Carex breviculmis seed radiation mutagenesis breeding method

A technology of green moss grass, mutation breeding, applied in the directions of seed immunity, seed and rhizome treatment, botanical equipment and methods, etc., to achieve the effect of speeding up the breeding process

Active Publication Date: 2020-01-10
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no prior art on the use of 60 Related Reports ...

Method used

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  • Carex breviculmis seed radiation mutagenesis breeding method

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Radiation mutagenic breeding method for the seeds of Carex sativa

[0041] 1) Select the full and consistent green Carex seeds, adopt 60 Co-γ irradiated Carex greens seeds, and the dose of radiation was 75Gy;

[0042] 2) Disinfect the irradiated seeds with 5% NaClO solution for 15 minutes, wash them with distilled water for 3 times, and then soak the seeds with distilled water for 6 hours;

[0043] 3) Spread three layers of filter paper into a petri dish and soak it with distilled water, sow the sterilized seeds into the dish, and germinate under the conditions of a light culture room; wherein, the photoperiod is 14h light / 10h dark, and the light intensity is 2500 Lux, The temperature is 25°C under light conditions and 18°C ​​under dark conditions;

[0044] 4) After 14 days of germination, transfer the robust seedlings to a seedling pot equipped with a seedling-raising substrate, and cultivate them in a solar greenhouse until the 4-leaf stage; wherein, the daytime tem...

Embodiment 2

[0049] The dose of radiation in the radiation-induced mutation breeding method of Carex greens seeds is 100Gy, and the others are exactly the same as in Example 1.

Embodiment 3

[0051] The dose of radiation in the radiation-induced mutation breeding method of Carex sativa seeds is 150Gy, and the others are exactly the same as in Example 1.

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Abstract

The invention provides a carex breviculmis seed radiation mutagenesis breeding method and relates to the technical field of carex mutagenesis breeding. The method includes following steps: 1), adopting 60Co-gamma to radiate carex breviculmis seeds; 2), disinfecting the radiated seeds, and soaking the same; 3), performing germination culture on the soaked seeds to obtain seedlings; 4), transplanting the seedlings into a seedling culture matrix for culture to obtain carex breviculmis in a 4-6-leaf period; 5), transplanting carex breviculmis in the 4-6-leaf period into a field for culture, and screening to obtain floral-leaf carex breviculmis individual plants; 6), performing asexual propagation on the floral-leaf carex breviculmis individual plants to obtain a floral-leaf carex breviculmis strain; 7), performing asexual propagation on the floral-leaf carex breviculmis strain to obtain stable floral-leaf carex breviculmis; performing variety comparison on stable floral-leaf carex breviculmis in the field to obtain carex breviculmis with uniform floral-leaf character. The method is simple and convenient in operation, mature and stable in breeding and short in period.

Description

technical field [0001] The invention relates to the technical field of mutation breeding of carex, in particular to a method for mutation breeding of green carex seeds by radiation. Background technique [0002] Carex leucochlora (Carex leucochlora) is the largest genus of Cyperaceae. It is a perennial herbaceous plant with a wide distribution in my country. Taiwan and other places are distributed. Carex not only has the characteristics of early greening in spring, well-developed rhizomes, long green period, and beautiful shape, but also has the advantages of drought tolerance and low maintenance cost. It can be used as an excellent understory or building shade cover The plant can also be used as an ornamental grass with other plant configuration applications. In the northern region, with the development of low-maintenance and water-saving gardens, the market demand for Carex is increasing year by year. However, there is a shortage of Carex varieties in my country, which c...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H1/04A01C1/08A01C1/00A01G2/00A01G20/00
CPCA01C1/00A01C1/08A01H1/04A01H1/06A01G2/00A01G20/00
Inventor 岳跃森武菊英范希峰滕文军滕珂张辉韩朝温海峰
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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