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A kind of chitosan antibacterial microsphere capable of slow-release growth factor and its preparation method and application

A growth factor, chitosan technology, applied in antibacterial drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. Slow release effect, promotion of proliferation, migration and differentiation, effect of promotion of regeneration

Active Publication Date: 2021-09-03
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its direct application in the body, there are problems such as immune response and easy degradation, which limit its application in biomedicine.

Method used

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  • A kind of chitosan antibacterial microsphere capable of slow-release growth factor and its preparation method and application
  • A kind of chitosan antibacterial microsphere capable of slow-release growth factor and its preparation method and application
  • A kind of chitosan antibacterial microsphere capable of slow-release growth factor and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Preparation of Silica Template Microspheres

[0040]1) Preparation of silica template microspheres by a simple double-emulsion microfluidic device. In the double emulsion, the inner phase is a solution of silica particles with a concentration of 20% mass fraction, the particle size of the silica particles is 300nm-500nm, and the flow rate is 10ml / h; the outer phase is 350CS silicone oil, and the flow rate is 0.5ml / h. Based on the principle of compatibility, the external oil phase has a shear force on the silica particles, thereby forming microspheres in the microfluidic pipeline, and then collecting them in a container containing 500CS silicone oil.

[0041] 2) Put the container containing 500CS silicone oil in the previous step into a 75°C incubator overnight, and then pour out the silicone oil as much as possible. Absorb n-hexane (molecular weight 86.18g·mol) with a straw -1 ) pure solution to wash out the microspheres at the bottom of the container into a 5ml we...

Embodiment 2

[0051] Embodiment 2 antibacterial effect

[0052] (1) Preparation before the experiment

[0053] 1. Preparation of medium: Prepare 40-50ml of liquid agar medium (25g / L), pour it into a 50ml centrifuge tube and sterilize it in an autoclave for 4-6 hours.

[0054] 2. Preparation of bacteria liquid: Escherichia coli and Staphylococcus aureus obtained after clinical isolation and identification were inoculated into the culture medium, cultured with shaking (200rpm) at 36-37°C for 24 hours, and then eluted with sterile saline into a suspension (the bacterial concentration is 1:1000).

[0055] 3. material preparation: the chitosan porous gel microsphere that the step 2 preparation of embodiment 1 prepares

[0056] (2) Antibacterial experiment

[0057] 1. Inoculate the prepared chitosan gel microspheres and bacterial solution in a sterile 24-well plate, and place 1mg of different concentrations of chitosan gel microspheres and 200ul bacterial suspension in each well plate, each sh...

Embodiment 3

[0059] Embodiment 3 sustained-release medicine:

[0060] The chitosan antibacterial microspheres capable of slow-release growth factors prepared in Example 1 were placed in a small dish containing PBS, and each microsphere was set with 3 groups of parallel controls, a total of 15 groups. The fluorescence intensity of the microspheres was observed by a fluorescence microscope every 12 hours, and finally the data was analyzed by ImageJ software to make a curve. The results are shown in Figure 4 . Depend on Figure 4 It can be seen that the optional concentration of sodium alginate solution is 0.4% to 0.5%, and the optimal concentration is 0.5%.

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Abstract

The invention discloses a chitosan antibacterial microsphere capable of slow-releasing growth factors and a preparation method and application thereof. The chitosan antibacterial microspheres are composed of chitosan gel microspheres and calcium alginate gel loaded with basic fibroblast growth factor poured into the porous support of the chitosan gel microspheres. The chitosan antibacterial microspheres have good antibacterial, sustained release and wound repair functions, and can be used in the preparation of reagents or dressings for promoting wound healing.

Description

technical field [0001] The invention belongs to the field of new medical materials, and relates to a chitosan antibacterial microsphere capable of slow-release growth factors, a preparation method and application thereof. Background technique [0002] With the development of society, the prevalence of various acute traumas and various chronic diseases has gradually increased, leading to a gradual increase in patients with related wound injuries, and wound repair has always been an important and difficult point in clinical work. Clinically available treatments mainly include three categories: physical therapy, chemical and biological therapy, and active wound dressings. In terms of physical therapy, take typical negative pressure wound therapy as an example. Although this therapy can increase the oxygen tension of the wound, accelerate the formation of granulation tissue, reduce microcirculation disturbance, and improve blood supply, there are still complications after use. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K9/50A61K47/36A61K38/18A61P31/04A61P17/02A61L15/28A61L15/44
CPCA61K9/5036A61K38/1825A61L15/28A61L15/44A61L2300/252A61L2300/414A61L2300/622A61P17/02A61P31/04C08L5/08C08L5/04
Inventor 任建安陈灿文吴秀文王革非顾国胜
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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