Special purification column for aflatoxin M group and application

An aflatoxin and purification column technology, applied in chemical instruments and methods, separation methods, instruments, etc., can solve the problems of unsatisfactory adsorption effect, increased detection cost, complicated operation steps, etc., and achieves easy mass production and low cost. , the effect of the production method is simple

Active Publication Date: 2020-01-03
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immunoaffinity column method has cumbersome operation steps and relatively high technical requirements; moreover, for AFM 1 Designed immunoaffinity columns for AFM 2 The adsorption effect is often not ideal, resulting in AFM 2 The recovery rate is not high; in addition, in terms of cost, AFM 1 The price of a single immunoaffinity column is about 100 yuan, and its validity period is short, which invisibly increases the detection cost

Method used

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  • Special purification column for aflatoxin M group and application
  • Special purification column for aflatoxin M group and application
  • Special purification column for aflatoxin M group and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: the raw material of AFM-P post and preparation

[0063] (1) Raw materials:

[0064] Column tube: the capacity is 3mL, the inner diameter is 1cm, and the length is 5cm; the filling amount of the filler in the column tube is 200mg;

[0065] Sieve plate: polyethylene sieve plate with a pore size of 10 μm;

[0066] Filler: graphitized carbon (PestiCarb) with a particle size of 120 to 400 meshes and nanometer iron tetroxide (Fe 3 o 4 );

[0067] (2) Preparation:

[0068] Install a lower sieve plate at the liquid outlet of the column tube, then mix 150mg of graphitized carbon and 50mg of nanometer iron tetroxide, fill it into the column tube from the liquid inlet end of the column tube, and then install the upper sieve plate into the column tube, among which , the upper sieve plate, the lower sieve plate and the column tube are installed with an interference fit, and then compressed.

Embodiment 2

[0069] Embodiment 2: the raw material of AFM-P column and preparation

[0070] (1) Raw materials: consistent with Example 1;

[0071] (2) Preparation: Install a lower sieve plate at the liquid outlet end of the column tube, then mix 160mg of graphitized carbon and 40mg nanometer ferric oxide, fill it into the column tube from the liquid inlet end of the column tube, and then install the upper sieve plate Into the column tube, wherein, the upper sieve plate, the lower sieve plate and the column tube are installed with interference fit and pressed tightly.

Embodiment 3

[0072] Embodiment 3: the raw material of AFM-P column and preparation

[0073] (1) Raw materials: consistent with Example 1;

[0074] (2) Preparation: Install a lower sieve plate at the liquid outlet end of the column tube, then mix 120mg of graphitized carbon and 60mg nano-iron ferric oxide, fill it into the column tube from the liquid inlet end of the column tube, and then install the upper sieve plate Into the column tube, wherein, the upper sieve plate, the lower sieve plate and the column tube are installed with interference fit and pressed tightly.

[0075] Contrasting the purification columns made in Examples 1 to 3, it is found that the purification column purification effect made in Example 1 is the best, so it can be seen that 150mg PestiCarb and 50mg Fe 3 o 4 Mixing, the AFM-P column made of this combination as solid phase extraction filler can achieve better adsorption effect.

[0076] 2. Comparative example:

[0077] The AFM-P column that embodiment 1 is made ...

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Abstract

The invention belongs to the field of milk and milk product quality safety detection, and particularly discloses a special purification column for an aflatoxin M group and an application. The specialpurification column comprises a column pipe; a lower sieve plate, a filler and an upper sieve plate are sequentially arranged in the column pipe from the liquid outlet end of the column pipe to the liquid inlet end of the column pipe; and the filler is a mixture of graphitized carbon and nano ferroferric oxide. The invention also discloses an application of the special purification column to the purification and detection of the aflatoxin M group in milk and milk products. The special purification column for the aflatoxin M group is simple and convenient to manufacture and low in cost, is applied to the detection of the milk and milk products, can simplify operation and shorten detection time, can specifically adsorb AFM1 and AFM2, and has excellent adsorption effect; impurities in sampleintroduction are greatly reduced after being purified by the special purification column; and the special purification column has extremely strong practical popularization significance.

Description

technical field [0001] The invention belongs to the field of quality and safety detection of milk and dairy products, and in particular relates to a special purification column for aflatoxin M group and its application. Background technique [0002] Aflatoxin M 1 (aflatoxin M 1 , AFM 1 ) is the ingestion of cows contaminated with aflatoxin B 1 (AFB 1 ) The product produced by hydroxylation in the body after feed is often secreted in milk, stable in nature, and hardly destroyed after pasteurization or high temperature sterilization. AFM 1 It can inhibit the synthesis of RNA in liver cells, prevent and affect the synthesis and metabolism of proteins, fats, mitochondria, enzymes, etc., leading to mutation, cancer and liver cell necrosis. In 2002, the International Agency for Research on Cancer listed it as a class I carcinogen. Many countries and regions in the world have strictly regulated AFM 1 The limit standard of AFM in milk and dairy products is stipulated in China...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14B01D15/22
CPCB01D15/22G01N30/02G01N30/14
Inventor 张巧艳陈亭亭王夏君杨胜利张英林琳杨兰花黄娟
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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