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Magnetic resonance imaging nanoscale contrast agent and preparation method and application thereof

A technology for magnetic resonance imaging and nano-contrast agent, which is applied in the field of contrast agent and can solve the problems of easy aggregation, high potential, and difficulty in expelling from the body.

Active Publication Date: 2020-02-14
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

but T 2 Contrast agent is a negative signal, presents a dark image, susceptible to interference from some blood pooling, calcification, and metal deposits
Therefore, everyone will focus on the small size Fe-type T 1 On the contrast agent, however, the small size makes it have a high potential and is easy to aggregate. If the method of loading is used to prevent aggregation, it is also easy to cause difficulty in excretion

Method used

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  • Magnetic resonance imaging nanoscale contrast agent and preparation method and application thereof
  • Magnetic resonance imaging nanoscale contrast agent and preparation method and application thereof
  • Magnetic resonance imaging nanoscale contrast agent and preparation method and application thereof

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preparation example Construction

[0030] The invention provides a method for preparing a magnetic resonance imaging nano-contrast agent, the preparation method comprising:

[0031] 1) Mix the iron source, citrate, and water and adjust the pH to acidity, then add urea and heat to reflux to obtain Fe 2 o 3 super nanoparticle Fe 2 o 3 SPs;

[0032] 2) Fe 2 o 3 SPs and bovine serum albumin (BSA) were contacted in solution to obtain magnetic resonance imaging nano-contrast agent.

[0033] In step 1) of the above preparation method, the amount of each material can be selected in a wide range, but in order to further improve the imaging effect of the prepared contrast agent, preferably, in step 1), iron source, citrate , water, urea dosage ratio is 1.6×10 -4 mol: 3.0×10 -4 -4.0×10 -4 mol: 80-150mL: 3×10 -4 mol-5×10 -4 mol.

[0034] In step 1) of the above-mentioned preparation method, the conditions of thermal reflux treatment can be selected in a wide range, but in order to further improve Fe 2 o 3 ...

Embodiment 1

[0045] 1) Fe 2 o 3 Preparation and purification of super nanoparticles (SPs):

[0046] Under the condition of constant stirring, add 3.4×10 -4 mol trisodium citrate, 1.6×10 -4 mol ferric chloride hexahydrate, use 0.1mol L -1 sodium hydroxide to adjust the pH to 6, fully shake, mix well and add 4×10 -4 mol urea, boiled and refluxed at 120°C for 24h. After the reaction was completed, the solution was cooled to 25° C., and samples were collected. Take 1 mL of the Fe prepared above 2 o 3 Add 3 mL of isopropanol to the SPs stock solution, mix well, centrifuge at 10,000 rpm for 10 min, discard the supernatant, and redisperse the obtained precipitate into water or freeze-dry it for later use.

[0047] 2) BSA-modified Fe 2 o 3 Preparation and purification of SPs:

[0048] to the purified Fe 2 o 3 BSA solution was added to the SPs precipitation (the mass ratio of Fe to BSA was 1:1, the solvent in the BSA solution was water, Fe 2 o 3 The ratio of SPs precipitation to ...

Embodiment 2

[0050] Under the condition of constant stirring, add 3.0×10 -4 mol trisodium citrate, 1.6×10 -4 mol ferric chloride hexahydrate, use 0.1mol L -1 sodium hydroxide to adjust the pH to 5.5, fully shake, mix well and add 3×10 -4 mol urea, boiled and refluxed at 110°C for 30h. After the reaction was completed, the solution was cooled to 25° C., and samples were collected. Take 1 mL of the Fe prepared above 2 o 3 Add 3 mL of isopropanol to the SPs stock solution, mix well, centrifuge at 10,000 rpm for 10 min, discard the supernatant, and redisperse the obtained precipitate into water or freeze-dry it for later use.

[0051] 2) BSA-modified Fe 2 o 3 Preparation and purification of SPs:

[0052] to the purified Fe 2 o 3 BSA solution was added to the SPs precipitation (the mass ratio of Fe to BSA was 1:0.8, the solvent in the BSA solution was water, Fe 2 o 3 The ratio of SPs precipitation to BSA solution is 1mg:1mL), re-disperse; after mixing evenly, put it on a shaker ...

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Abstract

The invention discloses a magnetic resonance imaging nanoscale contrast agent and a preparation method and application thereof. The preparation method includes the steps: (1) an iron source, citratesand water are mixed, and pH is adjusted to acidity, then, urea is added, and heating reflux is conducted to obtain Fe2O3 super nanoparticles (Fe2O3 SPs); and (2) the Fe2O3 SPs and bovine serum albumin(BSA) are subjected to a contact reaction during dissolving, so that the magnetic resonance imaging nanoscale contrast agent is obtained. The magnetic resonance imaging nanoscale contrast agent belongs to T1 contrast agents, has the advantages of being excellent in imaging effect and biosafety and easy to metabolize, and accordingly can be applied in magnetic resonance imaging; and meanwhile, thepreparation method of the magnetic resonance imaging nanoscale contrast agent has the advantages that raw materials are easy to obtain, operation is convenient, and conditions are mild.

Description

technical field [0001] The present invention relates to a contrast agent, in particular to a magnetic resonance imaging nano contrast agent and its preparation method and application. Background technique [0002] Magnetic resonance imaging technology has become a powerful tool for current medical diagnosis due to its non-invasiveness and radiation-free. In order to achieve better imaging effects, currently about 40-50% contrast agents are used for MRI detection. MRI contrast agents are usually classified into longitudinal (T 1 ) contrast agent and lateral (T 2 ) There are two types of contrast agents. [0003] Commonly used T 1 Contrast agent is Gd-, Mn-type contrast agent, it is a kind of positive signal, presents a bright image, and the imaging effect is good, but it is very dangerous. Once dissociated and accumulated in the body, it will cause serious diseases and even death, such as Gd contrast agent The use of drugs can cause nephrogenic systemic fibrosis (NSF) in ...

Claims

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Application Information

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IPC IPC(8): A61K49/14
CPCA61K49/143
Inventor 夏云生马明柔罗荣韦妹妹张冰洁朱慧凌云云汪宜王标李瑞瑞王鹤孙金霞凌静郑云云王越唐冬山
Owner ANHUI NORMAL UNIV
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