Theinvention discloses a preparation method of a nano soil remediation conditioner. The preparation method disclosed by the invention has the beneficial effects that 1, wheat stalks, barley stalks, teosinte and pennisetum hydridum are selected, the yield is high, and the cost is low; meanwhile, a large amount of plant fiber can be provided, and soil nutrients can be effectively fixed; 2, mixing the wheat stalks, the barley stalks, the teosinte and the pennisetum hydridum with EM (Effective Microorganisms) bacteria and barnyard manure, nutrients which can be easily absorbed by plants can be formed by adopting an anaerobic fermentation method, and the yield of crops can be greatly increased; 3, zeolite, pulverized fuel ash, coal cinder and kaolin are mutually matched, so that metal cadmium in soil can be effectively adsorbed, and an effect of reducing thecontent of cadmium in the soil can be further achieved.