Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing replantation material for autologous skull

A technology of autologous bone and skull, which is applied in the field of preparation of autologous skull for replantation materials, which can solve the problems of inability to repair defects, easy pollution, and decline in mechanical properties, achieve good bone transduction and osteoinductivity, and promote bone growth , the effect of accelerating bone healing

Inactive Publication Date: 2020-02-04
成都奇璞生物科技有限公司
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of in vivo preservation is that it requires patients to bear additional risks of surgical trauma and infection, as well as the phenomenon of autogenous bone resorption during in vivo preservation, which makes it impossible to repair the original defect
It is easy to be polluted during cryopreservation, and the mechanical properties are significantly reduced after high-temperature disinfection. Only the chemical reagents have poor biocompatibility. They all experience bone resorption during the skull replantation process, making it impossible to repair the original defect.
At present, it has always been a difficult problem to solve the problems of good biocompatibility, no postoperative inflammatory reaction and autoabsorption that can meet the clinical application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing replantation material for autologous skull

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, the present invention is made by following steps in conjunction with the accompanying drawings and specific implementation methods:

[0037] (1) Decellularization and skull drilling: such as figure 1 As shown, the fresh skull bone flap was taken out in a clean environment, put into a sterile sealed box and filled with ultrapure water, placed in the refrigerator at -80°C for 10 hours, and quickly rewarmed in a 37°C water bath for about 0.5 hours; During the process of cooling and rewarming, the tissue cells are fully ruptured and the cells are autolyzed. Use surgical tools to remove the soft tissue on the surface of the skull; use a drill to drill holes in the skull with a diameter of about 0.5 cm and a hole spacing of 2 cm. The edge of the bone is about 3 cm away from the drill hole without drilling. Place the autologous bone material again as above for freezing at 80°C and rapid rewarming in a 37°C water bath, repeat freezing and thawing twice, and use a...

Embodiment 2

[0054] Embodiment two, in conjunction with accompanying drawing and specific implementation mode, the present invention adopts following steps to make:

[0055] (1) Decellularization and skull drilling: Fresh skull flaps were taken out in a clean environment and placed in a sterile sealed box and injected with ultrapure water, placed in a refrigerator at -80°C for 18 hours, and then quickly removed in a 37°C water bath. Rewarming is about 0.5 hours; the tissue cells are fully ruptured during the cooling and rewarming process, and the cells are autolyzed. Use surgical tools to remove the soft tissue on the surface of the skull; use a drill to drill holes in the skull with a diameter of about 0.7 cm and a hole spacing of 2.5 cm. The edge of the bone is about 2.5 cm away from the drill hole without drilling. Place the autologous bone material again as above for freezing at 80°C and rapid rewarming in a 37°C water bath, repeat freezing and thawing twice, and use a mixture of 0.05%...

Embodiment 3

[0063] Embodiment three, in conjunction with accompanying drawing and specific implementation mode, the present invention adopts following steps to make:

[0064] (1) Decellularization and skull drilling: Fresh skull flaps were taken out in a clean environment and placed in a sterile sealed box and filled with ultrapure water. They were placed in a refrigerator at -80°C for 14 hours, and quickly cooled in a 37°C water bath. Rewarming is about 0.5 hours; the tissue cells are fully ruptured during the cooling and rewarming process, and the cells are autolyzed. Use surgical tools to remove the soft tissue on the surface of the skull; use a drill to drill holes in the skull with a diameter of about 0.5 cm and a hole spacing of 2 cm. The edge of the bone is about 3 cm away from the drill hole without drilling. Place the autologous bone material again as above for freezing at 80°C and rapid rewarming in a 37°C water bath, repeat freezing and thawing twice, and use a mixture of 0.05%...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing a replantation material for an autologous skull. The method includes the steps that an autologous skull sample is extracted and pretreated; the pretreated autologous skull sample is repeatedly freeze-thawed and treated by a protease solution to remove cells; the autologous skull sample after cell removal is decalcified in an acid solution; the decalcified autologous skull sample is degreased and inactivated; the inactivated autogenous skull sample is freeze-dried and sterilized; and the sterilized autogenous skull sample is added with growth factors to obtain the replantation material. The replantation material prepared by the provided method has good biocompatibility after being used, and no inflammatory reaction and autologous absorption after operation.

Description

technical field [0001] The invention relates to the field of biomaterials, in particular to a method for preparing an autologous skull for replantation materials. Background technique [0002] Decompressive craniectomy is an effective treatment for patients with increased intracranial pressure. In order to achieve the purpose of intracranial decompression, the removed bone flap cannot be replanted immediately, so the patient needs to wait until the patient's condition is stable before undergoing skull defect prosthetics. At present, materials for skull defect repair can be divided into metal materials, polymer materials, allogeneic bone, heterogeneous allogeneic bone and other materials. And temperature insulation and other aspects completely replace the autogenous skull. [0003] Autologous skull has the advantages of good tissue biocompatibility and mechanical properties, good osteoinduction and bone conduction, and the skull flap fully meets the anatomical and physiolog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/54
CPCA61L27/3608A61L27/3687A61L27/3691A61L27/54A61L27/365A61L2430/02
Inventor 岳崇霞宋磊
Owner 成都奇璞生物科技有限公司