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Cell culture chamber and culture vessel with same

A cell culture and top plate technology, applied in the field of bioengineering, can solve the problems of vibration affecting cell growth and easily destroying cell culture chambers, etc., and achieve the effects of being beneficial to cell growth, improving material exchange, and protecting structural integrity.

Pending Publication Date: 2020-01-07
广州瑞铂茵健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When stirring culture is required, the cell culture chambers collide with each other or the wall of the container in the culture container, which not only easily damages the cell culture chamber, but also produces large vibrations that affect the growth of cells

Method used

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  • Cell culture chamber and culture vessel with same
  • Cell culture chamber and culture vessel with same
  • Cell culture chamber and culture vessel with same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The cell culture chamber is suitable for adherent culture or suspension culture; including but not limited to monolayer static culture in culture dish, dynamic suspension culture in culture bag or agitation culture in culture tank.

[0033] This cell culture chamber fully considers the influence of cell-cell interaction on in vitro cultured cells in the process of large-scale cell culture, and uses membrane technology and co-cultivation methods to make co-cultured cells indirectly have a positive impact on the growth state of applied cells. Of course, the technique is also applicable to the study of various cell interactions (indirect contact).

[0034] Such as figure 1 and figure 2 The cell culture chamber 16 shown includes a top plate 1, a bottom plate 2, a support member, a microporous membrane 3 and an airbag assembly, the top plate 1 and the bottom plate 2 are provided with through holes 4, and the top plate 1 and the bottom plate 2 are opposite to each other up ...

Embodiment 2

[0050] This embodiment is the same as embodiment 1 except the following technical features:

[0051] Such as Figure 4 As shown, the airbag 6 is cylindrical, the number of the airbag 6 is two, and the airbags 6 are arranged at intervals. The two airbags 6 are respectively located on both sides of the top plate 1, and the three sides of each airbag 6 are all convex. Out of the periphery of the microporous membrane located on the outside. This setting can increase the buoyancy of the chamber, making the chamber float above the culture system, which is beneficial to increase the gas exchange of the cells in the chamber, effectively prevent the violent collision between the chamber and the cell culture vessel, protect the structural stability of the chamber, and facilitate cell growth.

Embodiment 3

[0053] This embodiment is the same as embodiment 1 except the following technical features:

[0054] The airbags 6 are cylindrical, and the number of the airbags 6 is three. The airbags 6 are distributed in a ring shape and installed on the top plate 1. One side of each of the airbags 6 protrudes from the microporous membrane positioned on the outside. 3 perimeter. This setting can increase the buoyancy of the chamber, making the chamber float above the culture system, which is beneficial to increase the gas exchange of the cells in the chamber, effectively prevent the violent collision between the chamber and the cell culture vessel, protect the structural stability of the chamber, and facilitate cell growth.

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Abstract

The invention relates to the field of bioengineering, and discloses a cell culture chamber and a culture vessel with the same. The cell culture chamber comprises a top plate, a bottom plate, a supporting piece, microporous membranes and a gasbag assembly; through holes are formed in the top plate and the bottom plate correspondingly, and the top plate and the bottom plate are oppositely arranged in the up-down direction and connected through the supporting piece; the periphery of the top plate and the periphery of the bottom plate are sealed through the corresponding microporous membranes, andthe inner circumference of the top plate and the inner circumference of the bottom plate are sealed through the corresponding microporous membranes, so that sealed storage spaces are defined by the top plate, the bottom plate and the microporous membranes; and a sample adding hole is formed in the top plate and communicates with the storage spaces, and the gasbag assembly is mounted on the top plate and is protruded out of the peripheries of the microporous membranes on the outer side. The cell culture chamber and the culture vessel with the same have the beneficial effects that the cell culture chamber can float on a culture medium, cells in the chamber are fully subjected to gas exchange with the outside, by mounting a gasbag, the chamber can be further prevented from directly and violently colliding with the cell culture vessel, and the structure stability of the chamber is kept.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a cell culture chamber and a culture vessel with the same. Background technique [0002] When carrying out large-scale cell culture in vitro, several cell culture chambers will be placed in the culture vessel, and the cell culture chambers will pile up at the bottom of the culture vessel due to their own weight, which is not conducive to the gas exchange of cells in the cell culture chamber. When stirring culture is required, the cell culture chambers collide with each other or the wall of the container in the culture container, which not only easily damages the cell culture chamber, but also generates large vibrations that affect the growth of cells. Contents of the invention [0003] The object of the present invention is to provide a cell culture chamber with a simple and reasonable structure, which can be floated, in order to overcome the deficiencies in the above prior art. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/22C12M1/00C12M1/02
CPCC12M23/10C12M23/14C12M27/02
Inventor 嵐山芮魏伟李永生
Owner 广州瑞铂茵健康科技有限公司
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