Method for detecting valproic acid in blood
A technology of valproic acid and blood, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection efficiency, long time, and long detection time of blood samples, achieve good sample recovery, improve accuracy, The effect of high sensitivity
Inactive Publication Date: 2020-01-03
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Problems solved by technology
The detection time of blood samples is low due to the long time of extraction processing, which makes the detection time long
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[0039] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings.
[0040] The method that the present invention detects valproic acid in blood comprises the following steps:
[0041] (1) Calibration of standard solution
[0042] (a) Preparation of valproic acid standard stock solution
[0043] Transfer the purchased certified valproic acid standard solution to a 2mL cryopreservation tube to obtain a 1mg / mL valproic acid standard stock solution, and store it at -80°C;
[0044] (b) prepare the standard intermediate solution of valproic acid
[0045] Dilute the above-mentioned valproic acid standard stock solution with a water content of 70% methanol diluent to make seven kinds of standard intermediate solutions of valproic acid containing 2.5mg / L~160mg / L of valproic acid, which contain respectively 2.5, 5, 10, 20, 40, 80, 160 mg / L standard intermediate solution of valproic acid concentration ...
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The invention discloses a method for detecting valproic acid in blood, including the following steps of: preparing a valproic acid standard stock solution, preparing a valproic acid standard intermediate solution, preparing an internal standard working solution, preparing a standard solution, detecting the standard solution by using a liquid chromatograph-mass spectrometer, and fitting to obtain astandard curve equation corresponding to valproic acid, that is, y1 = a * x1 + b; processing to-be-tested blood, detecting the to-be-tested blood by using the liquid chromatograph-mass spectrometer,and calculating the concentration of valproic acid in the to-be-tested blood. According to the method disclosed by the invention, the processing method of a to-be-tested blood sample and the selectionof an internal standard substance make the identification of valproic acid more accurate, the analysis time is short, the interference is small, the internal standard quantification suitability specificity is strong, and the sensitivity is high, and meanwhile, various technical indicators such as the recovery rate, detection limit and precision meet the requirements, and thus the accuracy of testresults is improved, and system errors are eliminated.
Description
technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for detecting valproic acid in blood. Background technique [0002] Valproic acid has a unique chemical structure and pharmacological effects, and is effective for various types of human epilepsy, such as various types of petit mal seizures, myoclonic epilepsy, focal seizures, grand mal seizures, and mixed epilepsy. It is mostly used for various types of epilepsy patients who are ineffective with other antiepileptic drugs, especially for small seizures, so the monitoring of valproic acid has important clinical significance. [0003] Currently, high-performance liquid chromatography is commonly used to test blood samples from patients taking valproic acid. [0004] However, when the valproic acid content in the blood sample is detected by the above method, the blood sample needs to be pretreated, for example, extracted. Since the extraction process usually ...
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Login to View More IPC IPC(8): G01N30/89G01N30/04G01N30/72G01N30/86G01N30/06G01N30/14
CPCG01N30/04G01N30/06G01N30/14G01N30/72G01N30/8634G01N30/89G01N2030/042G01N2030/045
Inventor 雒琴贾永娟倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD