Morphology-based sebastiscus marmoratus group identification method

A technology based on morphology and identification method, applied in the field of morphological-based identification of brown scorpionfish population, can solve the problems of time-consuming, complex operation technology, and high requirements for equipment, avoiding death, reducing mortality, and structural protection. Enhanced effect

Active Publication Date: 2020-01-07
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation technology is more complicated, the requirements for instruments and equipment are high, and the whole process is time-consuming.

Method used

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  • Morphology-based sebastiscus marmoratus group identification method
  • Morphology-based sebastiscus marmoratus group identification method
  • Morphology-based sebastiscus marmoratus group identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]The morphological-based identification method of the brown calamari population includes: providing a population sample of the above-mentioned brown calamari; providing a living preservation method for the above-mentioned brown calamari; Obtaining population information; the living preservation method of the above-mentioned scorpionfish sample includes making the fish body contact with a vitreous matrix solution embedded with at least one antioxidant active polysaccharide. This method is convenient for sample preservation and is less affected by the external environment, with small system errors, strong objectivity and identification accuracy, and overcomes the limitation of low discrimination rate of traditional morphological methods. The live preservation method in this method can better stabilize the activity of living samples, prolong the validity period of the live samples, and reduce transportation costs. The interference factors are removed, the error of the detect...

Embodiment 2

[0044] The morphological-based identification method of the brown calamari group comprises the following steps:

[0045] (1) Collected sexually mature individuals of Scorpene scorpionfish in Huizhou, Haikou of China, Hakata Island and Yokosuka offshore of Japan. The sampling numbers were 34, 28, 36 and 30 respectively, with half male and half male, and the body length ranged from 115 to 115. -220mm;

[0046] (2) Place the collected sample live fish in an environment with a temperature of 4-10°C for 1-2 hours, then put the fish body into a vitreous matrix solution with a concentration of 45% for 45 minutes, take it out, and then put the live fish Placed in a normal environment for storage and transportation, the above-mentioned vitreous matrix is ​​a mixture of cyclodextrin and guar gum in equal proportions, and the above-mentioned vitreous matrix solution contains active polysaccharides with a concentration of 3.5%, and the active polysaccharides are spirulina polysaccharides....

Embodiment 3

[0052] The only difference between this example and Example 1 is that 0.04mM abscisic acid and 0.11mM laurocapram are also dispersed in the water body containing the cleaning agent. The cleaning agent destroys the acid-base balance to reduce the viscosity of the mucus, making the mucus To prevent it from being destroyed and falling off, after the abscisic acid and laurocapram penetrate into the mucus, it can destroy the covalent structure between the oligosaccharide peptide and the polypeptide chain contained in the glycoprotein in the mucus, reduce the hydrophobic effect of the mucus, and make the inside of the mucus loose and easier to fall off , the time used is shortened by 10-30%, the mucus is completely removed and the interference factors are reduced, the error of the detection value is reduced, and the identification accuracy is increased. On the other hand, the two can combine with the mucin in the mucus cells of the fish body, And produce a stimulating effect, inhibit...

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Abstract

The invention provides a morphology-based sebastiscus marmoratus population identification method, belongs to the field of germplasm identification, and comprises the following steps: providing a population sample of sebastiscus marmoratus; providing a living body preservation method for the Sebastiscus marmoratus sample; measuring and analyzing morphological indexes of the population samples of the sebastiscus marmoratus to obtain population information; the living body preservation method of the sebastiscus marmoratus sample comprises the step of enabling a fish body to be in contact with avitreous matrix solution embedded with at least one antioxidant active polysaccharide. The identification method provided by the invention is high in objectivity and identification accuracy, small inmeasurement value error, low in measurement difficulty and transportation difficulty, and low in loss and cost; according to the method, the living body valid period of the living body fish can be prolonged, high vitality can still be kept in the environment with the high or low transportation temperature, external mechanical pressure and damage are buffered, the fish body structure protection ishigh, the death rate of the living body fish is low, during measurement, mucus on the body surface of the fish body is rapidly removed, mucus is not continuously released, and system errors are reduced.

Description

technical field [0001] The invention belongs to the field of germplasm identification, and in particular relates to a morphology-based method for identification of scorpionfish populations. Background technique [0002] The brown calamari belongs to the order Scorpioformes, Scorpionidae, and the genus Scorpion, commonly known as stone dog male, tiger head fish, etc. It is a kind of offshore oviparous marine fish. It is mainly distributed in the Northwest Pacific Ocean from Japan to the coastal waters of the Philippines. It is a rocky reef , Benthic fish. Its meat is delicious and has high nutritional value. It is an important offshore economic fish and a potential excellent species for artificial breeding and multiplication and release. At present, the research on the brown calamari mainly focuses on the research of physiology, biochemistry, biology and genetics, and there are few related reports on its group research. The present invention carries out statistical analysis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06K9/62A01K61/10
CPCA01K61/10G06F18/23Y02A40/81
Inventor 徐胜勇蔡珊珊高天翔
Owner ZHEJIANG OCEAN UNIV
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