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Method for passively monitoring antibiotic content in aquaculture water body in situ

A technology for aquaculture water and antibiotics, which is applied in the preparation and sampling of measuring devices, samples for testing, etc., can solve the problems of large adsorption capacity, large specific surface area, and short time required to achieve large adsorption capacity, large specific surface area, Make simple effects

Pending Publication Date: 2020-01-17
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the XDA-1 resin material has a stable structure, high porosity, large specific surface area, and large adsorption capacity. It has a good adsorption effect on antibiotics in aquaculture water, takes a short time, and has accurate monitoring results, making up for the inadequacy of instantaneous active sampling method concentration monitoring. Insufficient in continuity and large errors, it is suitable for the detection of low-concentration antibiotics in aquaculture water with an actual ambient temperature of 15-25°C, and solves the problems of time-consuming, complicated process and high cost in traditional monitoring methods of antibiotic concentration in aquaculture water

Method used

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  • Method for passively monitoring antibiotic content in aquaculture water body in situ
  • Method for passively monitoring antibiotic content in aquaculture water body in situ
  • Method for passively monitoring antibiotic content in aquaculture water body in situ

Examples

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Embodiment

[0042] Prepare 1 L of antibiotic solution with a concentration of 0.3 ng / mL, and adjust the pH value between 7.0 and 8.5 with hydrochloric acid or sodium hydroxide (1 mol / L). 11 DGTs were simultaneously deployed in 11 beakers filled with 1L antibiotic aqueous solution with a concentration of 0.3ng / mL, each beaker was wrapped with tinfoil (to prevent the photodegradation of antibiotics), and placed at a speed of 120-140r / mL at the same time. Take out a DGT device at 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, 4.5h, 5h, 5.5h, and 6h in a constant temperature shaker at 25°C for min. Align the opening of the DGT with an awl and gently pry it open to take out the adsorption film. Then place the adsorption membrane in an elution test tube containing 10 mL of methanol solution, remove the adsorption membrane after ultrasonication for 15 to 20 min, and concentrate the eluent to 50 ul (V e ), to determine the concentration of antibiotics in the eluate (C e ), and then calculate the diffusion c...

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Abstract

The invention discloses a method for passively monitoring antibiotic content in an aquaculture water body in situ. XDA-1 resin is used as an adsorption membrane material and agarose gel is used as a diffusion membrane material. An obtained adsorption membrane, a diffusion membrane, and a round polyether sulfone filtering membrane are assembled as a DGT apparatus, and the apparatus is used to adsorb antibiotics in the aquaculture water body and monitor a concentration of the antibiotics. A XDA-1 resin material has a stable structure, high porosity, a large specific surface area, and a large adsorption capacity, has a good adsorption effect on the antibiotics in the aquaculture water body, requires a short time, has an accurate monitoring result, compensates for shortcomings of discontinuityand a relatively large error in concentration monitoring of an instantaneous active sampling method, is applicable to monitoring of low-concentration antibiotics in an aquaculture water body with anactual ambient temperature of 15 DEG C to 25 DEG C on the spot, may be widely promoted, and solves problems such as a long consumed time, a complex process, and high costs in a conventional method formonitoring an antibiotic concentration in the aquaculture water body.

Description

technical field [0001] The invention belongs to the technical field of aquaculture water monitoring, and in particular relates to a method for in-situ passive monitoring of antibiotic content in aquaculture water. Background technique [0002] When antibiotics are used in aquaculture water, only a small part can be absorbed and utilized by aquatic organisms, and a large amount of remaining antibiotics remain in the aquaculture environment in the form of prototypes or metabolites or enter the natural water environment with the discharge of aquaculture wastewater. Antibiotic residues can induce the resistance of microorganisms and bacteria, and pose a threat to aquatic animals, plants and even human health. At present, most of the traditional antibiotic monitoring methods are to actively collect instantaneous water samples and measure them on the computer after solid-phase extraction. This method takes active sampling, and the sample concentration is instantaneous, and the mea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/00G01N1/40
CPCG01N1/405G01N30/00
Inventor 李娟英文聚陈以芹郑儒懿盛博维
Owner SHANGHAI OCEAN UNIV
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