Potentilla anserine rhizospheric microorganism diversity and flora analysis method based on high-throughput sequencing

An analytical method and microbial technology, applied in the field of soil microbiology, can solve problems such as the influence of the accuracy of rhizosphere microbial community indicators, and achieve the effect of simple detection and low cost

Pending Publication Date: 2020-01-10
QINGHAI UNIV FOR NATITIES
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Problems solved by technology

In addition, different detection techniques also have an impac

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Embodiment

[0043] A preferred embodiment of the present invention provides a high-throughput sequencing-based method for analyzing the diversity and flora of Zerna rhizosphere microorganisms. The specific steps are as follows:

[0044] S1. Collect the soil and rhizosphere soil outside the root zone of the plateau Bracken: first select the soil next to the Bracken plant, remove the ground weeds and litter, remove the 1-2cm topsoil on the soil surface, and collect the soil outside the root zone. Store at -80°C; first select a well-growing mature fern plant, remove ground weeds and litter, remove 1-2 cm of topsoil from the soil surface, and then take the fern plant out of the soil with the root, and remove it by stroke Put down the rhizosphere soil tightly adhered to the roots of Bracken, mix evenly and remove the root hairs, and store at -80°C;

[0045] S2. Extract the microbial total DNA from the soil of the two samples respectively, and then amplify the 16SrDNA in the DNA by PCR;

[004...

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Abstract

The present invention discloses a potentilla anserine rhizospheric microorganism diversity and flora analysis method based on high-throughput sequencing. The potentilla anserine rhizospheric microorganism diversity and flora analysis method based on the high-throughput sequencing comprises the following steps: S1, collecting root zone-exterior soil and rhizospheric soil of plateau potentilla anserine; S2, extracting total microorganism DNA from each sample soil and conducting PCR amplification of 16SrDNA in the total DNA; S3, identifying quality of the amplified products and mixing the PCR amplified products of different samples with equal mass; S4, conducting fragment size and quantitative detection of mixed libraries and high-throughput sequencing; and S5, accurately measuring composition of soil microorganism flora in each sample and conducting bioinformatics analysis. The analysis method can analyze diversity characteristics of the plateau potentilla anserine rhizospheric microorganism flora.

Description

technical field [0001] The invention belongs to the technical field of soil microbiology, and in particular relates to a high-throughput sequencing-based method for analyzing the diversity of microorganisms in the rhizosphere of Bracken and its flora. Background technique [0002] Bracken is rich in starch, which is called "Zenma" or "Ginseng Fruit" in the market. It can cure anemia and malnutrition, and can also be used for sweet food and wine making; Astringent; yellow dye can be extracted from stems and leaves; it is also a nectar source plant and a fodder plant. Bracken is a unique resource plant on the Qinghai-Tibet Plateau. Because it is rich in vitamins, amino acids and various mineral elements, it has always been used by people in Tibetan areas for food, health products and Tibetan medicine. Tibetan medicine calls it "Zhuo Lao Jiu Zeng". [0003] Soil microorganisms are an essential part of soil, and the quality and stability of soil have a great relationship with t...

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/04
CPCC12Q1/6869C12Q2531/113C12Q2535/122C12Q2537/165
Inventor 王雅琼李军乔白世俊韦梅琴
Owner QINGHAI UNIV FOR NATITIES
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