Berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel and preparation method and application thereof

A technology of carboxymethyl chitosan and berberine hydrochloride, which is applied in the field of prevention or treatment of dairy cow mastitis, can solve the problems of easy flow of medicinal liquid and difficulty in continuously exerting medicinal effect, and achieves the effect of good bacteriostatic performance.

Active Publication Date: 2020-02-07
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In veterinary clinics, the most common way to prevent and treat mastitis in cows is to directly infuse the drug into the udder, but the liquid is easy to flow and it is difficult to continue to exert its effect

Method used

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  • Berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel and preparation method and application thereof
  • Berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel and preparation method and application thereof
  • Berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Preparation of berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel

[0035] (1) Take 0.1g berberine hydrochloride and 0.8g matrine in 94ml pure water, heat in a water bath, add 5g carboxymethyl chitosan powder to it after fully dissolving, stir evenly with a magnetic stirrer, place overnight, The mixed solution 1 was obtained after fully absorbing and dissolving, and refrigerated for later use.

[0036] (2) Weigh 22g of poloxamer 407 and 6g of poloxamer 188 and dissolve them in 72ml of pre-cooled pure water, stir them with a magnetic stirrer to obtain a mixed solution 2, and refrigerate for later use.

[0037] (3) Mix the mixed solution 1 and the mixed solution 2 in a disposable plate according to the ratio of 2:1, mix well with a magnetic stirrer to prevent the generation of air bubbles, and let it stand on a constant temperature heating platform at 37°C for 10-15min. After solidification, it is sterilized by Co60 irradiation and refrig...

Embodiment 2

[0038] Example 2 Preparation of berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel

[0039] (1) Take 0.05g berberine hydrochloride and 0.5g matrine in 94ml pure water, heat in a water bath, add 6g carboxymethyl chitosan powder to it after fully dissolving, stir evenly with a magnetic stirrer, place overnight, The mixed solution 1 was obtained after fully absorbing and dissolving, and refrigerated for later use.

[0040] (2) Weigh 10 g of Poloxamer 407 and 2 g of Poloxamer 407 and dissolve them in 88 ml of pre-cooled pure water, stir with a magnetic stirrer to obtain a mixed solution 2, and refrigerate for later use.

[0041] (3) Mix the mixed solution 1 and the mixed solution 2 in a disposable plate according to the ratio of 3:1, mix well with a magnetic stirrer to prevent the generation of air bubbles, and let it stand on a constant temperature heating platform at 37°C for 10-15min. After solidification, it is sterilized by Co60 irradiation and refrige...

Embodiment 3

[0042] Example 3 Preparation of berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel

[0043] (1) Weigh 0.5g berberine hydrochloride and 3g matrine in 94ml pure water, heat in a water bath, add 2g carboxymethyl chitosan powder to it after fully dissolving, stir evenly with a magnetic stirrer, place overnight, fully After absorbing and dissolving, the mixed solution 1 was obtained and refrigerated for later use.

[0044] (2) Weigh 30 g of Poloxamer 407 and 10 g of Poloxamer 407 and dissolve in 70 ml of pre-cooled pure water, stir with a magnetic stirrer to obtain a mixed solution 2, and refrigerate for later use.

[0045] (3) Mix the mixed solution 1 and the mixed solution 2 in a disposable plate according to the ratio of 5:3, and mix it with a magnetic stirrer to prevent the generation of air bubbles. Let it stand on a constant temperature heating platform at 37°C for 10-15min, and wait for it After solidification, it is sterilized by Co60 irradiation and...

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Abstract

The invention discloses berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel and a preparation method and application thereof. According to the berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel disclosed by the invention, berberine hydrochloride and matrine are used as model medicines, carboxymethyl chitosan and poloxamer are used as substrate materials, and temperature sensitive type composite hydrogel having a slow release function and a phase change characteristic is prepared. According to the in vitro bacteriostasis effect research result, the inventor finds that the berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel provided by the invention has favorable bacterium restraining properties on staphylococcus aureus, andhas potential to become a medicine for preventing or treating staphylococcus aureus infection, and a new prevention and treatment method is provided for mastitis of cows.

Description

technical field [0001] The invention relates to a composite hydrogel for preventing or treating cow mastitis, and the invention further relates to a berberine hydrochloride-matrine-carboxymethyl chitosan composite hydrogel for treating cow mastitis caused by Staphylococcus aureus The glue and a preparation method thereof belong to the field of prevention or treatment of cow mastitis. Background technique [0002] Dairy cow mastitis is one of the common diseases in large-scale dairy farming. The disease will interfere with the production activities of dairy cows, reduce milk production and quality of dairy products, and cause great harm to the dairy farming industry. There are many causes of mastitis in dairy cows, the main cause is that pathogenic bacteria cause ascending infection through mammary ducts, among which Staphylococcus aureus is one of the most important pathogenic bacteria. [0003] Staphylococcus aureus (usually referred to as Staphylococcus aureus) is one of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/36A61K47/10A61K31/4375A61P15/14A61P31/04
CPCA61K9/06A61K31/4375A61K47/10A61K47/36A61P15/14A61P31/04A61K2300/00
Inventor 张华童津津蔡丽蓉沈静张肇南张永红蒋林树
Owner BEIJING UNIV OF AGRI
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