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Application of CMDL-1, kit for diagnosis of heart disease and drug for treating heart disease

A CMDL-1, 1. CMDL-1 technology, applied in the field of biomedical engineering, can solve problems such as improving disease prognosis

Active Publication Date: 2019-12-31
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When congestive heart failure occurs, the mortality rate is approximately 50% and there are currently no treatments available to improve the prognosis of the disease

Method used

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  • Application of CMDL-1, kit for diagnosis of heart disease and drug for treating heart disease
  • Application of CMDL-1, kit for diagnosis of heart disease and drug for treating heart disease
  • Application of CMDL-1, kit for diagnosis of heart disease and drug for treating heart disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1 Changes in the level of CMDL-1 expression changes under the stimulation of doxorubicin (Dox)

[0086] 1.H 9 C 2 cardiomyocyte culture

[0087] h 9 C 2 The cells were derived from rat cardiomyocytes and were treated with DMEM containing 10% serum, 1% penicillin and streptomycin in 5% CO 2 in a cell culture incubator at 37°C.

[0088] 2. Dox treatment of cardiomyocytes

[0089] After being cultured with Dox, the total RNA of the cells was extracted, and the expression level of CMDL-1 was detected by real-time fluorescence quantitative PCR technique.

[0090] Specifically, the total RNA was extracted with Trizol reagent, and after being treated with DNase I (Takara, Japan), the RNA was reverse-transcribed into cDNA by reverse transcriptase. Quantitative real-time PCR was performed on a real-time PCR detection system (CFX96, Bio-Rad). The qRT-PCR system included 12.5 μl 2×SYBR Green (Takara, Japan), 1 μl primer (R / F), 1 μl cDNA, and added water to a total ...

Embodiment 2

[0091] Example 2 CMDL-1 can inhibit the mitochondrial division induced by doxorubicin (Dox) at the cellular level

[0092] 1.H 9 C 2 cardiomyocyte culture

[0093] h 9 C 2 The cells were derived from rat cardiomyocytes and were treated with DMEM containing 10% serum, 1% penicillin and streptomycin in 5% CO 2 in a cell culture incubator at 37°C.

[0094] 2. to H 9 C 2 Cardiomyocytes were transfected with CMDL-1 overexpression vector.

[0095] Specifically: prepare a density of 3 to 5 × 10 in the complete medium 4 H 9 C 2 For the cell suspension, appropriate cells are inoculated into a culture plate, and cultured in a cell incubator with 5% carbon dioxide for 16-24 hours until the confluence of the cells is 20-30%. According to the multiplicity of infection of the cells and the virus titer, add the corresponding amount of virus, the calculation formula: virus volume=(multiplicity of infection×cell number) / virus titer, after cultivating in a cell incubator with 5% carb...

Embodiment 3

[0099] Example 3 CMDL-1 can inhibit the apoptosis induced by doxorubicin (Dox) at the cellular level

[0100] 1.H 9 C 2 cardiomyocyte culture

[0101] h 9 C 2 The cells were derived from rat cardiomyocytes and were treated with DMEM containing 10% serum, 1% penicillin and streptomycin in 5% CO 2 in a cell culture incubator at 37°C.

[0102] 2. to H 9 C 2 Cardiomyocytes were transfected with CMDL-1 overexpression vector.

[0103] Specifically: prepare a density of 3 to 5 × 10 in the complete medium 4 H 9 C 2 For the cell suspension, appropriate cells are inoculated into a culture plate, and cultured in a cell incubator with 5% carbon dioxide for 16-24 hours until the confluence of the cells is 20-30%. According to the multiplicity of infection of the cells and the virus titer, add the corresponding amount of virus, the calculation formula: virus volume=(multiplicity of infection×cell number) / virus titer, after cultivating in a cell incubator with 5% carbon dioxide fo...

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Abstract

The invention provides an application of CMDL-1, a kit for diagnosis of the heart disease and a drug for treating the heart disease, and relates to the technical field of biomedical engineering. The cDNA sequence of long-chain non-coding RNA CMDL-1 related with the heart disease is represented as SEQ ID NO.1. By means of the experiment, it is discovered that expression of CMDL-1 in myocardial cells stimulated by adriamycin is obviously down-regulated, and the purpose of diagnosis of the heart disease can be achieved by specific detection for CMDL-1. Meanwhile, by means of overexpression of CMDL-1, chondriokinesis of the myocardial cells induced by adriamycin can be effectively inhibited, and furthermore, apoptosis of the myocardial cells due to adriamycin induction is effectively reduced.CMDL-1 is expected to provide significant biological indicators for clinical diagnosis and treatment of the heart disease, and new effect targets and new ideas are provided for heart disease resistantdesign.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to an application of CMDL-1, a kit for diagnosing heart disease and a medicine for treating heart disease. Background technique [0002] The anthracycline anticancer drug doxorubicin is an effective and commonly used chemotherapy drug for various malignancies. Use is limited due to cardiotoxic side effects. Doxorubicin-induced cardiomyopathy is a fatal disease, which may be acute and usually occurs within 2-3 days after administration, with an incidence of about 11%. Acute left ventricular (LV) failure is a rare manifestation of acute cardiotoxicity and is reversible with appropriate treatment. The incidence of chronic cardiotoxicity caused by doxorubicin is much lower, the incidence rate is about 1.7%, and it usually occurs around 30 days after administration, and may also occur within 6-10 years after treatment. The incidence of doxorubicin-induced cardiomyopathy...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G01N33/68A61K38/17A61P9/00A61P9/10A61P9/04
CPCC12Q1/6883G01N33/6893A61P9/00A61P9/10A61P9/04C12Q2600/158G01N2333/47G01N2800/324G01N2800/325A61K38/00
Inventor 李培峰曾焕玉陈夏添
Owner QINGDAO UNIV
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