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Lactobacillus buchneri 9-2 for producing 1,2-propanediol, and application of lactobacillus buchneri 9-2 in silage

A technology of Lactobacillus Brucella and silage, which is applied to Lactobacillus Brucella 9-2 and its application field in silage, can solve difficult problems, improve safety, prevent spoilage, and improve aerobic The effect of stability

Active Publication Date: 2020-01-17
甘肃普诺贝康生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this field, the method of anaerobic fermentation is generally used to control the spoilage of silage, but it is difficult to completely anaerobic from the beginning of silage, and it can only ensure the control of spoilage during storage, and cannot avoid the spoilage in the subsequent use process. spoilage

Method used

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  • Lactobacillus buchneri 9-2 for producing 1,2-propanediol, and application of lactobacillus buchneri 9-2 in silage
  • Lactobacillus buchneri 9-2 for producing 1,2-propanediol, and application of lactobacillus buchneri 9-2 in silage

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preparation example Construction

[0020] The invention provides a method for preparing silage, which comprises the following steps: mixing the 1,2-propanediol-producing Lactobacillus Brucella 9-2 with the whole corn plant, vacuum packaging, and silage.

[0021] In the present invention, the described 1,2-propanediol-producing Lactobacillus brunici 9-2 is mixed with the whole plant corn; in the present invention, the 1,2-propanediol production is based on the mass of the whole plant corn The amount of Lactobacillus Brucella 9-2 is preferably (0.5~5)×10 6 cfu / g, more preferably (0.8~1.2) ×10 6 cfu / g, most preferably 1.0×10 6 cfu / g. In the present invention, there is no special limitation on the cultivation method of the 1,2-propanediol-producing Lactobacillus brauchneri 9-2, and the conventional cultivation method of Lactobacillus brauchneri in the field can be adopted. In the present invention, the whole-plant corn is conventional whole-plant corn in the art, and the whole-plant corn is preferably mixed with...

Embodiment 1

[0025] Add 1×10 Lactobacillus Brucella (9-2) and Lactobacillus Brucella LB1 (Lactobacillus Brucella 40788) that does not produce 1,2-propanediol, respectively. 6 cfu / g in whole corn. Add the same volume of distilled water to the blank control group. Each treatment was replicated three times, and 300 g of each replicate was packed in a polyethylene vacuum bag and vacuumized, and ensiled at 25°C for 90 days. To detect its effect on the fermentation quality and aerobic stability of silage.

[0026] Table 1 The number of microorganisms and pH of corn silage after 90 days

[0027]

[0028] It can be seen from Table 1 that the number of lactic acid bacteria in the silage corn added with 9-2 was higher than that of the control and LB1 added silage corn, and the number of mold such as yeast and mold was significantly lower than that of the control and LB1 added group. The pH of silage corn added with 9-2 was lower than that of the other two groups, so the addition of 9-2 silage ...

Embodiment 2

[0033] Add the described Lactobacillus Brucella (9-2) to 5×10 6 cfu / g in the whole corn plant, each 300g is packed in a polyethylene vacuum bag and vacuumized, and ensiled at 25°C for 90 days.

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Abstract

The invention provides a lactobacillus buchneri 9-2 for producing 1,2-propanediol, and application of the lactobacillus buchneri 9-2 in silage, and belongs to the technical field of microorganism application. The lactobacillus buchneri 9-2 for producing the 1,2-propanediol is collected in the China General Microbiological Culture Collection Center with a collection number of CGMCC No.18376. The lactobacillus buchneri 9-2 for producing the 1,2-propanediol is taken as a putrefying bacteria inhibitor and can be applied to the preparation of the silage. The lactobacillus buchneri 9-2 for producingthe 1,2-propanediol and a whole-crop corn are mixed and packaged in vacuum, and an obtained product is fermented for a period of time to obtain the silage. By use of the method, the growth of silageputrefying bacteria can be effectively inhibited, the aerobic stability of the silage is improved, the silage is prevented from being rotten and going bad, and therefore, the safety of the silage in ataking process is improved.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a 1,2-propanediol-producing Lactobacillus Brucella 9-2 and its application in silage. Background technique [0002] In the natural environment, feed silage is prone to corruption and deterioration during the process of taking it. The toxins produced during the deterioration process have a greater negative impact on the safety and production performance of livestock, and the animal products produced may even affect human health. On the other hand, the mildew and deterioration of silage will cause serious waste of silage and increase the breeding cost of animal husbandry. [0003] How to suppress the silage spoilage becomes the key to the application of silage. In this field, the method of anaerobic fermentation is generally used to control the spoilage of silage, but it is difficult to completely anaerobic from the beginning of silage, and it can onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/225
CPCA23K30/18C12R2001/225C12N1/205A23V2400/11
Inventor 郭旭生黄志鹏许冬梅柯文灿
Owner 甘肃普诺贝康生物科技有限责任公司