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Research on hplc fingerprint of a kind of Miao medicine Heiguteng and its multi-component content determination method

A technology of fingerprints and black bone vine, which is applied in the direction of measuring devices, material separation, and analysis of materials, and can solve problems such as differences in the content of black bone vine components

Active Publication Date: 2022-03-11
贵州中医药大学
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] However, there is a big difference in the content of each component of Teng Teng from different origins. In order to provide a basis for the quality evaluation of Teng Teng medicinal material, a method for HPLC fingerprinting and determination of multi-component content of Miao medicine Teng Teng was developed.

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  • Research on hplc fingerprint of a kind of Miao medicine Heiguteng and its multi-component content determination method
  • Research on hplc fingerprint of a kind of Miao medicine Heiguteng and its multi-component content determination method
  • Research on hplc fingerprint of a kind of Miao medicine Heiguteng and its multi-component content determination method

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Embodiment Construction

[0093] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0094] Example. A kind of Miao medicine Heiguteng HPLC fingerprint chromatogram research and multi-component content determination method, the composition is as follows Figure 1-3 shown, including the following steps:

[0095] a. Preparation of the test solution: Pulverize the syringa vine through a 24-mesh sieve, store the powder in a desiccator, accurately weigh 1.000g of the syringa powder, put it in a 50mL conical flask with a stopper, add 20mL of 50% methanol , weigh the weight, use power 200W, frequency 40KHz to carry out ultrasonic extraction for 30min, after cooling to room temperature, supplement the weight lost in the ultrasonic process with 50% methanol, shake well, pass through a 0.22μm microporous membrane, and get the subsequent filtrate, namely have to;

[0096] b. Preparation of ...

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Abstract

The invention discloses a method for researching the HPLC fingerprint spectrum of the Miao medicine vine vine and measuring the content of multiple components. Using HPLC method to establish the fingerprints of Teng Teng from different origins, and determine the content of four caffeoylquinic acids in it to provide a basis for the quality evaluation of Teng Teng; the chromatographic column is Xtimate C 18 (4.6mmx250mm, 5μm); use 0.1% phosphoric acid aqueous solution (A)-acetonitrile (B) as mobile phase, gradient elution; flow rate 1mL / min; column temperature 30℃; injection volume 10μL, detection wavelength 330nm; use traditional Chinese medicine The Chromatographic Fingerprint Similarity Evaluation System (2012 Edition) performs similarity analysis on the samples, and through the HPLC fingerprint and content determination of the vine, can simultaneously determine the content of 4 kinds of caffeoylquinic acid chemical components. There are large differences in the components of each species, and the content of each component has a certain relationship with the place of origin, which lays the foundation for the later research on the quality standards of the Miao medicine Heiguteng.

Description

technical field [0001] The invention relates to a method for fingerprint spectrum research and multi-component content determination, in particular to a method for HPLC fingerprint spectrum research and multi-component content determination of the seedling medicine Aegypti japonica. Background technique [0002] Black bone vine is the dry root or whole plant of Periploca Linn. (Periploca forrestii Schltr.) in the family Asclepiadaceae. Properties: pungent, bitter, hot in nature; slightly poisonous. Enter the Leng Jing. Expelling wind and removing dampness, promoting blood circulation and eliminating carbuncle. Cure mainly bruises, rheumatic arthralgia, amenorrhea, mastitis, fractures, etc. It is a folk medicine widely used in the treatment of closed soft tissue injuries, rheumatism and rheumatoid diseases. The chemical components include: cardiac glycosides, flavonoids, caffeoylquinic acids, phenylpropanoids, triterpenoids, steroids, etc. [0003] However, there are lar...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8686
Inventor 刘刚刘育辰唐萍代玉洁
Owner 贵州中医药大学