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Molecular imprinted polymers for chemosensing

A technology of molecular imprinting and polymer, applied in the field of detection of analytes in water, can solve the problem of low cost

Inactive Publication Date: 2019-12-31
NAT UNIV OF SINGAPORE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the costs related to the instruments used and / or time to use complex instruments must be kept low to ensure cost-effectiveness

Method used

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  • Molecular imprinted polymers for chemosensing
  • Molecular imprinted polymers for chemosensing
  • Molecular imprinted polymers for chemosensing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0163] Synthesis of MIP-GSMS and MIP-MIBS using alternative templates of choice

[0164] Alternative template selection

[0165] Although both GSM and 2-MIB are commercially available, both compounds are too expensive to be used as templates in the synthesis of their respective MIPs. This is not practical when large amounts of these compounds are required to synthesize MIPs for large-scale applications. Therefore, the Maybridge, ChemBridge and Asinex databases were screened against shape queries (GSM and 2-MIB) using shape-based screening tools, where shape similarity search methods identify similar compounds based on their shape as well as their atomic type. All calculations use software( Release 2015-1 Maestro, version 10.1, LLC, New York, 2015). The main criteria for selecting alternative templates for MIP synthesis were based on their commercial availability, cost and having a high shape similarity score. From these screens, respective surrogate templates for GS...

Embodiment 2

[0181] Example 2: Determination of binding efficiency and binding capacity of MIP-GSMS and MIP-MIBS

[0182] Binding experiments of MIP to GSM and 2-MIB were performed in triplicate batches using the method presented below to investigate the recognition performance of MIP in aqueous solution.

[0183] Kinetic studies to determine optimal incubation times

[0184] By mixing 15 mg of MIP-GSMS / MAA / TRIM2 with 1.37 mmol L -1 GSM or 15 mg of MIP-MIBS / MAA / EDGMA2 with 1.37 mmol L -1 The contact time was studied by equilibrating the 2-MIB solution for 30 min up to a fixed period of 10 h. The mixture was centrifuged and the supernatant was analyzed for GSM or 2-MIB using GC-MS. The binding capacity of MIP to GSM or 2-MIB was calculated and the optimal incubation period was determined. It was observed that both MIP-GSMS / MAA / TRIM2 and MIP-MIBS / MAA / EDGMA2 reached binding equilibrium after 1 h (respectively image 3 a and image 3 b). Therefore, the optimal binding duration was det...

Embodiment 3

[0196] Example 3: Characterization of MIP-GSMS / MAA / TRIM2 and NIP-MAA / TRIM2 using Field Emission Scanning Electron Microscopy (FESEM)

[0197] The polymer samples were coated with a thin gold film and then analyzed by FESEM (JEOL JSM-6700F) at 5.0 kV. The morphology of MIP-GSMS / MAA / TRIM2 at different magnifications is as follows Figure 7 a-c shown. Compared with the morphology of NIP-MAA / TRIM2 ( Figure 7 d), Both MIP-GSMS / MAA / TRIM2 and NIP-MAA / TRIM2 appear as uniform spherical particles. In addition, the size of MIP-GSMS / MAA / TRIM2 is almost the same as that of NIP-MAA / TRIM2, which is 2 μm in diameter.

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Abstract

Disclosed herein is a method of manufacturing molecularly imprinted polymers for scarce target molecules that are made using surrogate molecules. Also disclosed herein are the molecularly imprinted polymers and their use in detecting the selected target molecules, particularly through the binding of a fluorescent surrogate molecule to the molecularly imprinted polymers that is then displaced fromthe molecularly imprinted polymer upon contact with the target molecule. In a preferred embodiment, the molecularly imprinted polymer is formed from a functional monomer selected from methyl methacrylate, a crosslinking agent selected from trimethylolpropane trimethacrylate or ethylene glycol dimethacrylate and the target molecule is selected from geosmin and 2-methylisoborneol. The surrogate molecule is selected through a shape-based screening tool wherein the surrogate has a shape similarity score of at least 0.8 and the ratio of the surrogate molecule to the functional monomer is from 1: 2to 1: 6 and the ratio of the functional monomer to cross-linking agent is 1: 1 to 1: 2.5. The molecularly imprinted polymers are used for the detection of analytes in water.

Description

technical field [0001] The present invention relates to molecularly imprinted polymers bound to complementary fluorescent labels, and the use of such materials for the detection of analytes in water. Background technique [0002] The listing or discussion of a prior-published document in this specification should not necessarily be taken as an admission that the document is part of the state of the art or is common general knowledge. [0003] Global algal blooms from toxic phytoplankton species have increased in frequency and distribution in recent years. Phytotoxins produced by harmful algal blooms (HABs), especially dinoflagellate, can poison marine life and humans when they exceed a certain threshold. There is also concern that drinking water may also be contaminated with these metabolites (D. Caron, et al., Water Research, 2010, 44, 385-416). [0004] In developed countries, consumers' assessment of drinking water quality goes beyond regulatory requirements for health-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/18B01J20/285C40B30/04C08F220/06
CPCB01J20/267B01J20/268G01N33/18G01N2021/6439C08F220/06C08F220/325C08F220/14C08F220/56C08F2810/20G01N21/6428
Inventor 蔡丽玲唐彦华龙籍耀李程倪馼鸿
Owner NAT UNIV OF SINGAPORE