L-lactate detection reagent kit

Abstract

The invention relates to an L-lactate detection reagent kit. The reagent kit disclosed by the application of the invention comprises a 20mmol / L-300mmol / L buffer solution of which pH is 6-9, 0.1-2.0ku / L lactic acid oxidase, 0.1ku / L-10ku / L peroxidase, 0.1-30mmol / L 4-aminoantipyrine, 0.1mmol / L-30mmol / L chromogen, 0.01%-5% of a preservative, a nonionic surface activating agent, an ampholytic surfactant and a protein type stabilizing agent. The reagent kit disclosed by the application has the advantages that the reagent kit can resist Vc interference, the reagent kit does not need calibration afterbeing stored in an uncapped manner for 1 month, the appearance changes of a reagent within the validity period are inapparent, and the reagent kit is not a non-dry-powder liquid reagent.

Description

technical field [0001] This application relates to the field of clinical testing. In particular, the present application relates to a reagent for determining the content of L-lactate. Background technique [0002] Lactic acid is an intermediate product of sugar metabolism (glycolysis), which is catalyzed by lactate dehydrogenase to generate pyruvate. Lactic acid is mainly derived from skeletal muscle, brain, skin, renal medulla and red blood cells. [0003] The lactate cycle is the conversion of glucose to lactic acid in the peripheral tissues, which in turn is converted to glucose in the liver. Extrahepatic lactate is cleared by oxidation of skeletal muscle and renal cortex. The increase of lactic acid production will promote the removal of lactic acid by the liver, but when the concentration of lactic acid exceeds 2 mmol / L, the uptake of lactic acid by the liver will be saturated, and lactic acidosis will occur. [0004] In muscle cells, elevated lactate concentrations...

AI Technical Summary

Problems solved by technology

[0009] The currently commercially available L-lactate kit is in dry powder state, which is not convenient for hospitals to use, and the stability of the reagent after reconstitution is poor, so it cannot be placed for a long time

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