Cell culture chamber and culture vessel with same

Abstract

The invention relates to the field of bioengineering, and discloses a cell culture chamber and a culture vessel with the same. The cell culture chamber comprises a top plate, a bottom plate, a supporting piece, microporous membranes and a gasbag assembly; through holes are formed in the top plate and the bottom plate correspondingly, and the top plate and the bottom plate are oppositely arranged in the up-down direction and connected through the supporting piece; the periphery of the top plate and the periphery of the bottom plate are sealed through the corresponding microporous membranes, andthe inner circumference of the top plate and the inner circumference of the bottom plate are sealed through the corresponding microporous membranes, so that sealed storage spaces are defined by the top plate, the bottom plate and the microporous membranes; and a sample adding hole is formed in the top plate and communicates with the storage spaces, and the gasbag assembly is mounted on the top plate and is protruded out of the peripheries of the microporous membranes on the outer side. The cell culture chamber and the culture vessel with the same have the beneficial effects that the cell culture chamber can float on a culture medium, cells in the chamber are fully subjected to gas exchange with the outside, by mounting a gasbag, the chamber can be further prevented from directly and violently colliding with the cell culture vessel, and the structure stability of the chamber is kept.

Description

technical field

[0001] The invention relates to the field of bioengineering, in particular to a cell culture chamber and a culture vessel with the same. Background technique

[0002] When carrying out large-scale cell culture in vitro, several cell culture chambers will be placed in the culture vessel, and the cell culture chambers will pile up at the bottom of the culture vessel due to their own weight, which is not conducive to the gas exchange of cells in the cell culture chamber. When stirring culture is required, the cell culture chambers collide with each other or the wall of the container in the culture container, which not only easily damages the cell culture chamber, but also generates large vibrations that affect the growth of cells. Contents of the invention

[0003] The object of the present invention is to provide a cell culture chamber with a simple and reasonable structure, which can be floated, in order to overcome the deficiencies in the above prior art. ...

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