汲振余陈晨范丹丹刘金波赵陆洋邵博
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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