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Primer group, kit and method for evaluating chronic cardiac failure prognosis

A chronic heart failure, primer set technology, applied in the primer set for evaluating the prognosis of chronic heart failure, the field of evaluating the prognosis of chronic heart failure, can solve the problems of the accuracy of the detection method, age and the influence of obesity, and achieve guidance compliance and standardization , to ensure accuracy and comprehensiveness, to ensure the effect of accuracy

Inactive Publication Date: 2019-12-24
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

NT-proBNP detection uses the level of NT-proBNP, a cardiac function marker, to reflect the degree of deterioration of chronic heart failure. Although this detection method has been widely used in the assessment of chronic heart failure, the accuracy of this detection method will be affected by age and obesity. Impact

Method used

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  • Primer group, kit and method for evaluating chronic cardiac failure prognosis
  • Primer group, kit and method for evaluating chronic cardiac failure prognosis
  • Primer group, kit and method for evaluating chronic cardiac failure prognosis

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Embodiment 1

[0030] The embodiment of the present invention provides a primer set for evaluating the prognosis of chronic heart failure, which is suitable for evaluating the prognosis of chronic heart failure in Han patients with primary dilated cardiomyopathy and ischemic dilated cardiomyopathy. The primer set includes: the first Upstream primer, first downstream primer, second upstream primer, second downstream primer, third upstream primer, third downstream primer, fourth upstream primer, fourth downstream primer, fifth upstream primer, fifth downstream primer, sixth Upstream primer, the sixth downstream primer, the seventh upstream primer, the seventh downstream primer, the eighth upstream primer and the eighth downstream primer, the sequence of the first upstream primer is as shown in SEQ ID NO:1 in the sequence listing, the first downstream primer The sequence of the primer is as shown in SEQ ID NO:2 in the sequence listing, the sequence of the second upstream primer is as shown in SE...

Embodiment 2

[0036] An embodiment of the present invention provides a kit for evaluating the prognosis of chronic heart failure, the kit includes: the primer set provided in Embodiment 1.

[0037] Specifically, the kit also includes: TaKaRa Ex Taq HS, 10×Ex Taq, dNTP Mixture, PrimerPool 1 / 2, genomic DNA and sterilized water.

[0038] The embodiment of the present invention provides a kit for evaluating the prognosis of chronic heart failure. The kit can accurately amplify the rs679899 SNP site corresponding to the APOB gene, the rs1042464 SNP site corresponding to the HRG gene, and the TLN2 gene in the sample to be tested. The corresponding rs1320191 SNP site, the rs2301629 SNP site corresponding to the SLC25A13 gene, the rs2536512 SNP site corresponding to the SOD3 gene, the rs3134587 SNP site corresponding to the SYNM gene, the rs4273214 SNP site corresponding to the AGXT gene, and the rs33958047 SNP site corresponding to the AGXT gene point, eight amplification products are obtained, an...

Embodiment 3

[0040] An embodiment of the present invention provides a method for evaluating the prognosis of chronic heart failure using the primer set provided in Embodiment 1, the method comprising:

[0041] Patient Xiang, aged 44, male, was diagnosed with dilated cardiomyopathy, coronary atherosclerosis, had a history of arrhythmia, denied any history of diabetes or hypertension, had no smoking or drinking habits, was 175cm tall, weighed 53kg, and had a blood pressure of 123 / 81mmHg, heart rate 88 beats / min, NYHA class 4, fasting blood glucose 4.66(mmol / L), ALT 55(U / L), AST36(U / L), TC 4.11(mmol / L), TG1.08(mmol / L), Cr 76(umol / L), HDL 1.49(mmol / L), LDL2.31(mmol / L), NT-proBNP 2650ng / L, left ventricular ejection fraction 25 %, the left internal diameter is 77cm, the left atrium internal diameter is 45cm, the interventricular septum is 9cm, and the left ventricular posterior wall is 8cm. The patient took metoprolol tartrate tablets, digoxin, furosemide tablets, spironolactone tablets and ca...

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Abstract

The invention discloses a primer group, kit and method for evaluating chronic cardiac failure prognosis, and belongs to the field of gene detection. The primer group comprises a first upstream primer,a first downstream primer, a second upstream primer, a second downstream primer, a third upstream primer, a third downstream primer, a fourth upstream primer, a fourth downstream primer, a fifth upstream primer, a fifth downstream primer, a sixth upstream primer, a sixth downstream primer, a seventh upstream primer, a seventh downstream primer, an eighth upstream primer and an eighth downstream primer. Genes amplified by the primer group correspond to eight risk alleles sensitive to prognosis correspondingly, and accuracy and comprehensiveness of the evaluated result can be ensured; and according to the method, by conducting sequencing and data interpretation on a target amplification region, mutations relevant to prognosis can be accurately identified, thus the variety and pathogenesis of diseases are judged, and a timely and reliable detection report is provided for clinic.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a primer set, a kit and a method for evaluating the prognosis of chronic heart failure. Background technique [0002] Chronic heart failure is the terminal state of cardiovascular disease resulting from cardiac ejection fraction not meeting the body's needs. The main clinical symptoms are chronic fatigue, shortness of breath, dyspnea, pulmonary congestion and edema. In the general population, women are more affected than men, with a prevalence rate of 9 / 1000. At present, there are about 4 million patients with chronic heart failure in my country. Although there are currently new drugs and auxiliary facilities for the treatment of chronic heart failure, the prognosis of chronic heart failure is poor, and the five-year survival rate is comparable to that of malignant tumors. Therefore, early prognosis assessment and active treatment based on the assessment results are extremely import...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/118C12Q2600/156C12Q2531/113C12Q2535/101
Inventor 汪道文孙阳李诗洋
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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