Preparation method and product of oat ACE inhibitory peptide
A technology for inhibiting peptides and oats, applied in the field of preparation of oat ACE inhibitory peptides, can solve the problems of loss of Alcalase enzyme activity, weak ACE inhibition of oat ACE inhibitory peptides, and reduced production efficiency of oat ACE inhibitory peptides, etc. The effect of avoiding loss of enzymatic activity
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Embodiment 1
[0027] 1) Add oat bran to deionized water according to the ratio of material to liquid 1:18g / ml, adjust the pH to 9.5, extract it in an ultrasonic biochemical analyzer for 60 minutes, and then centrifuge at 5000r / min for 10 minutes; take the supernatant to adjust the pH to 4.0, and Centrifuge at 4000r / min for 15min, slowly pour off the supernatant, keep the oat protein precipitate, and store the oat protein precipitate in freeze-dry;
[0028] 2) The oat protein was dissolved in deionized water to make a protein solution with a mass concentration of 5%, added protease, and placed in a constant temperature enzyme reactor for enzymolysis. The proteases used for enzymolysis were Alcalase, Trypsin, Pepsin, Pancreatin, Neutrase, Flavourzyme and Protamex do 7 groups of experiments, and add a group of blank control experiments; in each group of experiments, the enzymatic hydrolysis temperature and pH of the constant temperature enzyme reactor are based on the optimal hydrolysis tempera...
Embodiment 2
[0049] 1) Add oat bran to deionized water according to the ratio of material to liquid 1:18g / ml, adjust the pH to 9.5, extract it in an ultrasonic biochemical analyzer for 60 minutes, and then centrifuge at 5000r / min for 10 minutes; take the supernatant to adjust the pH to 4.0, and Centrifuge at 4000r / min for 15min, slowly pour off the supernatant, keep the oat protein precipitate, and store the oat protein precipitate in freeze-dry;
[0050] 2) The oat protein was dissolved in deionized water to make a protein solution with a mass concentration of 5%, added protease, and placed in a constant temperature enzyme reactor at 85°C for enzymolysis for 3 hours. The protease used in the enzymolysis was Alcalase.
[0051] 3) When enzymatically hydrolyzing oat protein in the enzyme reactor, add activated carbon particles for decolorization for 1 hour. During decolorization, stir the enzyme reactor at 100r / min with a stirrer, and then filter the enzyme solution through a filter membrane....
Embodiment 3
[0053] The product of oat ACE inhibitory peptide is mixed with oat ACE inhibitory peptide and Dendrobium polysaccharide, and the mass ratio of described ACE inhibitory peptide and Dendrobium polysaccharide is 1:1, and the mixture of oat ACE inhibitory peptide and Dendrobium polysaccharide is according to mass ratio 1: 15 is added to vitamin C tablets; using oat ACE inhibitory peptide to lower blood pressure, the antioxidant activity of dendrobium polysaccharides and vitamin C can repair blood vessels, which can effectively treat hypertension and cerebral thrombosis.
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