Il-8 inhibitors for use in treatment and/or prevention of bacterial secondary infections
A bacterial infection, 1. IL-8 technology, applied in the direction of antibacterial drugs, medical preparations containing active ingredients, anti-animal/human immunoglobulin, etc., can solve the problem of increased lethality
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Embodiment 1
[0129] Materials and methods
[0130] mouse
[0131] Male C57BL / 6J mice (8-12 weeks old) were obtained from the Central Animal Facility of the Federal University of Minas Gerais (CEBIO UFMG / Brazil) and maintained with free access to commercial food and water. All procedures described have been previously approved by the local animal ethics committee (CETEA / UFMG 13 / 2010 and 381 / 2015).
[0132] Bacteria and Virus Strains
[0133] Streptococcus pneumoniae (ATCC 6303 serotype 3) at 37°C and 5% CO 2 Grow on blood agar plates for 12 hours and prepare infection stocks as described (Tavares, L.P., et al., Inhibition of PDE4 During Pneumococcal Pneumonia Reduces Inflammation and Lung Injury in Mice. Am J Respir Cell Mol Biol, 2015). Inoculum is usually confirmed by plating a bacterial suspension.
[0134] Mouse-adapted influenza virus A / WSN / 33H1N1 (referred to herein as IAV) was grown in MDCK (Madin-Darby Canine Kidney) culture cells as described in (Garcia, C.C. et al., Platelet...
Embodiment 2
[0166] Role of the CXCR1 / 2 pathway in a mouse model of exacerbated pneumonia in combination virus (influenza A) infection and cigarette smoke exposure
[0167] Chronic obstructive pulmonary disease (COPD) is a health problem of global importance with increasing prevalence, accounting for approximately 5% of all deaths worldwide. The disease is characterized by persistent airflow limitation, which is usually progressive and is associated with an enhanced chronic inflammatory response of the lung airways to noxious particles or gases (Sethi, S. et al. Am.J.Med. 125, 1162 –1170; 2012). Smoking is a major risk factor for which there is no adequate treatment.
[0168] Increased neutrophils in lung tissue are a hallmark of COPD patients, usually accompanied by overproduction of inflammatory cytokines (such as TNF-α, IL-6 and IL-8, etc.). Although neutrophils are undoubtedly the main contributors to acute inflammation, several lines of evidence suggest that they may also contribute...
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