Malachite green detection method and detection device and probe manufacturing method

A malachite green and detection method technology, applied in the direction of measuring devices, test sample preparation, instruments, etc., can solve the problems of time extension, rough coating thickness, difficult elution, etc., to reduce interference, simplify operation steps, The effect of controllable coating thickness

Pending Publication Date: 2020-02-04
深圳至秦仪器有限公司 +1
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  • Abstract
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  • Claims
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Problems solved by technology

The thickness of the coating prepared by this method is relatively rough, and the template molecules or adsorbed targets in the inner coating are difficult to elute, and the time required to reach the equilibrium state of extraction or desorption is prolonged

Method used

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  • Malachite green detection method and detection device and probe manufacturing method
  • Malachite green detection method and detection device and probe manufacturing method
  • Malachite green detection method and detection device and probe manufacturing method

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Embodiment Construction

[0050] Embodiments of the present invention will be described in detail below. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.

[0051] It should be noted that when an element is referred to as being “fixed” or “disposed on” another element, it may be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element. In addition, connection may be for either a securing function or a circuit / signal communication function.

[0052] It is to be understood that the terms "length", "width", "top", "bottom", "front", "rear", "left", "right", "vertical", "horizontal", "top" , "bottom", "inner", "outer" and other indicated orientations or positional relationships are based on the orientations or positional relationship...

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Abstract

The invention discloses a malachite green detection method and detection device and a probe manufacturing method. The detection method includes the following steps: S1, preparing polymer material imprinted with malachite green template molecules; S2, grinding the polymer material into polymer powder; S3, eluting the template molecules on the polymer powder; S4. gluing the polymer powder, of whichthe template molecules are eluted, to a surface of an extraction substrate to form a solid-phase microextraction probe; S5, applying a to-be-detected sample to the solid-phase microextraction probe; and S6, using the solid-phase microextraction probe and a mass spectrometer in combination to carry out detection about malachite green on the to-be-detected sample. The detection method can effectively improve template molecule elution efficiency in a probe manufacturing process, reduces interference of the template molecules with the to-be-detected target substance, effectively improves binding efficiency of the probe and an eluent during detection, and improves elution efficiency and detection efficiency of the to-be-detected target substance during mass spectrometry detection.

Description

technical field [0001] The invention relates to a material detection technology, in particular to a malachite green detection method, a detection device and a method for making a detection probe. Background technique [0002] Malachite green is a triphenylmethane industrial dye with green metallic luster, and its chemical formula is C 23 h 25 C1N 2 , is widely used in aquaculture because of its low cost and good effect on preventing saprolegniasis, gill mold and melon worm disease. However, a number of studies have proved that malachite green is a potential carcinogenic, teratogenic and mutagenic toxin to fish and humans, so it has been listed as a prohibited drug by the Food and Drug Administration of Canada, the European Union and the United States. Therefore, it is necessary to develop a rapid sample pretreatment process for aquatic products for the supervision of food safety. [0003] Traditional sample processing is usually complex and cumbersome, including multi-st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34G01N27/62
CPCG01N1/28G01N1/34G01N27/62
Inventor 张兵郭成安钟仕花瞿翠兰
Owner 深圳至秦仪器有限公司
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