A method for optimizing the preservation of pathological gross specimens

A specimen and pathological technology, applied in the field of specimen preservation, can solve the problems that affect the observation and shooting of specimens, the difficulty of bubbles overflowing, and the infiltration of tissues, etc., and achieve the effect of avoiding inability to overflow, optimizing preservation, and improving fluidity and permeability.

Active Publication Date: 2021-11-19
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, glycerin with too high a concentration (40%) is too viscous, difficult to penetrate into tissues, and makes it difficult for bubbles in the preservation solution to overflow, which affects the observation and shooting of specimens

Method used

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  • A method for optimizing the preservation of pathological gross specimens
  • A method for optimizing the preservation of pathological gross specimens
  • A method for optimizing the preservation of pathological gross specimens

Examples

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Comparison scheme
Effect test

Embodiment 1

[0049] Ulcerative intestinal tuberculosis specimen

[0050] The original specimen of ulcerative intestinal tuberculosis was soaked in 10% formalin fixative solution for a long time, and the color of the specimen was dull, such as Figure 4 As shown, after being processed and preserved by the optimized preservation method of the present invention, the specimen returns to the color close to the fresh specimen, as Figure 5 shown.

Embodiment 2

[0052] Cardiac hypertrophy specimen

[0053] The original specimen of cardiac hypertrophy has been soaked in 10% formalin fixative for a long time, and the color of the specimen is dull, such as Figure 6 Shown, after being processed by the present invention, specimen returns to the color and luster close to fresh specimen, as Figure 7 shown.

Embodiment 3

[0055] Transitional cell carcinoma of the renal pelvis

[0056] The original specimen of transitional cell carcinoma of the renal pelvis was soaked in 10% formalin fixative for a long time, and the color of the specimen was dull, such as Figure 8 Shown, after being processed by the present invention, specimen returns to the color and luster close to fresh specimen. After using 40% sodium glycerol acetate preservation solution, a large number of bubbles appeared in the container, and the bubbles did not disappear after a week, such as Figure 9 Shown; After using 20% ​​sodium glycerol acetate used in the present invention instead, the bubbles in the container disappear almost completely, as Figure 10 As shown, the 20% sodium glycerol acetate preservation solution has good fluidity and permeability, and avoids the situation that a large number of bubbles cannot overflow.

[0057]After converting the specimen picture into an 8-channel grayscale picture, use the image analysis...

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Abstract

The present invention provides a method for optimizing the preservation of pathological gross specimens, which includes the following steps: (1) taking out the specimens stored in traditional fixative solution, soaking them in 10% neutral formalin for one week; (2) taking out the specimens, Rinse with tap water overnight to remove formaldehyde in the tissue; (3) Place the specimen in a container filled with 80% ethanol for 6‑8 hours; (4) Take the specimen out of the container in step (3) , soaked in 95% ethanol for two hours; (5) soaked in 20% sodium glycerol acetate solution for one week to dissolve the ethanol in the specimen and separate the precipitate in the specimen; (6) Take out the specimen, Place the specimens in an acrylic container filled with freshly prepared 20% sodium glycerol acetate preservation solution for long-term preservation. The invention not only improves the refractive index of the liquid, meets the needs of digital photography, but also ensures that the preservation liquid has better fluidity and permeability, and avoids the situation that a large number of bubbles cannot overflow.

Description

technical field [0001] The invention belongs to the technical field of specimen preservation, and relates to a method for optimizing the preservation of gross pathological specimens, in particular to a method for optimizing the preservation of gross pathological specimens with low lipid content. Background technique [0002] Ordinary pathological gross specimen preservation solution generally uses 10% formalin (4% formaldehyde solution). After a long time of preservation, the color of the specimen will fade, the color of the tissue with high blood content will become dark, and the formaldehyde gas is volatile, causing environmental damage. Pollution, harmful to human body and other disadvantages. [0003] Existing environment-friendly fixatives generally use sodium acetate or potassium acetate as preservatives, adding different concentrations of glycerin to increase the refractive index of the liquid, which is conducive to storing specimens in acrylic containers for digital ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 陆鹏季菊玲
Owner NANTONG UNIVERSITY
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