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Agrobacterium-mediated genetic transformation method using mature embryo ofsorghum as explant to induce callus

A genetic transformation method, Agrobacterium-mediated technology, applied in the field of genetic transformation of sorghum mature embryos as explants to induce callus, can solve the problems of difficult operation, low transformation rate, poor repeatability, etc., and achieve operational Simple, efficient, low cost effect

Pending Publication Date: 2020-01-17
AGRI BIOTECH RES CENT OF SHANXI PROVINCE +1
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Problems solved by technology

The pollen tube passage method for transgenic sorghum, although it does not undergo tissue culture, has disadvantages such as difficult operation, low conversion rate, and poor repeatability. Currently, it is less used than the previous four methods

Method used

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  • Agrobacterium-mediated genetic transformation method using mature embryo ofsorghum as explant to induce callus
  • Agrobacterium-mediated genetic transformation method using mature embryo ofsorghum as explant to induce callus
  • Agrobacterium-mediated genetic transformation method using mature embryo ofsorghum as explant to induce callus

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Embodiment Construction

[0031] The present invention is further explained below in conjunction with specific examples. The embodiment is to further illustrate the present invention, rather than limit it, and the purpose is to allow technical researchers to understand the present invention more intuitively. The present invention is not limited in any way, and appropriate modifications can be made without departing from the essence and scope of the present invention.

[0032] An Agrobacterium-mediated genetic transformation method using sorghum mature embryos as explants, using germinated sorghum seeds as the objects of Agrobacterium infection, and using them as explants to induce callus generation, after screening and cultivation, The resistant callus is obtained, and then regenerated and transformed plants are obtained through subculture, differentiation, rooting, and transplanting.

[0033] 1. Experimental method and experimental process The specific steps are as follows:

[0034] 1. Transformatio...

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Abstract

The invention belongs to the technical field of plant genetic engineering, and provides an agrobacterium-mediated genetic transformation method using the mature embryo of sorghum as an explant to induce callus for the disadvantageous that in traditional sorghum genetic transformation systems, material drawing by using young embryos and young ears as explants is seasonally restricted and also for the tedious operation that embryo stripping is needed for other crops in which the mature crops are used as the explants. Sorghum seeds which are germinated and then appear white are utilized as objects for agrobacterium infection and the explants for callusogenesis induction, resistant callus are obtained through screening and cultivation, and then a regenerated and transformed plant is obtained through subculture, differentiation, rooting, and transplantation. The disadvantages that in an existing sorghum genetic transformation system, the material drawing by using the young embryos and youngears as the explants is seasonally restricted is avoided, the tedious operation that the embryo stripping is needed for other crops in which the mature crops are used as the explants is overcome, andthe sorghum genetic transformation efficiency is greatly improved. According to the agrobacterium-mediated genetic transformation method using the mature embryo of sorghum as the explant to induce the callus, references and technical approaches are provided for performing genetic improvement of sorghum by using biotechnology.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to an agrobacterium-mediated genetic transformation method for inducing callus with mature sorghum embryos as explants. Background technique [0002] Sorghum (Sorghum bicolor (L.) Moench) is an important food, feed and wine-making raw material in the world. Because of its drought and flood resistance, salt-alkali tolerance, and barrenness tolerance, it has been used as a pioneer crop in dryland farmland. At present, due to the lack of sorghum germplasm resources and the incompatibility of sexual hybridization, the progress of variety improvement using traditional breeding methods is slow. Modern genetic engineering technology provides a new way for rapid genetic improvement of sorghum. Although sorghum, like other crops, has been researched on the genetic transformation system for a long time, the progress has been slow, and it is recognized as one of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84C12N15/54A01H5/00A01H6/46A01H4/00
CPCC12N15/8205A01H4/001A01H4/008C12N9/1092C12Y205/01019
Inventor 郝曜山王瑞王亦学王晓清张欢欢孙毅周福平
Owner AGRI BIOTECH RES CENT OF SHANXI PROVINCE
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