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Tussah silk fibroin hydrogel as well as preparation method and application thereof

A technology of tussah silk fibroin and hydrogel, which is applied in the field of tussah silk fibroin hydrogel and its preparation, and can solve problems such as fragility, poor mechanical properties, and strong hydrophobicity

Active Publication Date: 2019-12-31
EASTERN LIAONING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, tussah silk fibroin contains a large number of polyalanine sequences and is highly hydrophobic. After forming a gel, its mechanical properties are poor and fragile, which cannot meet the needs of modern biological materials.

Method used

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  • Tussah silk fibroin hydrogel as well as preparation method and application thereof
  • Tussah silk fibroin hydrogel as well as preparation method and application thereof
  • Tussah silk fibroin hydrogel as well as preparation method and application thereof

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preparation example Construction

[0028] The invention provides a preparation method of tussah silk fibroin hydrogel, comprising the following steps:

[0029] (1) Mix tussah silk fibroin with a quaternary ammonium salt to undergo a ring-opening addition reaction to obtain quaternized tussah silk fibroin;

[0030] (2) Mixing the quaternized tussah fibroin protein with N-isopropylacrylamide, and under the action of an initiator, an in-situ polymerization reaction occurs to obtain the tussah silk fibroin hydrogel.

[0031] In the present invention, unless otherwise specified, the preparation materials used are commercially available products well known to those skilled in the art.

[0032] The invention mixes the tussah silk fibroin with the quaternary ammonium salt to undergo a ring-opening addition reaction to obtain the quaternized tussah silk fibroin. The present invention has no special limitation on the preparation method of the tussah silk fibroin protein, and the conventional tussah silk fibroin protein ...

Embodiment 1

[0056] Put 1.543g of tussah silk fibroin protein into a three-necked flask, add deionized water to dissolve, and prepare a 25mg / mL tussah silk fibroin protein aqueous solution, and adjust the tussah silk fibroin protein aqueous solution to a pH of 10. Immerse the above-mentioned three-neck flask in an ice-water bath, and keep the temperature at 5°C; then dissolve 3.086g of 2,3-epoxypropyltrimethylammonium chloride in 10mL of deionized water, and use constant pressure under magnetic stirring. Add the 2,3-epoxypropyltrimethylammonium chloride solution dropwise into the three-necked flask through the dropping funnel at a rate of 1 drop / 5 seconds. After the addition, adjust the temperature of the water bath to 20°C and open the ring. Addition reaction for 2 hours; place the obtained solution in a 8000-14000 kDa dialysis bag, and dialyze in a refrigerator at 4°C for 24 hours, during which the water is changed every 4 hours to remove unreacted quaternary ammonium salt; the dialyzed s...

Embodiment 2

[0059] Put 1.809g of tussah silk fibroin protein into a three-necked flask, add deionized water to dissolve, and prepare a 25mg / mL tussah silk fibroin protein aqueous solution, and adjust the tussah silk fibroin protein aqueous solution to a pH of 10. Submerge the above-mentioned three-neck flask in an ice-water bath, and keep the temperature at 5°C; then dissolve 4.523g of 2,3-epoxypropyltrimethylammonium chloride in 10mL of deionized water, and use constant pressure under magnetic stirring. Add the 2,3-epoxypropyltrimethylammonium chloride solution dropwise into the three-necked flask through the dropping funnel at a rate of 1 drop / 5 seconds. After the addition, adjust the temperature of the water bath to 25°C and open the ring. Addition reaction for 2.5h; put the obtained solution in a 8000-14000kDa dialysis bag, dialyze in a 4°C refrigerator for 24h, and change the water every 4h during this period to remove unreacted quaternary ammonium salt; freeze-dry the dialyzed soluti...

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Abstract

The invention relates to the technical field of biomedical materials, and in particular, relates to tussah silk fibroin hydrogel as well as a preparation method and an application thereof. The invention provides the preparation method of the tussah silk fibroin hydrogel, wherein the preparation method comprises the following steps: (1) mixing tussah silk fibroin with quaternary ammonium salt, andcarrying out ring-opening addition reaction to obtain quaternized tussah silk fibroin; and (2) mixing the quaternized tussah silk fibroin with N-isopropylacrylamide, and carrying out an in-situ polymerization reaction under the action of an initiator to obtain the tussah silk fibroin hydrogel. The tussah silk fibroin hydrogel prepared by the preparation method disclosed by the invention is good inbiocompatibility and strong in cell adhesion capability, can promote cell proliferation, and has good mechanical properties, temperature responsiveness and antibacterial property.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a tussah silk fibroin hydrogel and a preparation method and application thereof. Background technique [0002] Biomedical materials need to have the following characteristics: (1) biocompatibility that matches biological tissues and promotes tissue cell proliferation; (2) mechanical strength that matches biological tissues; (3) degradation products have no toxic and side effects; (4) have Adjustable functionality. [0003] At present, silk fibroin has been widely used in tissue engineering, biomedical and cell engineering matrix materials. It is non-toxic, non-irritating, low or non-immunogenic, has good biocompatibility, and can promote cell growth. Silk fibroin is the general term for domestic silk fibroin and wild silk fibroin. In recent decades, domestic silk fibroin has been continuously developed and has been widely used, but wild silk fibroin has not been ful...

Claims

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Application Information

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IPC IPC(8): C08F289/00C08F220/54C08J3/075C08H1/00C12N5/00A61K9/06A61K47/42A61L15/32A61L15/42A61L15/46A61L27/22A61L27/52A61L27/54A61P31/04
CPCA61K9/06A61K47/42A61L15/32A61L15/42A61L15/46A61L27/227A61L27/52A61L27/54A61L2300/252A61L2300/404A61L2430/06A61P31/04C08F289/00C08H1/00C08J3/075C08J2351/00C12N5/0068C12N2533/30C12N2533/50C08L89/00C08F220/54
Inventor 王勃翔路艳华李佳程德红郝旭
Owner EASTERN LIAONING UNIV
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