Skin care product using iPS cell culture fluid as main ingredient and preparation method thereof

A technology of cell culture and skin care products, which is applied in the field of skin care products and its preparation, can solve the problems of reasonable formula and fewer processes, and achieve the effects of overcoming odor, enhancing activity, and safe and toxic side effects

Inactive Publication Date: 2020-02-07
盛世荣恩生物科技有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are few reasonable formulas and processes that can efficiently produce stem cell liquid and successfully make stem cell liquid into skin care products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Skin care product using iPS cell culture fluid as main ingredient and preparation method thereof
  • Skin care product using iPS cell culture fluid as main ingredient and preparation method thereof
  • Skin care product using iPS cell culture fluid as main ingredient and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] A method for preparing a skin care product as described above with iPS (induced pluripotent stem cells, iPS cells) cell culture fluid as the main component, comprising the following steps:

[0031] (1) Preparation of iPS cell liquid: collect peripheral blood cells from human blood samples, and artificially induce them with an iPS stem cell induction kit. After two to three weeks of induction, obtain iPS cell lines and carry out cell culture. Collect the cell supernatant, concentrate the collected supernatant, concentrate according to the ratio of 1:1-1:20, and keep it for subsequent use;

[0032] (2) Preparation of base liquid: add components A and B to a high-speed mixer and heat to 80-85°C for high-speed mixing and stirring. When the high-speed mixer cools below 40°C, add component C to high-speed mixing and stirring, and then put in Mix and stir component D at a high speed, and put in component E after the high-speed mixer cools down to room temperature;

[0033] (3...

Embodiment 1

[0036] The invention provides a skin care product mainly composed of iPS cell culture fluid, which is composed of 4% iPS cell fluid and 96% base fluid in terms of mass percentage.

[0037] The iPS cell solution preparation steps are as follows:

[0038] That is, peripheral blood mononuclear cells (PBMC) are collected from human blood samples, infected with CytoTune-iPS2.0LG (cell reprogramming kit manufacturer: Japan ID Pharma Co.Ltd), and cultured under conditions of no supply and no serum After 21 days, human iPS cell colonies were formed, and then human iPS cell lines were established based on the human iPS cell colonies.

[0039] The specific preparation process is as follows: the peripheral blood sample is diluted with 1.5 times of PBS buffer (phosphate buffered saline solution, manufacturer: Thermo Fisher Scientific) and placed in lymphocyte separation medium (brand: Lymphoprep, manufacturer: Alere Technologies AS); then , centrifuge at 400g at room temperature for 30 m...

Embodiment 2

[0049] The invention provides a skin care product mainly composed of iPS cell culture fluid, which is composed of 2% iPS cell fluid and 98% base fluid in terms of mass percentage.

[0050] The iPS cell liquid preparation steps are as follows:

[0051] That is, peripheral blood mononuclear cells (PBMC) are collected from human blood samples, infected with CytoTune-iPS2.0LG (cell reprogramming kit manufacturer: Japan ID Pharma Co.Ltd), and cultured under conditions of no supply and no serum After 21 days, human iPS cell colonies were formed, and then human iPS cell lines were established based on the human iPS cell colonies.

[0052] The specific preparation process is as follows: the peripheral blood sample is diluted with 1.5 times of PBS buffer (phosphate buffered saline solution, manufacturer: Thermo Fisher Scientific) and placed in lymphocyte separation medium (brand: Lymphoprep, manufacturer: Alere Technologies AS); then , centrifuge at 400g at room temperature for 30 min...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a skin care product using iPS cell culture fluid as a main ingredient and a preparation method thereof, and belongs to the technical field of cosmetics. The skin care product is prepared from 2 to 4 percent of iPS cell fluid and 96 to 98 percent of base fluid in percentage by mass. The skin care product using the iPS cell culture fluid as the main ingredient provided by theinvention has the advantages that growth factor functional ingredients of the cell fluid can be effectively maintained; the safety is very high; the skin feeling is comfortable; the moisture preservation and moisture retention effects are good; skin elasticity can be effectively maintained; and the skin care product is combined with the latest cell biology technology, and has good market prospects.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a skin care product mainly composed of iPS cell culture fluid and a preparation method thereof. Background technique [0002] With the advancement of biotechnology and the improvement of living standards, people's demand for high-end new skin care products using biotechnology is also increasing. iPS stem cells (induced pluripotent stem cells) are pluripotent stem cells formed by introducing transcription factors into differentiated adult cells of animals through reverse transcription, called induced pluripotent stem cells. Studies have shown that the culture medium of these pluripotent stem cells contains a variety of biologically active cell growth factors, which can effectively activate the activity of human adult stem cells, promote the proliferation of adult stem cells in hair follicles or the basal layer of the epidermis, and promote skin The self-renewal, regeneration an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/9794A61K8/9789A61Q19/00A61Q19/08
CPCA61K8/99A61K8/9794A61K8/9789A61Q19/00A61Q19/08A61K2800/805
Inventor 曹国华黄川靖杰
Owner 盛世荣恩生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap