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Plants with increased photorespiration efficiency

A plant and glycolic acid technology, applied in biochemical equipment and methods, using vectors to introduce foreign genetic material, transferase, etc., can solve the problems of no proven effectiveness and no attempt to fully optimize farmers' field photorespiration

Pending Publication Date: 2020-01-10
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SEC OF AGRI (US)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these bypasses, including some modifications, have shown increased plant productivity, their effectiveness under agricultural conditions has not been demonstrated, and no attempt has been made to fully optimize the bypass for photorespiration in farmers' fields

Method used

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  • Plants with increased photorespiration efficiency
  • Plants with increased photorespiration efficiency
  • Plants with increased photorespiration efficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1: Materials and methods

[0104] Plant material and growing conditions

[0105] Arabidopsis Columbia (Col-0) was used as wild type reference. Salk_03569C (plgg1-1), CS859747 (bass6-1) and Salk_052903C (bass6-2) were obtained from the Arabidopsis Biological Resource Center (abrc.osu.edu). Plants at elevated (2000ppm CO 2 ) or ambient (400ppm CO 2 ) in an 8-hour light / 16-hour dark cycle (22°C / 18°C), at 250 μmol·m -2 ·s -1 Growth was performed in a growth chamber (Conviron, USA) using LC1 Sunshine Mix at PAR and 65% relative humidity (RH).

[0106] Chlorophyll fluorescence measurement

[0107] Arabidopsis plants grown in ambient air conditions and moved to low CO 2 Conditions were kept in sealed transparent plastic containers under constant light for 24 hours, similar to (Badger et al., 2009) followed by chlorophyll fluorescence measurements. Chlorophyll fluorescence measurements were performed as previously described (Oxborough and Baker, Plant, Cell & En...

Embodiment 2

[0132] Example 2: Analysis of Arabidopsis bass6 mutant phenotype

[0133] Two independent T-DNA insertion lines targeting the gene At4g22840 lacking expression of the putative chlorophyll inner membrane protein BASS6 were analyzed. To determine whether BASS6 is involved in photorespiration, in ambient CO 2 Two T-DNA lines (bass6-1 and bass6-2) were grown under (400ppm) conditions. The bass6 mutant line displayed a smaller rosette size compared to the wild-type control, similar to the glycolate / glycerate transporter mutant involved in photorespiration plgg1-1 ( figure 2 - with ambient CO 2 Growth ((in a growth chamber at 250 μmol m -2 ·s -1 The light intensity was 400ppm CO for 8 weeks under 8h light / 16h dark cycle (22℃ / 18℃) 2 ) compared to WT, representative photographs of bass6 and plgg1 mutants). At low concentrations of CO 2 Down-illumination of photorespiratory mutants resulted in decreased dark-adapted Fv / Fm chlorophyll fluorescence, likely due to photodamage to p...

Embodiment 3

[0138] Example 3: BASS6 protein localization in the chloroplast envelope

[0139] Although previous studies have shown that BASS6 protein localizes to the chloroplast envelope, a subcellular localization prediction program more strongly supports a mitochondrial localization of BASS6 (Gigolashvili et al., supra). To determine the localization of the BASS6 protein, transient expression of the BASS6-GFP fusion protein was analyzed in both protoplasts and whole leaf tissues of N. benthamiana. Chlorophyll autofluorescence was used to identify chloroplasts ( Figure 7 Panels B, E, H and K). BASS6-GFP signal surrounds chloroplast autofluorescence ( Figure 7 , panels G to I), similar to the known glycolate / glycerate transporter PLGG1 ( Figure 7 , panels D to F), indicating that BASS6 localizes to the chloroplast envelope. In addition, expression of BASS6-GFP or PLGG1-GFP induces the formation of plastid microtubule structures (stromule) ( Figure 7 , star-shaped arrows in panel...

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Abstract

Presented herein are plants with altered photorespiratory characteristics. Disruption of transport proteins involved in shuttling glycolate and / or glycerate results in reductions in photosynthetic rates, reduced plant growth and alterations in gene expression and photosynthetic metabolite profiles. Such disruptions are also combined with introduced genes expressing components of alternate photorespiratory enzyme pathways to increase photosynthetic efficiency.

Description

[0001] cross reference [0002] This application claims priority under 35 U.SC § 119(e) to U.S. Provisional Serial No. 62 / 467,993, filed March 7, 2017, and which is hereby incorporated by reference. technical field [0003] The present disclosure provides plants with altered photorespiratory characteristics. Disruption of transporters involved in shuttling glycolic and / or glyceric acid results in reduced photosynthesis rate, reduced plant growth, and altered gene expression and photosynthetic metabolite profiles. This disruption, when combined with the introduction of genes expressing additional components of the photorespiratory enzyme pathway, increased photosynthesis efficiency. Background technique [0004] Ribulose-1,5-bisphosphate carboxylase / oxygenase (RubisCO) catalyzes the conversion of ribulose-1,5-bisphosphate (RuBP) to CO 2 immobilization, yielding two molecules of 3-phosphoglycerate (3-PGA). However, at 25°C and current CO 2 At low levels, about 25% of the r...

Claims

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Application Information

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IPC IPC(8): C12N15/82
CPCC12N15/8262C12N15/8269C12N15/8218C12N15/8245C12Y101/99014C12Y203/03009
Inventor 唐纳德·R·奥尔特保罗·F·索思伯克利·沃克
Owner THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SEC OF AGRI (US)
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