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Nucleic acid detection chip for portable equipment and use method thereof

A portable device and detection chip technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, bioreactor/fermenter for specific purposes, etc., can solve the problem of increasing the complexity of the chip manufacturing process and the complexity of the external control system , Difficulty in mass production, etc., to avoid aerosol pollution of amplification products, avoid sample cross-contamination, and simplify the design

Active Publication Date: 2019-12-27
INTEGRATED BIOSYSTEMS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of microfluidic chip products that integrate nucleic acid purification and amplification detection in the market. The existing microfluidic chip research is mainly concentrated in the field of scientific research, with a high degree of integration. A large number of microvalve or precision structures are designed on the chip, which increases the The complexity of the chip manufacturing process, the difficulty of mass production, the high cost, and the complexity of the external control system

Method used

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  • Nucleic acid detection chip for portable equipment and use method thereof
  • Nucleic acid detection chip for portable equipment and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Such as figure 1 As shown, the nucleic acid detection chip for portable devices disclosed by the present invention consists of two parts: an outer functional area and an inner functional area. Wherein, the outer functional area is circular, and its position can be fixed by the limiting groove (9) in the outer functional area, and the outer functional area includes a purification reagent storage unit, a waste liquid unit and an amplification detection unit.

[0039] Purification reagent storage unit sequentially includes pretreatment liquid chamber (1), sample chamber (2), washing liquid chamber I (3), washing liquid chamber II (4), dealcohol liquid chamber (5), eluent chamber (6 ), and each liquid storage chamber contains a vent hole (7) for injecting reagents or ventilating with the outside world.

[0040] The liquid storage chamber of the purification reagent storage unit is sequentially filled with nucleic acid purification reagents, the pretreatment liquid chamber ...

Embodiment 2

[0058] The structure of the nucleic acid detection chip used in the portable device is the same as that in Example 1. The chip is used as follows:

[0059] 1) Add an equal amount of sample into the treatment solution, mix well and inject into the sample chamber of the above chip (2);

[0060] 2) Place the chip on the supporting portable device; initialize the device, connect the pretreatment liquid chamber (1) and the waste liquid chamber (10) in the inner functional area, and infiltrate the purification chamber (21) of the nucleic acid extraction unit with the pretreatment liquid and the corresponding pipeline, and redissolve the dry powdered superparamagnetic nano-magnetic beads, and after the external magnetic field is turned on to absorb the magnetic beads, the pretreatment liquid is sucked into the waste liquid chamber (10);

[0061] 3) The functional area inside the motor rotation is connected to the sample chamber (2), the purification chamber (21) and the waste liquid...

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Abstract

The invention discloses a nucleic acid detection chip for portable equipment and a use method thereof. The chip can realize automatic extraction, amplification and detection processes of sample nucleic acids. The chip does not require a complex structure and micro-valves, the cost and difficulty of mass production are greatly reduced, and complexity of an external control system is reduced. Nucleic acid purification, amplification, and detection reagents are pre-loaded inside the chip, the chip is stored at room temperature and adopts closed operation, and thereby cross-contamination of samples and aerosol contamination of amplification products can be avoided.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction and amplification detection, in particular to a microfluidic chip and a method for using the same. Background technique [0002] Nucleic acid is the most basic genetic material of life and widely exists in all animals, plants, and microbial cells. With the rapid development of nucleic acid analysis technology, more and more disciplines regard the purification, detection and analysis of nucleic acid as the main technical means. For example, the prevention, control and diagnosis and treatment of infectious diseases, the identification of bioterrorism factors, food-borne pathogenic factors and mutant gene components, port sanitation and pest defense quarantine. Commonly used nucleic acid extraction and purification methods include phenol-chloroform extraction, spin column method, and magnetic bead method. The extraction process is complex, cumbersome, time-consuming, and may use tox...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/6837C12Q1/6806C12Q2563/155C12Q2563/143C12Q2563/149
Inventor 蔡亦梅高静范东雨李洁昆张瑜代有来任鲁风
Owner INTEGRATED BIOSYSTEMS CO LTD