Production technology of L-isoleucine

A production process, the technology of isoleucine, applied in the direction of microorganisms, preparation of organic compounds, methods based on microorganisms, etc., can solve the problems of poor filtration effect, large amount of activated carbon, and high cost, so as to reduce the amount of waste water, increase production, The effect of reducing dosage

Active Publication Date: 2019-12-24
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional decolorization method of domestic fermentation broth is plate frame + activated carbon decolorization and filtration, but this kind of filtration effect is poor, and the amount of activated carbon is large and the cost is high

Method used

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  • Production technology of L-isoleucine
  • Production technology of L-isoleucine
  • Production technology of L-isoleucine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of production technology of L-isoleucine, it comprises the steps:

[0034] Step 1) Fermentation: Using Corynebacterium glutamicum ATCC14309 as the experimental strain, Corynebacterium glutamicum seed solution (OD 600 The value is 12) According to the inoculation amount of 6%, transfer to a 50L fermenter containing 30L fermentation medium for cultivation, the temperature is 30°C, the cultivation time is 60 hours, and the pH of the medium is controlled by automatically adding 20% ​​ammonia water to 6.8-7.0. Control the dissolved oxygen at 25% by stirring and ventilating, add glucose nutrient solution, and control the residual sugar content in the fermentation broth to not be less than 1g / L;

[0035]During the fermentation process, 0.2 L of H 2 o 2 ; In the time of 48h, add chitosan in fermenter, control the concentration of chitosan to be 40mg / L;

[0036] The fermentation medium component is: glucose 80g / L, (NH 4 ) 2 SO 4 20g / L, corn steep liquor 10g / L, KH ...

Embodiment 2

[0042] A kind of production technology of L-isoleucine, it comprises the steps:

[0043] Step 1) Fermentation: Using Corynebacterium glutamicum ATCC14309 as the experimental strain, Corynebacterium glutamicum seed solution (OD 600 The value is 11) According to the inoculum amount of 7%, it is transferred to a 50L fermenter containing 30L fermentation medium for cultivation, the temperature is 30°C, the cultivation time is 60 hours, and the pH of the medium is controlled by automatically adding 20% ​​ammonia water to 6.8-7.0. Control the dissolved oxygen at 20% by stirring and ventilating, add glucose nutrient solution, and control the residual sugar content in the fermentation broth to not be less than 1g / L;

[0044] During the fermentation process, 0.15 L of H 2 o 2 ; In the time of 48h, add chitosan in fermenter, control the concentration of chitosan to be 40mg / L;

[0045] The fermentation medium component is: glucose 80g / L, (NH 4 ) 2 SO 4 20g / L, corn steep liquor 10g...

Embodiment 3

[0065] 1.H 2 o 2 Effects of addition amount and timing on L-isoleucine production and cell biomass in fermentation broth.

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Abstract

The invention belongs to the technical field of production of L-isoleucine, and discloses a production technology of L-isoleucine. The production technology of the L-isoleucine comprises the followingsteps of step (1) fermentation, step (2) concentration and re-dissolution, step (3) first decoloration, step (4) secondary decoloration and step (5) edulcoration and crystallization. The production technology of the L-isoleucine is simple and feasible, and the yield and purity of the L-isoleucine are improved.

Description

technical field [0001] The invention belongs to the technical field of L-isoleucine production, and in particular relates to a production process of L-isoleucine. Background technique [0002] L-isoleucine, also known as isoleucine, chemical name L-2-amino-3 methylvaleric acid, in 1901, Fischer discovered optical activity in the L-leucine fraction separated from protein hydrolyzate This is the earliest report about L-isoleucine. L-isoleucine is an important part of living organisms and belongs to essential amino acids, because its special structure and function play an important role in human life metabolism. If the human body lacks L-isoleucine for a long time, it will affect the physiological function of the body, leading to metabolic disorders and decreased resistance. With its unique efficacy, L-isoleucine is widely used in food health care, biomedicine, medical beauty and other industries. [0003] The production scale of isoleucine in my country is small, the acid p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/06C07C227/40C07C229/08C12R1/15
CPCC12P13/06C07C227/40C07C229/08Y02P20/10
Inventor 包鑫杨晓芳刘元涛李江雷边恩来张宗华
Owner XINJIANG FUFENG BIOTECH
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