Agrobacterium-mediated genetic transformation method using mature embryo ofsorghum as explant to induce callus

Abstract

The invention belongs to the technical field of plant genetic engineering, and provides an agrobacterium-mediated genetic transformation method using the mature embryo of sorghum as an explant to induce callus for the disadvantageous that in traditional sorghum genetic transformation systems, material drawing by using young embryos and young ears as explants is seasonally restricted and also for the tedious operation that embryo stripping is needed for other crops in which the mature crops are used as the explants. Sorghum seeds which are germinated and then appear white are utilized as objects for agrobacterium infection and the explants for callusogenesis induction, resistant callus are obtained through screening and cultivation, and then a regenerated and transformed plant is obtained through subculture, differentiation, rooting, and transplantation. The disadvantages that in an existing sorghum genetic transformation system, the material drawing by using the young embryos and youngears as the explants is seasonally restricted is avoided, the tedious operation that the embryo stripping is needed for other crops in which the mature crops are used as the explants is overcome, andthe sorghum genetic transformation efficiency is greatly improved. According to the agrobacterium-mediated genetic transformation method using the mature embryo of sorghum as the explant to induce the callus, references and technical approaches are provided for performing genetic improvement of sorghum by using biotechnology.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to an agrobacterium-mediated genetic transformation method for inducing callus with mature sorghum embryos as explants. Background technique [0002] Sorghum (Sorghum bicolor (L.) Moench) is an important food, feed and wine-making raw material in the world. Because of its drought and flood resistance, salt-alkali tolerance, and barrenness tolerance, it has been used as a pioneer crop in dryland farmland. At present, due to the lack of sorghum germplasm resources and the incompatibility of sexual hybridization, the progress of variety improvement using traditional breeding methods is slow. Modern genetic engineering technology provides a new way for rapid genetic improvement of sorghum. Although sorghum, like other crops, has been researched on the genetic transformation system for a long time, the progress has been slow, and it is recognized as one of t...

AI Technical Summary

Problems solved by technology

The pollen tube passage method for transgenic sorghum, although it does not undergo tissue culture, has disadvantages such as difficult operation, low conversion rate, and poor repeatability. Currently, it is less used than the previous four methods

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