Composition comprising salvia miltiorrhiza extract for prevention, alleviation, or treatment of visceral fat obesity
A technology of visceral fat and composition, which is applied in the direction of drug combination, medical preparations containing active ingredients, food science, etc., to improve or treat visceral fat-type obesity and achieve the effect of inhibiting efficacy
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Embodiment 1
[0051] Embodiment 1. manufacture salvia miltiorrhiza extract
[0052] The method for extracting from Danshen includes: a) extracting with water or an organic solvent to obtain a crude extract; b) concentrating (under reduced pressure) after filtering the crude extract; c) concentrating and drying the extract to prepare a polysaccharide Steps for a dosage form.
[0053] (1) Steps of extracting salvia miltiorrhiza
[0054] A step of obtaining Danshen extract by using hot water or 20-100% by weight ethanol. According to the design conditions, other solvents can be used. According to the type of extraction solvent, the extraction temperature, extraction time, amount of solvent and residual component treatment method can be designed differently. A variety of solvents can be used as the extraction solvent, and the extractable solvents include water, ethanol, methanol, fatty oil, glycerin, horse oil, ethyl acetate, acetone, butanol, isopropanol, and methylene chloride.
[0055] (2...
experiment example 1
[0061] Experimental Example 1. Analysis of Salvia Miltiorrhiza Extract
[0062] Nuclear magnetic resonance (NMR) analysis was performed to determine the structures of cryptotanshinone, tanshinone I, tanshinone IIA, and 15,16-dihydrotanshinone I, which are active ingredients of the Danshen extract extracted in Example 1. In addition to the above-mentioned four kinds of tanshinones, the salvia miltiorrhiza extract contains other tanshinones such as salvianolic acid, tanshinone IIB, hydrogenated tanshinone, and tanshinoic acid.
[0063] Cryptotanshinone
[0064] 1H-NMR (CDCl3): δ7.42 (2H, ABq, J = 8.0Hz), 4.83 (1H, t, J = 9.2Hz), 4.31 (1H, dd, J = 9.2and 6.0Hz), 3.55 (1H ,m),3.17(2H,br t),1.65(4H,m),1.40(3H,d,J=6.8Hz),1.28(6H,s)13C-NMR(CDCl3):δ9.58(C- 1), 19.00(C-2), 37.73(C-3), 34.76(C-4), 143.57(C-5), 132.48(C-6), 122.43(C-7), 128.30(C-8 ), 126.19(C-9), 152.28(C-10), 184.16(C-11), 175.59(C-12), 118.21(C-13), 170.66(C-14), 81.38(C-15) ,34.54(C-16),18.74(C-17),31.85(C-18),3...
experiment example 2
[0071] Experimental Example 2. Visceral Fat Reduction Efficacy of Danshen Extract in Genetic Obesity Model Animal C57BL / 6JL Lep ob / Lep ob
[0072] For genetic obesity model animals C57BL / 6JL Lep ob / Lep ob eight-week-old mice, set the indoor temperature to an average of 20°C±2°C, maintain a relative humidity of 60%±10%, and maintain light / darkness in a 12-hour cycle The environmental conditions of the animal breeding room. Animals were housed in well-ventilated plastic cages. Feed (purina) and litter were changed and provided periodically, and water was freely available for drinking. Before starting the animal experiments, experiments were performed at 8 weeks of age after a one-week acclimatization period as a period for adaptation to the environmental conditions of the breeding room.
[0073] After the acclimatization process, the Danshen extract was administered at 100-1000 mg / kg. 0.47% (corresponding to 400 mg / kg) and 0.7% (corresponding to 600 mg / kg) were mixed and pell...
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