Method for detecting fertilization ability of bovine frozen semen and its application

Abstract

The application discloses a method for detecting fertilization ability of bovine frozen semen and its application. The method for detecting the fertilization ability of bovine frozen semen comprises the following steps of performing indicator detection of the fertilization ability of bovine frozen semen on the sperms in the semen, and determining the fertilization ability of bovine frozen semen according to the fertilization ability indicators of bovine frozen semen, wherein the fertilization ability indicators of bovine frozen semen comprise sperm mobility, sperm motility, mitochondrial membrane channel pore activity, and sperm DNA damage. The method for detecting the fertilization ability of bovine frozen semen is to detect sperms by using sperm mobility, sperm motility, mitochondrial membrane channel pore activity and sperm DNA damage as indicators, and can quickly, accurately and effectively determine the fertilization ability of bovine frozen semen through the detection of the indicators, which provides a new objective and effective analysis method for the fertilization ability detection of bovine frozen semen, and is of great significance to the rapid customs inspection and biological breeding.

Description

technical field [0001] The application relates to the field of bovine semen activity detection, in particular to a method for detecting the fertilization ability of bovine frozen semen and its application. Background technique [0002] China is a large animal husbandry country. Importing high-quality livestock semen, especially frozen semen of cattle, is used for cross-breeding. The efficiency of rapid reproduction. [0003] The traditional methods of judging the fertilization ability of sperm mainly rely on the naked eye to observe the vitality and linear motion of the sperm under a common microscope, or to detect the viability and acrosome integrity rate of the sperm through staining. Whether it is microscope observation or staining detection, there is a problem of strong subjectivity, and the judgment of the test results is greatly affected by human factors; at the same time, the test results are also affected by the ambient temperature and sperm incubation time. [000...

AI Technical Summary

Problems solved by technology

Whether it is microscope observation or dyeing detection, there is a problem of strong subjectivity, and the judgment of the detection results is greatly affected by human factors; at the same time, the detection results are also affected by the ambient temperature, sperm incubation time, etc.

[0004] In recent years, bovine semen activity detection has been paid more and more attention. With the development of molecular biotechnology, various bovine semen activity detection methods emerge in endlessly, such as weak sperm-related gene expression detection methods, trying to judge bovine semen activity through genetic testing, and for example through sperm Linear motion speed or mitochondrial activity can be used to judge the activity of bovine semen; however, bovine semen activity parameters obtained by different detection methods have different evaluation results for the fertilization ability of bovine frozen semen, which is very important for the detection of bovine frozen semen fertilization ability, especially The rapid customs inspection of the fertilization ability of imported bovine frozen semen is extremely unfavorable

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